DNA electrochemical biosensor for metallic drugs at physiological conditions

Abstract

Entrapment of dsSS-DNA into the polypyrrole-polyvinyl sulphonate (dsSS-DNA-PPy-PVS) film over indium-tin-oxide (ITO) coated glass has been designed to detect titanium and platinum drugs, titanocene dichloride and cisplatin. The disposable dsSS-DNA-PPy-PVS/ITO biosensor was characterized by cyclic voltammetry, attenuated total reflectance Infrared spectroscopy and atomic force microscopy. Amperometric studies by cyclic voltammetry using, dsSS-DNA-PPy PVS/ITO biosensor, demonstrated the ability of this biosensor to detect these metallic drugs in millimolar concentration by monitoring the decrease of the guanine oxidation signal as a result of the DNA damage. The concentration range detected for titanocene dichloride is 0.25 to 1.5 mM and for cisplatin is 0.06 to 1.0 mM.

Pt and Ti Complexes Immobilized onto Mesoporous Silica Microspheres and Their Interaction with Molecules of Biological Interest

“…Traditionally, research on surface-modified electrodes has involved studies of catalysis and inhibition of various electrochemical processes, the electrochemistry of the attached mol-ecules and applications such as photo-electrodes or analytical determinations. [21,22] However, to date, only a few reports are available dealing with the electrochemical response of redox moieties covalently attached to the walls of mesoporous silica. [23] In this section, differential pulse voltammetry measurements have been performed to test the electro-activity of the titanium and platinum complexes tethered onto MSMs.…”

A Reduced Graphene Oxide‐based Electrochemical DNA Biosensor for the Detection of Interaction between Cisplatin and DNA based on Guanine and Adenine Oxidation Signals

“…Because G−N7 is the most electron‐rich site on dsDNA, it is preferentially involved in reacting with cisplatin , , . The interaction of cisplatin with dsDNA can readily be studied by electroanalytical methods based on the decrease in the guanine and adenine oxidation signals , .…”

“…EC provides direct qualitative and quantitative informations on the interaction between redox active species and DNA fragments. There has been a great interest for the design of DNA or oligonucleotide immobilized electrodes in order to investigate electrochemically the interaction between DNA and anticancer drugs .…”

“…Two methods have been reported showing the utility of graphene‐based electrodes for the electrochemical determination of cisplatin and related compounds , . There have been several reports dealing with the monitoring of cisplatin and derivatives interaction at DNA modified electrodes but, and to the best of our knowledge, no literature reports were found devoted to the monitoring of cisplatin‐DNA interaction at a DNA modified graphene‐based electrode.…”

Optical Biosensors for Therapeutic Drug Monitoring

“…Amperometric biosensors measure current flows generated by an electrochemical reaction at a constant potential, where the current intensity is directly related to the concentration of the substance oxidized and reduced on the electrode's surface [63]. These types of biosensors have been used in quantifying aminoglycoside antibiotics [66], bronchodilators (such as theophylline) [67], anti-arrhythmic [68], and anticancer drugs [69][70][71][72][73][74][75][76][77][78][79][80][81][82][83].…”