Corrigendum: Single cell electroporation for longitudinal imaging of synaptic structure and function in the adult mouse neocortex in vivo

ePages, Stephane; eCane, Michele; eRandall, Jerome; eCapello, Luca; eHoltmaat, Anthony

doi:10.3389/fnana.2015.00056

Cited by 2 publications

(3 citation statements)

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“…In vivo -targeted electroporation was performed on single tectal neurons within the albino Xenopus laevis tadpole using a pipette electrode filled with a solution of the delivery molecules and with a tip diameter much smaller than the width of the target cell [114]. A similar method was utilized for longitudinal imaging of synaptic structure and function in the adult mouse neocortex in vivo [115].…”

Section: Micropipette Based Scep and Configurationsmentioning

confidence: 99%

Single-cell electroporation: current trends, applications and future prospects

Kar

Mohan

Dey

et al. 2018

J. Micromech. Microeng.

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The ability to deliver foreign molecules into a single living cell with high transfection efficiency and high cell viability is of great interest in cell biology for applications in therapeutic development, diagnostics and drug delivery towards personalized medicine. Many chemical and physical methods have been developed for cellular delivery, however most of these techniques are bulk approach, which are cell-specific and have low throughput delivery. On the other hand, electroporation is an efficient and fast method to deliver exogenous biomolecules such as DNA, RNA and oligonucleotides into target living cells with the advantages of easy operation, controllable electrical parameters and avoidance of toxicity. The rapid development of micro/nanofluidic technologies in the last two decades, enables us to focus an intense electric field on the targeted cell membrane to perform single cell micro-nano-electroporation with high throughput intracellular delivery, high transfection efficiency and cell viability. This review article will emphasize the basic concept and working mechanism associated with electroporation, single cell electroporation and biomolecular delivery using micro/nanoscale electroporation devices, their fabrication, working principles and cellular analysis with their advantages, limitations, potential applications and future prospects.

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Section: Micropipette Based Scep and Configurationsmentioning

confidence: 99%

Single-cell electroporation: current trends, applications and future prospects

Kar

Mohan

Dey

et al. 2018

J. Micromech. Microeng.

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“…Dendritic spine imaging can be performed on primary neuronal culture, organotypic neuronal cultures, acute brain slices, in adult animals during live cell imaging or after chemical cell fixation (see Table 1 and Table 2 ). Live imaging, in vitro, and in vivo, is the most relevant approach to study the mechanisms underlying spine structure [ 16 , 18 , 21 , 24 , 25 , 26 , 28 , 38 , 44 , 45 , 70 , 94 ], however, to determine global changes in structural remodeling, the spine analysis after culture/tissue fixation enables to analyze larger number of spines. For in vitro imaging, primary neuronal cultures are mostly used, in which dendritic morphogenesis develops during first two weeks [ 95 ] and thereafter spines start to maturate.…”

Section: Experimental Methodologymentioning

confidence: 99%

“…Therefore, the live cell imaging approach constitutes a suitable alternative, however, it requires analyzing more neurons, culture or animals per group due to technical limitations during live imaging. Imaging in vivo is usually performed in animals under anesthesia [ 38 , 94 ], sensory experience [ 27 ], or after behavioral training [ 29 , 45 , 47 ]. For in vivo live cell imaging, transgenic mice expressing fluorescent proteins (GFP/RFP/YFP) within whole brain, brain regions, or specific cell types are used [ 38 , 40 , 41 , 45 , 103 ].…”

Section: Experimental Methodologymentioning

confidence: 99%

Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions

Bączyńska

Pels

Basu

et al. 2021

IJMS

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Numerous brain diseases are associated with abnormalities in morphology and density of dendritic spines, small membranous protrusions whose structural geometry correlates with the strength of synaptic connections. Thus, the quantitative analysis of dendritic spines remodeling in microscopic images is one of the key elements towards understanding mechanisms of structural neuronal plasticity and bases of brain pathology. In the following article, we review experimental approaches designed to assess quantitative features of dendritic spines under physiological stimuli and in pathological conditions. We compare various methodological pipelines of biological models, sample preparation, data analysis, image acquisition, sample size, and statistical analysis. The methodology and results of relevant experiments are systematically summarized in a tabular form. In particular, we focus on quantitative data regarding the number of animals, cells, dendritic spines, types of studied parameters, size of observed changes, and their statistical significance.

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Corrigendum: Single cell electroporation for longitudinal imaging of synaptic structure and function in the adult mouse neocortex in vivo

Abstract: A corrigendum on:Single cell electroporation for longitudinal imaging of synaptic structure and function in the adult mouse neocortex in vivo by Pagès, S., Cane, M., Randall, J., Capello, L., and Holtmaat, A. (2015). Front. Neuroanat. 9:36.

Cited by 2 publications

References 0 publications

Single-cell electroporation: current trends, applications and future prospects

Single-cell electroporation: current trends, applications and future prospects

Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions

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