Randomised clinical study: <i>Aspergillus niger</i>‐derived enzyme digests gluten in the stomach of healthy volunteers

Salden, Bouke; Monserrat, V.; Troost, Freddy J.; Bruins, Maaike J.; Edens, Luppo; Bartholomé, Roger; Haenen, Guido R.M.M.; Winkens, Björn; Koning, Frits; Masclee, Ad

doi:10.1111/apt.13266

Cited by 61 publications

(45 citation statements)

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“…For instance, the use of prolyl endopeptidase (PEP) enzymes as a tool to detoxify gluten fragments and cleave peptides at proline or glutamine residues has been proposed. ALV003 and AN‐PEP from Aspergillus niger (AN‐PEP) are currently under clinical investigation and preliminary results demonstrated that they are well tolerated by CD patients . However, the major problem related to their use is connected with their stability in acidic gastric conditions and their efficiency in gluten degradation.…”

Section: Discussionmentioning

confidence: 99%

Lack of immunogenicity of hydrolysed wheat flour in patients with coeliac disease after a short‐term oral challenge

Mandile

Picascia

Parrella

et al. 2017

Aliment Pharmacol Ther

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Section: Discussionmentioning

confidence: 99%

Lack of immunogenicity of hydrolysed wheat flour in patients with coeliac disease after a short‐term oral challenge

Mandile

Picascia

Parrella

et al. 2017

Aliment Pharmacol Ther

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“…This non-validated questionnaire has been described before by Salden et al . [17]. Feelings of abdominal discomfort, abdominal pain, abdominal distension, constipation, diarrhea, flatulence, eructation, nausea and total discomfort were assessed by scores from 1 (no symptoms) to 5 (pronounced symptoms).…”

Section: Methodsmentioning

confidence: 99%

Effects of Supplementation of the Synbiotic Ecologic® 825/FOS P6 on Intestinal Barrier Function in Healthy Humans: A Randomized Controlled Trial

et al. 2016

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Background and AimsProbiotics, prebiotics and synbiotics have been suggested as dietary strategies to improve intestinal barrier function. This study aimed to assess the effect of two weeks synbiotic supplementation on intestinal permeability under basal and stressed conditions. Secondary aims were the assessment of two weeks synbiotic supplementation on systemic immune function and gastrointestinal symptoms including defecation pattern.DesignTwenty healthy adults completed a double-blind, controlled, randomized, parallel design study.InterventionGroups either received synbiotic (1.5 × 1010 CFU Ecologic® 825 + 10 g fructo-oligosaccharides (FOS P6) per day) or control supplements for two weeks.OutcomesIntestinal segment specific permeability was assessed non-invasively by oral administration of multiple sugar probes and, subsequently, assessing the excretion of these probes in urine. This test was conducted at baseline and at the end of intervention, in the absence and in the presence of an indomethacin challenge. Indomethacin was applied to induce a compromised gut state. Plasma zonulin, cytokines and chemokines were measured at baseline and at the end of intervention. Gastrointestinal symptoms and stool frequency were recorded at baseline and daily during intervention.ResultsSignificantly more male subjects were in the synbiotic group compared to the control group (P = 0.025). Indomethacin significantly increased urinary lactulose/rhamnose ratio versus without indomethacin, both in the control group (P = 0.005) and in the synbiotic group (P = 0.017). Urinary sugar recoveries and ratios, plasma levels of zonulin, cytokines and chemokines, and gastrointestinal symptom scores were not significantly different after control or synbiotic intervention. Stool frequency within the synbiotic group was significantly increased during synbiotic intervention compared to baseline (P = 0.039) and higher compared to control intervention (P = 0.045).ConclusionTwo weeks Ecologic® 825/FOS P6 supplementation increased stool frequency, but did not affect intestinal permeability neither under basal nor under indomethacin-induced stressed conditions, immune function or gastrointestinal symptoms in healthy adults.

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“…Consequently, it is capable of degrading gluten in the stomach compartment both in vitro and in vivo and it has been found to exert no harmful effects in patients and healthy human volunteers [32,[35][36][37] . The enzyme is expected to reach the market as an over-the-counter product in 2015.…”

Section: Enzymatic Gluten Degradationmentioning

confidence: 99%

Adverse Effects of Wheat Gluten

Koning¹

2015

Ann Nutr Metab

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Man began to consume cereals approximately 10,000 years ago when hunter-gatherers settled in the fertile golden crescent in the Middle East. Gluten has been an integral part of the Western type of diet ever since, and wheat consumption is also common in the Middle East, parts of India and China as well as Australia and Africa. In fact, the food supply in the world heavily depends on the availability of cereal-based food products, with wheat being one of the largest crops in the world. Part of this is due to the unique properties of wheat gluten, which has a high nutritional value and is crucial for the preparation of high-quality dough. In the last 10 years, however, wheat and gluten have received much negative attention. Many believe that it is inherently bad for our health and try to avoid consumption of gluten-containing cereals; a gluten-low lifestyle so to speak. This is fueled by a series of popular publications like Wheat Belly; Lose the Wheat, Lose the Weight, and Find Your Path Back to Health. However, in reality, there is only one condition where gluten is definitively the culprit: celiac disease (CD), affecting approximately 1% of the population in the Western world. Here, I describe the complexity of the cereals from which gluten is derived, the special properties of gluten which make it so widely used in the food industry, the basis for its toxicity in CD patients and the potential for the development of safe gluten and alternatives to the gluten-free diet.

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Randomised clinical study: Aspergillus niger‐derived enzyme digests gluten in the stomach of healthy volunteers

Cited by 61 publications

References 37 publications

Lack of immunogenicity of hydrolysed wheat flour in patients with coeliac disease after a short‐term oral challenge

Lack of immunogenicity of hydrolysed wheat flour in patients with coeliac disease after a short‐term oral challenge

Effects of Supplementation of the Synbiotic Ecologic® 825/FOS P6 on Intestinal Barrier Function in Healthy Humans: A Randomized Controlled Trial

Adverse Effects of Wheat Gluten

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