Origin of the dengue virus outbreak in Martin County, Florida, USA 2013

Teets, Frank; Ramgopal, Moti; Sweeney, Kristen; Graham, Amanda S.; Michael, Scott F.; Isern, Sharon

doi:10.1016/j.virep.2014.05.001

Cited by 34 publications

(27 citation statements)

References 13 publications

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“…The 2013 DENV outbreak in Florida was attributed to introduction of DENV-1, [8] as our PRNT results also confirmed. The infected individuals were previously naive to DENV, as supported by overall low titers and low cross-reactivity to other serotypes [12].…”

Section: Discussionsupporting

confidence: 78%

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Dengue Outbreak in Martin County, Florida in 2013

M¹

2015

JHVRV

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Following a 2013 outbreak of dengue serotype 1 in Martin County, Florida, plasma samples from individuals with suspected dengue illness were tested for serotypespecific neutralization of dengue viruses 1-4. Titers and seroprevalence in these suspected cases from Florida were compared to those from (1) healthy donors from the U.S. and (2) dengue-suspected and dengue-confirmed samples collected from dengue-endemic regions during outbreaks in Puerto Rico in 2010 and Brazil in 2014. By dengue plaque reduction neutralization titer assays, we confirmed that four of the nine suspected individuals from Martin County Florida had been exposed to dengue virus serotype 1, and that these were primary dengue viral infections. Furthermore, primary and secondary dengue viral infections were observed in the Brazilian cohort, and a high incidence of secondary infections was observed in the Puerto Rican samples. Routine serotype-specific surveillance may be needed to continue monitoring establishment of dengue serotype 1 and introduction of other serotypes in Florida.

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Section: Discussionsupporting

confidence: 78%

“…There were 4 asymptomatic, but laboratory-confirmed DENV positive individuals. The strains isolated in the Martin County outbreak originated from a DENV-1 circulating in the Caribbean from South American origins, while the 2010 Key West outbreak strain was most closely related to Central American viruses [8].…”

Section: Introductionmentioning

confidence: 99%

Dengue Outbreak in Martin County, Florida in 2013

M¹

2015

JHVRV

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“…The incidence is even higher in tropical and subtropical countries where environmental conditions favor the proliferation of the Aedes mosquitoes [2–5]. However, this disease is also advancing into developed countries, where the occurrence of dengue has not been previously registered, such as the United States of America [6–8], Japan [9–11], and France [12]. …”

Section: Introductionmentioning

confidence: 99%

Low sensitivity of the tourniquet test for differential diagnosis of dengue: an analysis of 28,000 trials in patients

et al. 2016

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BackgroundThe aim of this study was to evaluate the utility of the tourniquet test (TT) for dengue diagnosing. To our knowledge, no previous study with such a large sample, of this duration, with as many laboratory methods referenced, or relating the results of the TT to the 2009 WHO classification of severity has been conducted thus far.MethodsIn this study, we analyzed the records of 119,589 suspected dengue cases in a Brazilian city, with 30,670 confirmed cases. The Cohen’s Kappa test was applied to evaluate the degree of agreement between the tests, and the sensitivity and specificity was calculated for the TT.ResultsTwenty-eight thousand six hundred thirty-five TT were performed. No association between the outcome of the TT and greater severity of infection, according to the 2009 guideline, was observed (P = 0.28); furthermore, relevant agreement with the final diagnosis (κ = 0.01; 95 % CI = 0.00 to 0.02) or individually with the IgM enzyme-linked immunoassay was not observed (κ = 0.05; 95 % CI = 0.04 to 0.06), and was even lower with PCR (κ = 0.27; 95 % CI = 0.06 to 0.49). Most importance of the TT was shown in relation to specificity (88.9 %; 95 % CI = 0.88 to 0.89) and negative predictive value (70.3 %; CI 95 % = 0.70 to 0.71).ConclusionsTT was more effective in detecting cases that were truly negative than positive. These results suggest that the TT should not be used as diagnosis of dengue.

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“…aegypti, which is constrained by its affinity for tropical and sub-tropical climate [12]. However, there have been instances of seasonal transmission of DENV, CHIKV, and ZIKV in sub-tropical and even temperate areas [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Investigating the probability of establishment of Zika virus and detection through mosquito surveillance under different temperature conditions

Christofferson

2018

Preprint

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words)Because of the increasing threat that Zika virus (ZIKV) poses to extra-tropical regions due to increased global travel, there is a need for better understanding of the effect(s) of temperature on the establishment potential of ZIKV within these subtropical, temperate, and/or seasonal Ae. aegypti populations. The first step to determining risk establishment of ZIKV in these regions is to assess ZIKV's ability to infect mosquitoes at less tropical temperatures, and thus be detected through common surveillance programs. To that end, the effect of two rearing temperatures (RT) and extrinsic incubation temperatures (EIT) on infection and dissemination rates was evaluated, as well as the interactions of such. Total, there were four combinations (RT24-EIT24, RT24-DEI28, RT28-EIT24, RT28-EIT28). Further, a stochastic SEIR framework was adapted to determine whether observed data could lead to differential success of establishment of ZIKV in naive mosquito populations. There was no consistent pattern in significant differences found across treatments for either infection or dissemination rates (p>0.05), where only a significant difference was found in infection rates between RT24-EIT24 (44%) and RT28-EIT24(82.6%). Across all temperature conditions, the model predicted between a 77.3% and 93.1% chance of successful establishment of ZIKV in naive mosquito populations under model assumptions. Further, the model predicted between 4.1% and 46.7% chance of at least one mosquito developing a disseminated infection, depending on temperature conditions, despite no significant differences in the experimental data. These results indicate that 1) there is no straightforward relationship between RT, EIT, and infection/dissemination rates for ZIKV, similar to what has been reported for DENV, 2) in more temperate climates, ZIKV may still have the ability to establish in populations of Ae. aegypti, and 3) despite a lack of statistical differences in observed experimental data, model predictions indicate that the interplay of rearing and extrinsic incubation temperatures may still alter the kinetics of ZIKV within the mosquito enough to affect numbers of infected/disseminated mosquitoes and the associated probability of detection through surveillance programs.

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Origin of the dengue virus outbreak in Martin County, Florida, USA 2013

Cited by 34 publications

References 13 publications

Dengue Outbreak in Martin County, Florida in 2013

Dengue Outbreak in Martin County, Florida in 2013

Low sensitivity of the tourniquet test for differential diagnosis of dengue: an analysis of 28,000 trials in patients

Investigating the probability of establishment of Zika virus and detection through mosquito surveillance under different temperature conditions

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