Characterization of<i>Rolled and Erect Leaf 1</i>in regulating leave morphology in rice

Chen, Qiaoling; Xie, Qingjun; Gao, Ju; Wang, Wenyi; Sun, Bo; Liu, Bohan; Zhu, Haitao; Peng, Haifeng; Zhao, Huandong; Liu, Changhong; Wang, Jiang; Zhang, Jing-Liu; Zhang, Guiquan; Zhang, Zemin

doi:10.1093/jxb/erv319

Cited by 55 publications

(44 citation statements)

References 33 publications

(68 reference statements)

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“…To identify the subcellular localization of AL1, A. tumefaciens strains harboring each of the following plasmids separately, pCAMBIA1300-35S-AL1-GFP and pt-rb CD3-1000, were used to transiently coexpress in the rice protoplast as described previously (Zhang et al, 2011), and then the subcellular localization of AL1 and pt-rb CD3-1000 was investigated as described previously (Chen et al, 2015).…”

Section: Confocal Microscopymentioning

confidence: 99%

Albino Leaf 1 that Encodes the Sole Octotricopeptide Repeat Protein Is Responsible for Chloroplast Development in Rice

et al. 2016

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Chloroplast, the photosynthetic organelle in plants, plays a crucial role in plant development and growth through manipulating the capacity of photosynthesis. However, the regulatory mechanism of chloroplast development still remains elusive. Here, we characterized a mutant with defective chloroplasts in rice (Oryza sativa), termed albino leaf1 (al1), which exhibits a distinct albino phenotype in leaves, eventually leading to al1 seedling lethality. Electronic microscopy observation demonstrated that the number of thylakoids was reduced and the structure of thylakoids was disrupted in the al1 mutant during rice development, which eventually led to the breakdown of chloroplast. Molecular cloning revealed that AL1 encodes the sole octotricopeptide repeat protein (RAP) in rice. Genetic complementation of Arabidopsis (Arabidopsis thaliana) rap mutants indicated that the AL1 protein is a functional RAP. Further analysis illustrated that three transcript variants were present in the AL1 gene, and the altered splices occurred at the 39 untranslated region of the AL1 transcript. In addition, our results also indicate that disruption of the AL1 gene results in an altered expression of chloroplast-associated genes. Consistently, proteomic analysis demonstrated that the abundance of photosynthesis-associated proteins is altered significantly, as is that of a group of metabolism-associated proteins. More specifically, we found that the loss of AL1 resulted in altered abundances of ribosomal proteins, suggesting that RAP likely also regulates the homeostasis of ribosomal proteins in rice in addition to the ribosomal RNA. Taken together, we propose that AL1, particularly the AL1a and AL1c isoforms, plays an essential role in chloroplast development in rice.

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Section: Confocal Microscopymentioning

confidence: 99%

Albino Leaf 1 that Encodes the Sole Octotricopeptide Repeat Protein Is Responsible for Chloroplast Development in Rice

et al. 2016

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“…The known loci/genes for the rolled leaf trait are as follows (some genes for abaxial rolling are underlined): qRL-1 [32], url1(t) [33], rl-4 ( rl-2 )[34], SCL1 [35], nal10 [36], REL1 [37], rl8(t) [38], qRL-2-1b [32], CFL1 [39], DNAL1 [40], rl(t) [41], OsAGO1a ( LOC_Os02g45070 )[42], Roc5 [43], Nrl3(t) [44], s1-145 [45], nal7 ( Os03g0162000 )[46], NRL2(t) [47], RL3(t) [44], OsAGO7 [48], rl-3 ( rl-5 )[34], ACL1 [49], SRL2 [50], rl11(t) [51], nl(t) [52], qRL4-2 [22], QFl4 and QFl5 [53], qRL5-9 and qRL5-10 [22], rl8 [54], RL13 [55], qRL-6 [32], sd-sl [56], rl11(t) [51], SRL1 [57], YABBY1 [8], rfs [34], qRL-7 [32], Nul1 [58], QFl7 [53], sll2 [59], qRL-8-1 , qRL-8-2 and qRL-9 [32], OsMYB103L [60], rl13(t) [55], Rl16(t) [61], RL10(t) [62], SLL1 [63], RL9 ( rl9(t) , GARP )[64], OSZHD1 [65], LRL1 [66], QFl9 [53], OSJNBa0003P07 [67], RL12(t) [68], rl15(t) [69], qRL-10 [32], DTL1 [70], RL14 [71], SRS5 [72], NRL1 ( OSJNBa0027H05 )[73], rl-1 ( rl-3 )[34], nal3(t) [74<...>…”

Section: Resultsmentioning

confidence: 99%

Joint Mapping and Allele Mining of the Rolled Leaf Trait in Rice (Oryza sativa L.)

