Tetra-primer ARMS-PCR identified four pivotal genetic variations in bovine PNPLA3 gene and its expression patterns

Wang, Zi-nian; Cai, Hanfang; Li, Mingxun; Cao, Xiukai; Lan, Xianyong; Lei, Chuzhao; Chen, Hong

doi:10.1016/j.gene.2015.08.047

Cited by 2 publications

(2 citation statements)

References 39 publications

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“…[29][30][31] The small-molecules interior of the PA membrane can cause the increase of the polymer chains and subsequently the change of the system's volume. 32 Furthermore, a large amount of smallmolecules can result in the deterioration of the material's stability and subsequently caused swelling of the material when the culture period was long, and even destroy the original membrane structure. 32 Due to the presence of some noncrystalline polyamide polar genes in the nylon materials, there were large molecular weight amide-containing compounds in this part of the amorphous polyamide, which could be correlated with the presence of highly polar water molecules.…”

Section: Biomass Accumulation On Different Membrane Surfacesmentioning

confidence: 99%

“…32 Furthermore, a large amount of smallmolecules can result in the deterioration of the material's stability and subsequently caused swelling of the material when the culture period was long, and even destroy the original membrane structure. 32 Due to the presence of some noncrystalline polyamide polar genes in the nylon materials, there were large molecular weight amide-containing compounds in this part of the amorphous polyamide, which could be correlated with the presence of highly polar water molecules. This may have caused the deterioration of the material's stability and subsequently caused swelling of the material when the culture period was long.…”

Section: Biomass Accumulation On Different Membrane Surfacesmentioning

confidence: 99%

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Section: Biomass Accumulation On Different Membrane Surfacesmentioning

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confidence: 99%

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Development of a tetra-primer ARMS–PCR for identification of sika and red deer and their hybrids

Ke-xin,

Xiang,

Qing-qing

et al. 2023

ANAL. SCI.

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Accurate identification of deer-derived components is significant in food and drug authenticity. Over the years, several methods have been developed to authenticate these products; however, identifying whether female deer products are hybrids is challenging. In this study, the zinc finger protein X-linked (ZFX) gene sequences of sika deer (Cervus nippon), red deer (Cervus elaphus) and their hybrid offspring were amplified and sequenced, the X221 and X428 species-specific single nucleotide polymorphisms (SNP) loci were verified, and a tetra-primer amplification refractory mutation system (T-ARMS–PCR) assay was developed to identify the parent-of-origin of female sika deer, red deer, and their hybrid deer. The T-ARMS–PCR developed based on the X221 locus could identify sika deer, red deer, and their hybrid offspring according to the presence or absence of PCR product sizes of 486 bp, 352 bp, and 179 bp, respectively, just as X428 locus could identify sika deer, red deer, and their hybrid offspring according to the presence or absence of PCR product sizes of 549 bp, 213 bp, and 383 bp, respectively. Forty products labeled deer-derived ingredients randomly purchased were tested using this assay, and the results showed that the identification results based on the two SNP loci were utterly consistent with the actual sources. In addition, this method was found to be accurate, simple, convenient, and with high specificity, thus providing an essential technical reference for deer product species identification. It is also an important supplement to the identification methods of the original ingredients of existing deer products. Graphical abstract

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Tetra-primer ARMS-PCR identified four pivotal genetic variations in bovine PNPLA3 gene and its expression patterns

Cited by 2 publications

References 39 publications

Evaluation of the performance of different membrane materials for microalgae cultivation on attached biofilm reactors

Evaluation of the performance of different membrane materials for microalgae cultivation on attached biofilm reactors

Development of a tetra-primer ARMS–PCR for identification of sika and red deer and their hybrids

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