Effect of paricalcitol and enalapril on renal inflammation/oxidative stress in atherosclerosis

Husain, Kazim; Suarez, Edu; Isidro, Angel A.; Hernandez, Wilfredo; Ferder, León

doi:10.4331/wjbc.v6.i3.240

Cited by 14 publications

(8 citation statements)

References 65 publications

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“…For example, a phenotypic screen of 37 strains of mice22 using the VPR tail‐cuff system (same as in this study) reported a 100±2 to 133±3 mm Hg range of SBPs for those animals. Other laboratories had comparable results using the same39 or different tail‐cuff systems 40, 41, 42. On the other hand, studies that have not concentrated on the tail‐cuff technique show elevated central blood pressure as measured by telemetry during the restraint in mice24, 43 and rats 36, 37, 38.…”

Section: Discussionmentioning

confidence: 92%

“…Other laboratories had comparable results using the same 39 or different tail-cuff systems. [40][41][42] On the other hand, studies that have not concentrated on the tail-cuff technique show elevated central blood pressure as measured by telemetry during the restraint in mice 24,43 and rats. [36][37][38] Ours is the first study to show that there is a highly significant difference between simultaneous tail-cuff and central blood pressure telemetry readings.…”

Section: Discussionmentioning

confidence: 99%

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Tail‐Cuff Technique and Its Influence on Central Blood Pressure in the Mouse

Wilde

Aubdool

Thakore

et al. 2017

JAHA

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BackgroundReliable measurement of blood pressure in conscious mice is essential in cardiovascular research. Telemetry, the “gold‐standard” technique, is invasive and expensive and therefore tail‐cuff, a noninvasive alternative, is widely used. However, tail‐cuff requires handling and restraint during measurement, which may cause stress affecting blood pressure and undermining reliability of the results.Methods and ResultsC57Bl/6J mice were implanted with radio‐telemetry probes to investigate the effects of the steps of the tail‐cuff technique on central blood pressure, heart rate, and temperature. This included comparison of handling techniques, operator's sex, habituation, and influence of hypertension induced by angiotensin II. Direct comparison of measurements obtained by telemetry and tail‐cuff were made in the same mouse. The results revealed significant increases in central blood pressure, heart rate, and core body temperature from baseline following handling interventions without significant difference among the different handling technique, habituation, or sex of the investigator. Restraint induced the largest and sustained increase in cardiovascular parameters and temperature. The tail‐cuff readings significantly underestimated those from simultaneous telemetry recordings; however, “nonsimultaneous” telemetry, obtained in undisturbed mice, were similar to tail‐cuff readings obtained in undisturbed mice on the same day.ConclusionsThis study reveals that the tail‐cuff technique underestimates the core blood pressure changes that occur simultaneously during the restraint and measurement phases. However, the measurements between the 2 techniques are similar when tail‐cuff readings are compared with telemetry readings in the nondisturbed mice. The differences between the simultaneous recordings by the 2 techniques should be recognized by researchers.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Tail‐Cuff Technique and Its Influence on Central Blood Pressure in the Mouse

Wilde

Aubdool

Thakore

et al. 2017

JAHA

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“…Moreover, combining lisinopril and L-arginine was reported be effective in reversing proteinuria and protecting against renal function impairment in passive Heymann nephritis rats (Zoja et al, 2003). Likewise, in a study by Husain et al (2015), combined treatment of ApoE-knockout mice with paricalcitol and enalapril significantly ameliorated the renal inflammation and oxidative stress.…”

Section: Ace Inhibitors In the Treatment Of Nephritismentioning

confidence: 95%

The potential therapeutic use of renin–angiotensin system inhibitors in the treatment of inflammatory diseases

