2,3-Dimethoxy-2′-hydroxychalcone ameliorates TNF-α-induced ICAM-1 expression and subsequent monocyte adhesiveness via NF-kappaB inhibition and HO-1 induction in HaCaT cells

Kim, Hye-Jin; Youn, Gi Soo; An, Soo Yeon; Kwon, Hyeok Yil; Choi, Soo Young; Park, Jinseu

doi:10.5483/bmbrep.2016.49.1.141

Cited by 16 publications

(17 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Against the inflammatory cytokines (i.e., tumor necrosis factor‐alpha [TNF‐α] and interferon‐gamma), ICAM‐1 expression levels can be increased in the keratinocytes of psoriatic lesions (Kim et al, ). The upregulation of ICAM‐1 expression implies the progress of inflammatory disorders, and ICAM‐1 may act as a critical role in the interactions between keratinocytes and leukocytes (Kim et al, ; Matsunaga, Katayama, Yokozeki, & Nishioka, ). As shown in Figure , ICAM‐1 expression level in IMQ group was significantly higher than that of control group.…”

Section: Resultsmentioning

confidence: 99%

Antiproliferation of keratinocytes and alleviation of psoriasis by the ethanol extract of Artemisia capillaris

Lee

Nam

Hong³

et al. 2018

Phytotherapy Research

View full text Add to dashboard Cite

The therapeutic potentials of the ethanol extract of Artemisia capillaris (ACE) for psoriasis were verified in HaCaT cells (as an immortalized human keratinocyte cell line) and imiquimod (IMQ)-induced psoriasis-like mouse models. In HaCaT cells, IC value of ACE was 37.5 μg/ml after incubating for 72 hr. The antiproliferation activity of ACE in HaCaT cells was further verified by apoptosis assays. The percentage of apoptotic population in ACE-treated group was significantly higher than that of control group (p < .05). The result of cell cycle arrest assay also supported the observed antiproliferation efficacy of ACE in HaCaT cells. In IMQ-induced psoriasis-like mouse models, the Psoriasis Area and Severity Index score of ACE (50 mg/ml; ACE50)-treated group was significantly lower than that of IMQ group on Day 4 (p < .05). After topical application of ACE on psoriasis-like lesion for 4 days, the epidermal thickness of (IMQ + ACE50) group was significantly lower than that of IMQ group (p < .05). The expression levels of Ki-67 and intracellular adhesion molecule-1 in excised skin tissues of (IMQ + ACE50) group were also lower than those of IMQ group. All these findings suggest that ACE can be used as a promising antipsoriatic agent.

show abstract

Section: Resultsmentioning

confidence: 99%

Antiproliferation of keratinocytes and alleviation of psoriasis by the ethanol extract of Artemisia capillaris

Lee

Nam

Hong³

et al. 2018

Phytotherapy Research

View full text Add to dashboard Cite

show abstract

“…Of note, HO-1 expression has been reported to exert an anti-inflammatory response in keratinocytes, and attenuates AD-like lesions via its protective effects against inflammatory skin diseases (7,9,15). HO-1 expression is regulated by inflammation-associated cytokines and chemokines associated with the development of AD (15).…”

Section: Discussionmentioning

confidence: 99%

“…ICAM-1 (7)(8)(9). Additionally, TNF-α-induced expression of inflammatory mediators within keratinocytes simultaneously activates inflammatory signaling pathways, nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs), resulting in aggravated inflammation (10).…”

Section: Spilanthol Inhibits Tnf-α-induced Icam-1 Expression and Pro-mentioning

confidence: 99%

Spilanthol inhibits TNF‑α‑induced ICAM‑1 expression and pro‑inflammatory responses by inducing heme oxygenase‑1 expression and suppressing pJNK in HaCaT keratinocytes

