Genome sequence and comparative analysis of a putative entomopathogenic Serratia isolated from Caenorhabditis briggsae

Abebe-Akele, Feseha; Tisa, Louis S.; Cooper, Vaughn S.; Hatcher, Philip J.; Abebe, Eyualem; Thomas, W. Kelley

doi:10.1186/s12864-015-1697-8

Cited by 22 publications

(21 citation statements)

References 106 publications

(111 reference statements)

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“…marcescens (including the type strain), and two genomes of other Serratia species. Interestingly, the closest genome match (98.49% similarity, as shown by AAI identity scores listed in the S1 Dataset) of Ss1 is to Serratia sp SCBI [31,32], a pathogen of the lepidopteran Galleria mellonella (honey comb moth), which is a pest of honey bee colonies. The complete dendrogram that includes the phylogenetic relationships of all 42 Serratia genomes based on AAI analysis is also provided as an unrooted phylogenetic tree (S2 Fig), and their distance scale values are shown in S1 Dataset.…”

Section: Resultsmentioning

confidence: 99%

Sepsis and Hemocyte Loss in Honey Bees (Apis mellifera) Infected with Serratia marcescens Strain Sicaria

et al. 2016

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Global loss of honey bee colonies is threatening the human food supply. Diverse pathogens reduce honey bee hardiness needed to sustain colonies, especially in winter. We isolated a free-living Gram negative bacillus from hemolymph of worker honey bees (Apis mellifera) found separated from winter clusters. In some hives, greater than 90% of the dying bees detached from the winter cluster were found to contain this bacterium in their hemolymph. Throughout the year, the same organism was rarely found in bees engaged in normal hive activities, but was detected in about half of Varroa destructor mites obtained from colonies that housed the septic bees. Flow cytometry of hemolymph from septic bees showed a significant reduction of plasmatocytes and other types of hemocytes. Interpretation of the16S rRNA sequence of the bacterium indicated that it belongs to the Serratia genus of Gram-negative Gammaproteobacteria, which has not previously been implicated as a pathogen of adult honey bees. Complete genome sequence analysis of the bacterium supported its classification as a novel strain of Serratia marcescens, which was designated as S. marcescens strain sicaria (Ss1). When compared with other strains of S. marcescens, Ss1 demonstrated several phenotypic and genetic differences, including 65 genes not previously found in other Serratia genomes. Some of the unique genes we identified in Ss1 were related to those from bacterial insect pathogens and commensals. Recovery of this organism extends a complex pathosphere of agents which may contribute to failure of honey bee colonies.

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Section: Resultsmentioning

confidence: 99%

Sepsis and Hemocyte Loss in Honey Bees (Apis mellifera) Infected with Serratia marcescens Strain Sicaria

et al. 2016

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“…Bacterial strains under investigation in this study were derived from S. proteamaculans 568, P. putida GB-1 and P. putida B6–2 (Abebe-Akele et al, 2015; Tang et al, 2011; Wu et al, 2011). Cultivation of these organisms was performed using Luria-Bertani media (LB) at 30°C.…”

Section: Star Methodsmentioning

confidence: 99%

Bifunctional Immunity Proteins Protect Bacteria against FtsZ-Targeting ADP-Ribosylating Toxins

Ting

Bosch

Mangiameli

et al. 2018

Cell

111

110

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Summary ADP-ribosylation of proteins can profoundly impact their function and serves as an effective mechanism by which bacterial toxins impair eukaryotic cell processes. Here we report the discovery that bacteria also employ ADP-ribosylating toxins against each other during interspecies competition. We demonstrate that one such toxin from Serratia proteamaculans interrupts the division of competing cells by modifying the essential bacterial tubulin-like protein, FtsZ, adjacent to its protomer interface, blocking its capacity to polymerize. The structure of the toxin in complex with its immunity determinant revealed two distinct modes of inhibition: active site occlusion and enzymatic removal of ADP-ribose modifications. We show that each is sufficient to support toxin immunity; however, the latter additionally provides unprecedented broad protection against non-cognate ADP-ribosylating effectors. Our findings reveal how an interbacterial arms race has produced a unique solution for safeguarding the integrity of bacterial cell division machinery against inactivating post-translational modifications.

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“…In particular, the discovery of symbionts belonging to a unique genus that are present in contrastingly different stages of genome reduction is shedding light on genome reduction and its link to phenotypic traits. Such examples include Arsenophonus , Coxiella , Serratia , and Sodalis symbionts. Particularly, Coxiella and Serratia symbionts from ticks and aphids, respectively, offer the unique opportunity to dissect the genome‐reduction process within a single symbiotic lineage and closely related hosts (ticks or aphids).…”

Section: Perspectivesmentioning

confidence: 99%

“…” was changed to “[…] and Aphididae (aphids) ” was changed to “[…] proposed for Carsonella in psyllids”; on page 18, “[…] Serratia ,” was changed to “[…] Serratia ,”; and “ Sodalis ” was changed to “ Sodalis ”. ]…”

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confidence: 99%

Dissecting genome reduction and trait loss in insect endosymbionts

Latorre

Manzano-Marı́n

2016

Annals of the New York Academy of Sciences

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Symbiosis has played a major role in eukaryotic evolution beyond the origin of the eukaryotic cell. Thus, organisms across the tree of life are associated with diverse microbial partners, conferring to the host new adaptive traits that enable it to explore new niches. This is the case for insects thriving on unbalanced diets, which harbor mutualistic intracellular microorganisms, mostly bacteria that supply them with the required nutrients. As a consequence of the lifestyle change, from free-living to host-associated mutualist, a bacterium undergoes many structural and metabolic changes, of which genome shrinkage is the most dramatic. The trend toward genome size reduction in endosymbiotic bacteria is associated with large-scale gene loss, reflecting the lack of an effective selection mechanism to maintain genes that are rendered superfluous by the constant and rich environment provided by the host. This genome-reduction syndrome is so strong that it has generated the smallest bacterial genomes found to date, whose gene contents are so limited that their status as cellular entities is questionable. The recent availability of data on several endosymbiotic bacteria is enabling us to form a comprehensive picture of the genome-reduction process and the phenotypic consequences for the dwindling symbiont.

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Genome sequence and comparative analysis of a putative entomopathogenic Serratia isolated from Caenorhabditis briggsae

Cited by 22 publications

References 106 publications

Sepsis and Hemocyte Loss in Honey Bees (Apis mellifera) Infected with Serratia marcescens Strain Sicaria

Sepsis and Hemocyte Loss in Honey Bees (Apis mellifera) Infected with Serratia marcescens Strain Sicaria

Bifunctional Immunity Proteins Protect Bacteria against FtsZ-Targeting ADP-Ribosylating Toxins

Dissecting genome reduction and trait loss in insect endosymbionts

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