Abiotic stress induces change in Cinnamoyl CoA Reductase (CCR) protein abundance and lignin deposition in developing seedlings of Leucaena leucocephala

Abstract

Aboitic stress such as drought and salinity are class of major threats, which plants undergo through their lifetime. Lignin deposition is one of the responses to such abiotic stresses. The gene encoding Cinnamoyl CoA Reductase (CCR) is a key gene for lignin biosynthesis, which has been shown to be over-expressed under stress conditions. In the present study, developing seedlings of Leucaena leucocephala (Vernacular name: Subabul, White popinac) were treated with 1 % mannitol and 200 mM NaCl to mimic drought and salinity stress conditions, respectively. Enzyme linked immunosorbant assay (ELISA) based expression pattern of CCR protein was monitored coupled with Phlorogucinol/HCl activity staining of lignin in transverse sections of developing L. leucocephala seedlings under stress. Our result suggests a differential lignification pattern in developing root and stem under stress conditions. Increase in lignification was observed in mannitol treated stems and corresponding CCR protein accumulation was also higher than control and salt stress treated samples. On the contrary CCR protein was lower in NaCl treated stems and corresponding lignin deposition was also low. Developing root tissue showed a high level of CCR content and lignin deposition than stem samples under all conditions tested. Overall result suggested that lignin accumulation was not affected much in case of developing root however developing stems were significantly affected under drought and salinity stress condition.

BpNAC012 Positively Regulates Abiotic Stress Responses and Secondary Wall Biosynthesis

“…Several studies have shown that lignin accumulation and high levels of lignin biosynthetic gene expression are important for abiotic stress tolerance (Hu et al, 2009;Shafi et al, 2015;Srivastava et al, 2015). In this study, heavier deposition of lignin was observed specifically in the secondary xylem and phloem fibers of BpNAC012-OE transgenic plants under salt and osmotic stress conditions, which was consistent with the results that BpNAC012 highly induced the expression of lignin biosynthetic genes in response to abiotic stress treatments.…”

Cloning and Phylogenetic Analysis of Brassica napus L. Caffeic Acid O-Methyltransferase 1 Gene Family and Its Expression Pattern under Drought Stress

“…Drought stress had a positive effect on lignin deposition and deepened the lignified degree of developing seedling stems in Leucaena leucocephala [61]. The expression of CAD (cinnamyl alcohol dehydrogenase) and several other genes involving in lignin biosynthesis increased during 48h to 72h under water stress in rice root [62].…”

Paving the Way for Lignin Valorisation: Recent Advances in Bioengineering, Biorefining and Catalysis

“…[5,41,99,[145][146][147][148][149][150][151] The response of these pathways to abiotic stresses is crucial for understanding the full biological role of lignin in the plant. [5,145] Effects of abiotic stress can be analysed either by individually introducing stresses,e .g., growing the plant in amedium with high salinity), [150,152] or by monitoring how the plants perform in ag reenhouse setting vs.i nf ield trials. [153] Recent review literature provides ac omparison of the impacts of biotic and abiotic stress on plant fitness when transferring from ac ontrolled greenhouse environment to field trials.…”