A pin-fasten grafting method provides a non-sterile and highly efficient method for grafting Arabidopsis at diverse developmental stages

Huang, Nien‐Chen; Yu, Tien‐Shin

doi:10.1186/s13007-015-0081-7

Cited by 20 publications

(13 citation statements)

References 31 publications

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“…In our experience, the conventional micrografting method requires much practice to achieve a high success rate. Several papers have reported modifications that increase the success rate of micrografting by modifying equipment and procedures (Notaguchi et al, 2009), using an agar-based support (Marsch-Mart ınez et al, 2013), and using a fine pin to fix plants in position (Huang and Yu, 2015). Nevertheless, micrografting is difficult, particularly for beginners, which hampers its adoption.…”

Section: Introductionmentioning

confidence: 99%

Micrografting device for testing systemic signaling in Arabidopsis

et al. 2020

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Summary Grafting techniques have been applied in studies of systemic, long‐distance signaling in several model plants. Seedling grafting in Arabidopsis, known as micrografting, enables investigation of the molecular mechanisms of systemic signaling between shoots and roots. However, conventional micrografting requires a high level of skill, limiting its use. Thus, an easier user‐friendly method is needed. Here, we developed a silicone microscaled device, the micrografting chip, to obviate the need for training and to generate less stressed and more uniformly grafted seedlings. The chip has tandemly arrayed units, each of which consists of a seed pocket for seed germination and a micro‐path with pairs of pillars for hypocotyl holding. Grafting, including seed germination, micrografting manipulation and establishment of tissue reunion, is performed on the chip. Using the micrografting chip, we evaluated the effect of temperature and the carbon source on grafting, and showed that a temperature of 27°C and a sucrose concentration of 0.5% were optimal. We also used the chip to investigate the mechanism of systemic signaling of iron status using a quadruple nicotianamine synthase (nas) mutant. The constitutive iron‐deficiency response in the nas mutant because of iron accumulation in shoots was significantly rescued by grafting of wild‐type shoots or roots, suggesting that shoot‐ and root‐ward translocation of nicotianamine–iron complexes and/or nicotianamine is essential for iron mobilization. Thus, our micrografting chip will promote studies of long‐distance signaling in plants.

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Section: Introductionmentioning

confidence: 99%

Micrografting device for testing systemic signaling in Arabidopsis

et al. 2020

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“…Much of this work was performed on non‐model species, limiting the mechanistic insight on graft formation. Recently, though, new techniques have allowed grafting of hypocotyls, meristems, cotyledons, and inflorescences of the model plant Arabidopsis thaliana (Rhee & Somerville, ; Turnbull, Booker, & Leyser, ; Chen, Komives, & Schroeder, ; Flaishman, Loginovsky, Golobowich, & Lev‐Yadun, ; Nisar, Verma, Pogson, & Cazzonelli, ; Yoo, Hong, Jung, & Ahn, ; Huang & Yu, ), which has provided important insights into our understanding of grafting and regeneration.…”

Section: Introductionmentioning

confidence: 99%

Plant grafting: insights into tissue regeneration

2016

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For millennia, people have cut and joined different plants together through a process known as grafting. The severed tissues adhere, the cells divide and the vasculature differentiates through a remarkable process of regeneration between two genetically distinct organisms as they become one. Grafting is becoming increasingly important in horticulture where it provides an efficient means for asexual propagation. Grafting also combines desirable roots and shoots to generate chimeras that are more vigorous, more pathogen resistant and more abiotic stress resistant. Thus, it presents an elegant and efficient way to improve plant productivity in vegetables and trees using traditional techniques. Despite this horticultural importance, we are only beginning to understand how plants regenerate tissues at the graft junction. By understanding grafting better, we can shed light on fundamental regeneration pathways and the basis for self/non‐self recognition. We can also better understand why many plants efficiently graft whereas others cannot, with the goal of improving grafting so as to broaden the range of grafted plants to create even more desirable chimeras. Here, I review the latest findings describing how plants graft and provide insight into future directions in this emerging field.

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“…Multiple types of grafting methods have been reported for A. thaliana [26, 27, 30, 31]. In the present study, the method of cut-in grafting was only used for A/E grafts and the method of hypocotyl–hypocotyl flat-surface cutting was used for A/A, E/E, and E/A (Fig.…”

Section: Introductionmentioning

confidence: 87%

Methods for grafting Arabidopsis thaliana and Eutrema salsugineum

Sun

FuLin

et al. 2019

Plant Methods

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Background Grafting, an ancient agronomic technique, is an artificial mode of asexual reproduction in plants. Recently, grafting research has gradually shifted from modifying agronomic traits to the study of molecular mechanism. Grafting is an excellent tool to study long-range signaling processes in plants. And the grafting between species will help elucidate the molecular mechanisms underlying contrasting differences between different species. Arabidopsis thaliana is a salt-sensitive model glycophyte and Eutrema salsugineum (previously Thellungiella salsuginea , salt cress) is a salt-tolerant model halophyte. Successful grafting of these two model plants will help further study the physiological and molecular mechanisms underlying salt tolerance in plants. The aim of this study was to demonstrate two sterile micro-grafting methods for Arabidopsis and salt cress. Results We developed the methods for sterile grafting between A. thaliana and E. salsugineum ; this is the first report on inter-generic grafting between Arabidopsis and Eutrema . The method involves cut-in grafting under sterile conditions. The grafted plant part was placed in half strength Murashige and Skoog medium with 1% agar and 1% sugar, and then cultured vertically with 22 °C/18 °C short-day/night cycles. The plants were then transferred to half strength Hoagland nutrient solution for hydroponics. The reported method is simple and easy to operate. Self-grafted Arabidopsis – Arabidopsis and Eutrema – Eutrema plants were used as controls, which were obtained with an improved hypocotyl-cutting grafting method. Ion contents in grafted plants were detected by inductively coupled plasma optical emission spectroscopy. The results showed that the ion content in salt cress and Arabidopsis changed to different degrees after grafting. Conclusions The inter-species grafting technique described here makes it possible to study hybrid plants between Arabidopsis and Eutrema and will contribute to further understanding of long-distance communications in plants. This technique also provides a reference for improving plant varieties using grafting, such as gardening plants, as well as fruit and vegetable crops.

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A pin-fasten grafting method provides a non-sterile and highly efficient method for grafting Arabidopsis at diverse developmental stages

Cited by 20 publications

References 31 publications

Micrografting device for testing systemic signaling in Arabidopsis

Micrografting device for testing systemic signaling in Arabidopsis

Plant grafting: insights into tissue regeneration

Methods for grafting Arabidopsis thaliana and Eutrema salsugineum

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