In vitro cytotoxicity of silver nanoparticles and zinc oxide nanoparticles to human epithelial colorectal adenocarcinoma (Caco-2) cells

Song, Yijuan; Guan, Rongfa; Lyu, Fei; Kang, Tian-Shu; Wu, Yicheng; Chen, Xiaoqiang

doi:10.1016/j.mrfmmm.2014.08.001

Cited by 91 publications

(63 citation statements)

References 25 publications

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“…NP treatment results in a noticeable change in cellular shape, or morphology, in vitro [28]. Therefore, we examined cellular morphology by microscopy.…”

Section: 2mentioning

confidence: 99%

“…Also, newly formed products such as hydrozincite (Zn 5 (CO 3 ) 2 (OH) 6 ), smithsonite (ZnCO 3 ), and zinc sulfide (ZnS) are possible in the presence of an appropriate concentration of carbonate and sulfide and under suitable pH conditions [19][20][21][22][23]. An increasing number of studies have shown that ZnO NPs induce various toxic effects, including cytotoxicity, genotoxicity, inflammation, and oxidative stress [12,[24][25][26][27][28]. Among these documented adverse effects, mutagenicity is particularly important because it is inheritable and could therefore cause severe and far-reaching negative effects to human health [29].…”

Section: Introductionmentioning

confidence: 99%

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Natural Transformation of Zinc Oxide Nanoparticles and Their Cytotoxicity and Mutagenicity

Wang

Cao

et al. 2017

Journal of Nanomaterials

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With rapid development of the nanoindustry, studies focusing on the transformation of nanoparticles (NPs) are required to understand their stability and toxicity after being released into the environment. Here, we characterized the physicochemical properties of ZnO NPs and found that they are naturally alkalized in the presence of air (without the addition of exogenous alkaline substances). Energy dispersive X-ray/X-ray powder diffraction/Fourier transform infrared (EDX/XRD/FTIR)/Raman spectroscopy gave evidence for the formation of hydrozincite (Zn 5 (CO 3 ) 2 (OH) 6 ) and zinc hydroxide (Zn(OH) 2 ). Further, we comparatively evaluated the cellular toxicity of pristine and alkalized ZnO NPs. Cell viability testing (colony formation) showed that alkalization time-dependently decreased cytotoxicity. Alkalized NPs exhibited mutagenicity at multiple concentrations, as shown by a CD59 gene loci mutation assay. Variations in toxicity were associated with the chemical transformation of ZnO NPs, and Zn 2+ played a key role in the mutagenicity of alkalized NPs. These results indicate that NPs are chemically transformed in the environment. These transformations result in obvious variations in toxicity, suggesting that the NP transformation process should be considered more thoroughly when evaluating toxicity.

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“…NP treatment results in a noticeable change in cellular shape, or morphology, in vitro [28]. Therefore, we examined cellular morphology by microscopy.…”

Section: 2mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Natural Transformation of Zinc Oxide Nanoparticles and Their Cytotoxicity and Mutagenicity

Wang

Cao

et al. 2017

Journal of Nanomaterials

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“…The in vitro cell viability of human breast cancer cells (MCF-7) exposed to the SPIONs was assessed by using a MTT cytotoxicity assay [33,34]. MCF-7 cells were cultured overnight M a n u s c r i p t 5 in 96-well plates (4 10 3 cells per well) to allow cell attachment, and then incubated with 0.1 ml H-DMEM media containing 0.0, 3.1, 6.2, 12.5, 25.0, 50.0 and 100.0 µg Fe/ml of PEG-SPIONs, PEG/PEI-SPIONs and PEG/PEI/Tween 80-SPIONs for 24 h, respectively.…”

Section: Cytotoxicity Assay In Vitromentioning

confidence: 99%

Superparamagnetic iron oxide nanoparticles coated with different polymers and their MRI contrast effects in the mouse brains

Xie

Zhang

Wang

et al. 2015

Applied Surface Science

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“…17,29 The decrease in metabolic activity is also in agreement with several papers in Caco-2 cells treated with Ag NP. 14,16,17,29,30 Treatment of proliferating cells with Ag NP for 24 hours decreased the GSH/GSSG ratio to less than half the level in untreated cells, indicating oxidative stress (Figure 8). Other studies in Caco-2 cells have shown induction of oxidative stress by detection of ROS species production 17,31 and gene expression studies showed upregulation of genes involved in oxidative stress in the Caco-2/M cell model.…”

Section: Proliferating Cellsmentioning

confidence: 99%

“…15,17,32 Other studies in Caco-2 cells disagree with us and observed toxicity at Ag NP doses lower than those we used. 14,16,29,30 However, it is likely that these differences are due to studies being performed at lower cell densities (between 10,000 and 100,000 cells/cm 2 ) so that cells may be more sensitive to NP treatment 16,30 and differences in the Ag NP used in the studies.…”

Section: Confluent Cellsmentioning

confidence: 99%

Oxidative stress-mediated inhibition of intestinal epithelial cell proliferation by silver nanoparticles

McCracken

Zane

Knight

et al. 2015

Toxicology in Vitro

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Abstract:Use of silver nanoparticles (NP) is of interest in the food and food packaging industries for their antimicrobial properties. In order to investigate potential consequences of Ag NP ingestion, this study was performed in the intestinal epithelial cell line C2BBe1. Ag NP were synthesized and characterized prior to biological experiments. The Ag NP had an average diameter of 23 nm as determined by TEM analysis. treated with up to 10 µg/cm 2 (50 µg/mL) Ag NP, suggesting that these cells are not as sensitive to Ag NP. This is likely due to exposure of each cell to a smaller number of Ag NP when the cells are confluent in addition to decreased metabolic activity and increased resistance to toxic agents in confluent cells. Based on these studies, Ag NP ingestion may be able to slow proliferation of 3 stem cells of the intestinal crypt and this is something that needs to be investigated further. The largest effects may be seen in diseased intestines where the epithelium is compromised. 4Introduction:

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In vitro cytotoxicity of silver nanoparticles and zinc oxide nanoparticles to human epithelial colorectal adenocarcinoma (Caco-2) cells

Cited by 91 publications

References 25 publications

Natural Transformation of Zinc Oxide Nanoparticles and Their Cytotoxicity and Mutagenicity

Natural Transformation of Zinc Oxide Nanoparticles and Their Cytotoxicity and Mutagenicity

Superparamagnetic iron oxide nanoparticles coated with different polymers and their MRI contrast effects in the mouse brains

Oxidative stress-mediated inhibition of intestinal epithelial cell proliferation by silver nanoparticles

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