Huang

Chang

et al. 2018

Mol Med Report

View full text Add to dashboard Cite

Spilanthol has been reported to possess antioxidant, anti‑inflammatory, antimicrobial and antinociceptive properties. At present, the literature has reported the beneficial role of spilanthol on tumor necrosis factor‑α (TNF‑α)‑stimulated HaCaT cells. The present study investigated the effects of spilanthol on the expression of TNF‑α‑induced intercellular adhesion molecule 1 (ICAM‑1) and cyclooxygenase (COX)‑2 in the human keratinocyte cell line HaCaT. Cells were pretreated with various concentrations of spilanthol (10‑150 µM) followed by TNF‑α to induce inflammation. Pretreatment with spilanthol decreased TNF‑α‑induced COX‑2 expression by western blotting and suppressed the expression of pro‑inflammatory mediators, including interleukin (IL)‑6, IL‑8 and monocyte chemotactic protein 1 using ELISA. Spilanthol also decreased the expression of TNF‑α‑induced ICAM‑1 protein and mRNA assay by western blotting and RT‑qPCR, respectively, in addition to the monocyte adhesiveness of HaCaT cells. Furthermore, spilanthol significantly suppressed the phosphorylation of c‑Jun N‑terminal kinase (JNK), while pretreatment with spilanthol enhanced heme oxygenase (HO)‑1 protein expression by western blotting. These results demonstrated that spilanthol may exert its anti‑inflammatory activity by suppressing the TNF‑α‑induced expression of ICAM‑1, COX‑2 and pro‑inflammatory mediators by enhancing that of HO‑1, and inhibiting the activation of the phosphorylated JNK signaling pathway. It is hypothesized that spilanthol may be a natural anti‑inflammatory drug to attenuate skin inflammatory disease.

show abstract

“…Samples were subjected to 10% SDS-polyacrylamide gel electrophoresis and subsequently transferred onto nitrocellulose membranes, detected by enhanced chemiluminescence according to the manufacturer's instructions (Amersham, Buckinghamshire, UK), and analyzed using a Molecular Imager ChemiDoc XRS System (Bio-Rad, Hercules, CA) as described elsewhere (Eom et al 2015;Kim et al 2016). The following antibodies were used for Western blotting: anti-caspase-3, anti-calpain, anti-Bax, anti-Bcl-2, anti-iNOS, antinNOS, anti-Nrf2, anti HO-1, and anti-GCLC.…”

Section: Western Blottingmentioning

confidence: 99%

“…All tests were done in accordance with the manufacturer's instructions and as described elsewhere (Ju et al 2015;Kim et al 2016).…”

Section: Measurement Of Cytokines and Nomentioning

confidence: 99%

N-Adamantyl-4-Methylthiazol-2-Amine Attenuates Glutamate-Induced Oxidative Stress and Inflammation in the Brain

et al. 2017

Self Cite

View full text Add to dashboard Cite

In this study, we explored the possible mechanisms underlying the neuroprotective and anti-oxidative effects of N-adamantyl-4-methylthiazol-2-amine (KHG26693) against in vivo glutamate-induced toxicity in the rat cerebral cortex. Our results showed that pretreatment with KHG26693 significantly attenuated glutamate-induced elevation of lipid peroxidation, tumor necrosis factor-α, interferon gamma, IFN-γ, interleukin-1β, nitric oxide, reactive oxygen species, NADPH oxidase, caspase-3, calpain activity, and Bax. Furthermore, KHG26693 pretreatment attenuated key antioxidant parameters such as levels of superoxide dismutase, catalase, glutathione, and glutathione reductase. KHG26693 also attenuated the protein levels of inducible nitric oxide synthase, neuronal nitric oxide synthase, nuclear factor erythroid 2-related factor 2, heme oxygenase-1, and glutamate cysteine ligase catalytic subunit caused by glutamate toxicity. Finally, KHG26693 mitigated glutamate-induced changes in mitochondrial ATP level and cytochrome oxidase c. Thus, KHG26693 functions as neuroprotective and anti-oxidative agent against glutamate-induced toxicity through its antioxidant and anti-inflammatory activities in rat brain at least in part.

show abstract

2,3-Dimethoxy-2′-hydroxychalcone ameliorates TNF-α-induced ICAM-1 expression and subsequent monocyte adhesiveness via NF-kappaB inhibition and HO-1 induction in HaCaT cells

Cited by 16 publications

References 25 publications

Antiproliferation of keratinocytes and alleviation of psoriasis by the ethanol extract of Artemisia capillaris

Antiproliferation of keratinocytes and alleviation of psoriasis by the ethanol extract of Artemisia capillaris

Spilanthol inhibits TNF‑α‑induced ICAM‑1 expression and pro‑inflammatory responses by inducing heme oxygenase‑1 expression and suppressing pJNK in HaCaT keratinocytes

N-Adamantyl-4-Methylthiazol-2-Amine Attenuates Glutamate-Induced Oxidative Stress and Inflammation in the Brain

Contact Info

Product

Resources

About