et al. 2016

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The rolled leaf trait, long considered to be a key component of plant architecture, represents an important target trait for improving plant architecture at the population level. We therefore performed linkage mapping using a set of 262 highly variable RILs from two rice cultivars (Minghui 63 and 02428) with minor differences in leaf rolling index (LRI) in conjunction with GWAS mapping of a random subset of the 1127 germplasms from the 3K Rice Genomes Project (3K Rice). A total of seven main-effect loci were found to underlie the transgressive segregation of progenies from parents with minor differences in LRI. Five of these loci were previously identified and two (qRl7b and qRl9b) are newly reported with additional evidence from GWAS mapping for qRl7b. A total of 18 QTLs were identified by GWAS, including four newly identified QTLs. Six QTLs were confirmed by linkage mapping with the above RIL population, and 83.3% were found to be consistent with previously reported loci based on comparative mapping. We also performed allele mining with representative SNPs and identified the elite germplasms for the improvement of rolled leaf trait. Most favorable alleles at the detected loci were contributed by various 3K Rice germplasms. By a re-scanning of the candidate region with more saturated SNP markers, we dissected the region harboring gRl4-2 into three subregions, in which the average effect on LRI was 3.5% with a range from 2.4 to 4.1% in the third subregion, suggesting the presence of a new locus or loci within this region. The representative SNPs for favorable alleles in the reliable QTLs which were consistently identified in both bi-parental mapping and GWAS, such as qRl4, qRl5, qRl6, qRl7a, and qRl7b will be useful for future molecular breeding programs for ideal plant type in rice.

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“…Genes GRAS19 (Chen et al, 2013), LAZY1 (Li et al, 2007), CYP51G3 (Xia et al, 2015), and GSR1 ) present high expression levels at the lamina joint, validating their roles in lamina joint development. In addition, genes involved in the biosynthesis and signaling of BR and auxin, which have been implicated in important roles in the leaf angle, present diverse expression patterns, including decreased expression (CYP51G3 and CYCU4:1), increased expression (DLT [Tong et al, 2009] and GRAS19 and GH3-2 [Tong et al, 2012]), constant high expression levels (LIC and LAZY1 [Zhang et al, 2012]), and constant low expression levels (ILI1 [Zhang et al, 2009a] and REL1 [Chen et al, 2015a]), suggesting the complex regulation of leaf angles via different pathways.…”

Section: Cytological Observation Reveals the Distinct Characteristicsmentioning

confidence: 99%

Dynamic Cytology and Transcriptional Regulation of Rice Lamina Joint Development

2017

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Rice (Oryza sativa) leaf angle is determined by lamina joint and is an important agricultural trait determining leaf erectness and, hence, the photosynthesis efficiency and grain yield. Genetic studies reveal a complex regulatory network of lamina joint development; however, the morphological changes, cytological transitions, and underlying transcriptional programming remain to be elucidated. A systemic morphological and cytological study reveals a dynamic developmental process and suggests a common but distinct regulation of the lamina joint. Successive and sequential cell division and expansion, cell wall thickening, and programmed cell death at the adaxial or abaxial sides form the cytological basis of the lamina joint, and the increased leaf angle results from the asymmetric cell proliferation and elongation. Analysis of the gene expression profiles at four distinct developmental stages ranging from initiation to senescence showed that genes related to cell division and growth, hormone synthesis and signaling, transcription (transcription factors), and protein phosphorylation (protein kinases) exhibit distinct spatiotemporal patterns during lamina joint development. Phytohormones play crucial roles by promoting cell differentiation and growth at early stages or regulating the maturation and senescence at later stages, which is consistent with the quantitative analysis of hormones at different stages. Further comparison with the gene expression profile of leaf inclination1, a mutant with decreased auxin and increased leaf angle, indicates the coordinated effects of hormones in regulating lamina joint. These results reveal a dynamic cytology of rice lamina joint that is fine-regulated by multiple factors, providing informative clues for illustrating the regulatory mechanisms of leaf angle and plant architecture.

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Characterization ofRolled and Erect Leaf 1in regulating leave morphology in rice

Abstract: HighlightThe Rolled and Erect Leaf 1 (REL1) gene is a novel component controlling brassinosteroid signalling-associated leaf morphogenesis and leaf angle in Oryza sativa.

Cited by 55 publications

References 33 publications

Albino Leaf 1 that Encodes the Sole Octotricopeptide Repeat Protein Is Responsible for Chloroplast Development in Rice

Albino Leaf 1 that Encodes the Sole Octotricopeptide Repeat Protein Is Responsible for Chloroplast Development in Rice

Joint Mapping and Allele Mining of the Rolled Leaf Trait in Rice (Oryza sativa L.)

Dynamic Cytology and Transcriptional Regulation of Rice Lamina Joint Development

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