Ranjbar

Shafiee

Hesari

et al. 2018

Journal Cellular Physiology

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Inflammation is a normal part of the immune response to injury or infection but its dysregulation promotes the development of inflammatory diseases, which cause considerable human suffering. Nonsteroidal anti-inflammatory agents are the most commonly prescribed agents for the treatment of inflammatory diseases, but they are accompanied by a broad range of side effects, including gastrointestinal and cardiovascular events. The renin-angiotensin system (RAS) is traditionally known for its role in blood pressure regulation. However, there is increasing evidence that RAS signaling is also involved in the inflammatory response associated with several disease states. Angiotensin II increases blood pressure by binding to angiotensin type 1 (AT ) receptor, and direct renin inhibitors, angiotensin-converting enzyme (ACE) inhibitors and AT receptor blockers (ARBs) are clinically used as antihypertensive agents. Recent data suggest that these drugs also have anti-inflammatory effects. Therefore, this review summarizes these recent findings for the efficacy of two of the most widely used antihypertensive drug classes, ACE inhibitors and ARBs, to reduce or treat inflammatory diseases such as atherosclerosis, arthritis, steatohepatitis, colitis, pancreatitis, and nephritis.

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“…In addition, Husain et al . [21] reported that paricalcitol and enalapril alone or in combination ameliorated the blood pressure and renal lipid peroxidation (LPO), antioxidant defense system and inflammatory proteins in ApoE-deficient atherosclerotic mice; the effect paricalcitol and enalapril in combination was the most potent on renal oxidative stress and antioxidant defense system. Sagar et al .…”

Section: Introductionmentioning

confidence: 99%

Effects of enalapril and paricalcitol treatment on diabetic nephropathy and renal expressions of TNF-α, p53, caspase-3 and Bcl-2 in STZ-induced diabetic rats

et al. 2019

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This study aimed to assess the renopreventive effect of enalapril and/or paricalcitol on streptozotocin (STZ) diabetes-induced nephropathy and to elucidate their mechanisms of action through investigation of the effects on renal oxidative stress, antioxidant defense system and expressions of TNF-α, p53, caspase-3, and Bcl-2. Diabetes mellitus was induced in fasting male Wistar rats by single intraperitoneal injection of STZ (45 mg /kg b.w.) dissolved in citrate buffer (pH 4.5). Ten days after STZ injection, the diabetic rats were treated with enalapril (25 mg/l of drinking water) and/or paricalcitol (8 μg/kg b.w. per os) dissolved in 5% DMSO daily for 4 weeks. The obtained data revealed that the treatment of diabetic Wistar rats with enalapril and/or paricalcitol led to significant decreases in the elevated serum urea, uric acid, creatinine, sodium and potassium levels; thereby reflecting the improvement of the impaired kidney function. The deteriorated kidney lipid peroxidation, GSH content and GST and catalase activities in diabetic rats were significantly ameliorated as a result of treatment with enalapril and/or paricalcitol. The elevated fasting and post-prandial serum glucose levels and the lowered serum insulin and C-peptide levels were also improved. The treatment with enalapril and paricalcitol in combination was the most potent in decreasing the elevated serum glucose levels. Moreover, the treatment of diabetic rats successfully prevented the diabetes-induced histopathological deleterious changes of kidney and islets of Langerhans of pancreas. In association, the immunohistochemically detected pro-inflammatory cytokine, TNF-α, and apoptotic mediators, p53 and caspase-3, were remarkably decreased in kidney of diabetic rats as a result of treatment while the expression of anti-apoptotic protein Bcl-2 was increased. Based on these findings, it can be concluded that enalapril and paricalcitol alone or in combination can prevent STZ diabetes-induced nephropathy through amelioration of the glycemic state and antioxidant defense system together with the suppression of oxidative stress, inflammation and apoptosis. However, the treatment of diabetic rats with enalapril and paricalcitol in combination has no further significant improvement effects on renal function and damage when compared with enalapril or paclitaxel treated diabetic groups.

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Effect of paricalcitol and enalapril on renal inflammation/oxidative stress in atherosclerosis

Abstract: Paricalcitol and enalapril combo treatment ameliorates renal inflammation as well as oxidative stress in atherosclerotic animals.

Cited by 14 publications

References 65 publications

Tail‐Cuff Technique and Its Influence on Central Blood Pressure in the Mouse

Tail‐Cuff Technique and Its Influence on Central Blood Pressure in the Mouse

The potential therapeutic use of renin–angiotensin system inhibitors in the treatment of inflammatory diseases

Effects of enalapril and paricalcitol treatment on diabetic nephropathy and renal expressions of TNF-α, p53, caspase-3 and Bcl-2 in STZ-induced diabetic rats

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