Detection of Mycobacterium avium subspecies in the gut associated lymphoid tissue of slaughtered rabbits

Arrazuria, Rakel; Sevilla, Iker A.; Molina, Elena; Pérez, Valentín Pérez; Garrido, Joseba M.; Juste, Ramón A.; Elguezabal, Natalia

doi:10.1186/s12917-015-0445-2

Cited by 15 publications

(14 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For tissues, brief modifications described by Arrazuria et al (2015) were performed. In both cases, extracted DNA was stored at −20°C for PCR analysis.…”

mentioning

confidence: 99%

Effect of various dietary regimens on oral challenge with Mycobacterium avium subsp. paratuberculosis in a rabbit model

Arrazuria

Molina

Mateo-Abad

et al. 2015

Research in Veterinary Science

Self Cite

View full text Add to dashboard Cite

“…For tissues, brief modifications described by Arrazuria et al (2015) were performed. In both cases, extracted DNA was stored at −20°C for PCR analysis.…”

mentioning

confidence: 99%

Effect of various dietary regimens on oral challenge with Mycobacterium avium subsp. paratuberculosis in a rabbit model

Arrazuria

Molina

Mateo-Abad

et al. 2015

Research in Veterinary Science

Self Cite

View full text Add to dashboard Cite

“…In humans, NTM cause an important infectious disease, and the main risk factor is thought to be environmental exposure. Although an increasing number of studies are targeting the prevalence and distribution of NTM (Gcebe et al, 2013;Arrazuria et al, 2015), there has been only limited research on the host defense mechanisms against NTM.…”

Section: Introductionmentioning

confidence: 99%

Prominent contribution of Th1, Th17, and Tregs to the host response during M. neoaurum infection

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. Nontuberculous mycobacteria are ubiquitous in outside environment and animals. As for nontuberculous mycobacteria infection, there is only limited information in humans regarding infection and the subsequent immune response, especially for Mycobacterium neoaurum. Here, haematoxylin-eosin and Ziehl-Neelsen staining were used to observe pathological changes and detect acid-fast bacilli in organ samples in mouse model. tissues. Our results indicated that 72% of CD4+ T cells appeared in the early days of infection, which was followed by a decrease to 47% by day 32, and then a rise to 76% by day 56. Moreover, we found higher frequency of IFN-g-producing CD4+ T cells and elevated mRNA expression of the transcription factor T-bet in the lungs; however, we observed lower mRNA expression of the transcription factor RORgt and lower frequency of IL-17-producing CD4+ T cells. A transient relative decrease in the number of Treg cells was observed in the lungs; however, the number of Tregs did not change significantly between the first and last day following infection. Thus, M. neoaurum causes chronic infection in C57BL/6 mice, with Th1, Th17, and Tregs playing a prominent role in the host response. The present study may lay the basis for further studies on the mechanisms underlying infection with nontuberculous mycobacteria.

show abstract

“…More recently in a survey carried out on slaughtered rabbits in northern Spain, Maa was detected by PCR in gut associated lymphoid tissue of 16.6% of the analysed animals; however, no isolation was achieved from any of the slaughtered rabbits (Arrazuria et al., ).…”

Section: Infection By Non‐tuberculous Mycobacteriamentioning

confidence: 99%

Mycobacterial Infections in Rabbits: From the Wild to the Laboratory

Arrazuria

Juste

Elguezabal

2016

Transbound Emerg Dis

View full text Add to dashboard Cite

Tuberculous mycobacterial diseases such as leprosy and tuberculosis are ancient diseases that currently continue threatening human health in some countries. Non-tuberculous mycobacterial (NTM) infections cause a series of well-defined pathological entities, as well as some opportunistic diseases that have also increased worldwide, being more common among immunocompromised patients but rising also in immunocompetent individuals. Reports on natural infections by mycobacteria in rabbits are scarce and mainly involve NTM such as Mycobacterium avium subsp. avium in pigmy rabbits in the United States and Mycobacterium avium subsp. paratuberculosis in wild rabbits in Europe. Rabbits have been used as laboratory animals through the years, both to generate immunological reagents and as infection models. Mycobacterial infection models have been developed in this animal species showing different susceptibility patterns to mycobacteria in laboratory conditions. The latent tuberculosis model and the cavitary tuberculosis model have been widely used to elucidate pathogenic mechanisms and to evaluate chemotherapy and vaccination strategies. Rabbits have also been used as bovine paratuberculosis infection models. This review aimed to gather both wildlife and experimental infection data on mycobacteriosis in rabbits to assess their role in the spread of these infections as well as their potential use in the experimental study of mycobacterial pathogenesis and treatment.

show abstract

Detection of Mycobacterium avium subspecies in the gut associated lymphoid tissue of slaughtered rabbits

Cited by 15 publications

References 41 publications

Effect of various dietary regimens on oral challenge with Mycobacterium avium subsp. paratuberculosis in a rabbit model

Effect of various dietary regimens on oral challenge with Mycobacterium avium subsp. paratuberculosis in a rabbit model

Prominent contribution of Th1, Th17, and Tregs to the host response during M. neoaurum infection

Mycobacterial Infections in Rabbits: From the Wild to the Laboratory

Contact Info

Product

Resources

About