Endothelial Cells Control Pancreatic Cell Fate at Defined Stages through EGFL7 Signaling

Kao, Der-I; Lacko, Lauretta A.; Ding, Bi-Sen; Huang, Chen; Phung, Kathleen; Gu, Guoqiang; Rafii, Shahin; Stuhlmann, Heidi; Chen, Shuibing

doi:10.1016/j.stemcr.2014.12.008

Cited by 40 publications

(29 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The vasculature can secrete specific factors at the sites of tissue injury that can influence tissue regeneration, as seen in the lung (66) and liver (67). This effect has also been noted in various tissues, including: pancreatic islet cells (68), adipose stem cells (69), bone (70, 71) and cardiac endothelium (72). Beginning in 2000, Rafii and other groups showed that it was possible to produce organ-specific endothelial cells (73–76).…”

Section: Introductionmentioning

confidence: 82%

Understanding the Pathophysiology and Challenges of Development of Medical Countermeasures for Radiation-Induced Vascular/Endothelial Cell Injuries: Report of a NIAID Workshop, August 20, 2015

2016

View full text Add to dashboard Cite

After the events of September 11, 2001, a decade of research on the development of medical countermeasures (MCMs) to treat victims of a radiological incident has yielded two FDA-approved agents to mitigate acute radiation syndrome. These licensed agents specifically target the mitigation of radiation-induced neutropenia and infection potential, while the ramifications of the exposure event in a public health emergency incident could include the entire body, causing additional acute and/or delayed organ/tissue injuries. Anecdotal data as well as recent findings from both radiation accident survivors and animal experiments implicate radiation-induced injury or dysfunction of the vascular endothelium leading to tissue and organ injuries. There are significant gaps in our understanding of the disease processes and progression, as well as the optimum approaches to develop medical countermeasures to mitigate radiation vascular injury. To address this issue, the Radiation and Nuclear Countermeasures Program of the National Institute of Allergy and Infectious Diseases (NIAID) organized a one-day workshop to examine the current state of the science in radiation-induced vascular injuries and organ dysfunction, the natural history of the pathophysiology and the product development maturity of potential medical countermeasures to treat these injuries. Meeting presentations were followed by a NIAID-led open discussion among academic investigators, industry researchers and government agency representatives. This article provides a summary of these presentations and subsequent discussion from the workshop.

show abstract

Section: Introductionmentioning

confidence: 82%

Understanding the Pathophysiology and Challenges of Development of Medical Countermeasures for Radiation-Induced Vascular/Endothelial Cell Injuries: Report of a NIAID Workshop, August 20, 2015

2016

View full text Add to dashboard Cite

show abstract

“…Angiocrine signals from ECs also guide the stage-specific differentiation of pluripotent stem cells into islet cells. For example, EC-derived EGF-like 7 (EGFL7) specifies the fate and proliferation of pancreas and duodenum homeobox (Pdx)1-positive islet-cell progenitors 100 . Therefore, identification of the phenotypic and functional attributes of islet-specific ECs could unravel the angiocrine heterogeneity within pancreatic tissues and lead to new therapeutic strategies for islet-cell production.…”

Section: The Modulation Of Metabolismmentioning

confidence: 99%

Angiocrine functions of organ-specific endothelial cells

Rafii

Butler

Ding

2016

Nature

Self Cite

755

647

View full text Add to dashboard Cite

Preface Endothelial cells lining blood vessel capillaries are not just passive conduits for delivering blood. Tissue-specific endothelium establish specialized vascular niches that deploy specific sets of growth factors, known as angiocrine factors, which actively participate in inducing, specifying, patterning, and guiding organ regeneration and maintaining homeostasis and metabolism. Angiocrine factors upregulated in response to injury orchestrates self-renewal and differentiation of tissue-specific repopulating resident stem and progenitor cells into functional organs. Uncovering the precise mechanisms whereby physiological-levels of angiocrine factors are spatially and temporally produced, and distributed by organotypic endothelium to repopulating cells, will lay the foundation for driving organ repair without scarring.

show abstract

“…41,42 Once they reached B80-90% confluency they were treated for one day with 3 mm CHIR99021 (StemRD) and 100 ng mL À1 activin A (R&D systems) in RPMI medium (Cellgro) supplemented with 2 mM GlutaMAX (Gibco) and 100 U mL À1 Pen/Strep (Gibco). For the following two days the cells were treated with 100 ng mL À1 activin A in RPMI medium supplemented with 0.2% FBS, 2 mM GlutaMAX and 100 U mL À1 Pen/Strep.…”

Section: Cell Culturementioning

confidence: 99%

Dynamic self-organization of microwell-aggregated cellular mixtures

et al. 2016

Self Cite

View full text Add to dashboard Cite

Cells with different cohesive properties self-assemble in a spatiotemporal and context-dependent manner. Previous studies on cell self-organization mainly focused on the spontaneous structural development within a short period of time during which the cell numbers remained constant. However the effect of cell proliferation over time on the self-organization of cells is largely unexplored. Here, we studied the spatiotemporal dynamics of self-organization of a co-culture of MDA-MB-231 and MCF10A cells seeded in a well defined space (i.e. non-adherent microfabricated wells). When cell-growth was chemically inhibited, high cohesive MCF10A cells formed a core surrounded by low cohesive MDA-MB-231 cells on the periphery, consistent with the differential adhesion hypothesis (DAH). Interestingly, this aggregate morphology was completely inverted when the cells were free to grow. At an initial seeding ratio of 1 : 1 (MDA-MB-231 : MCF10A), the fast growing MCF10A cells segregated in the periphery while the slow growing MDA-MB-231 cells stayed in the core. Another morphology developed at an inequal seeding ratio (4 : 1), that is, the cell mixtures developed a side-by-side aggregate morphology. We conclude that the cell self-organization depends not only on the cell cohesive properties but also on the cell seeding ratio and proliferation. Furthermore, by taking advantage of the cell self-organization, we purified human embryonic stem cells-derived pancreatic progenitors (hESCs-PPs) from co-cultured feeder cells without using any additional tools or labels.

show abstract

Endothelial Cells Control Pancreatic Cell Fate at Defined Stages through EGFL7 Signaling

Cited by 40 publications

References 17 publications

Understanding the Pathophysiology and Challenges of Development of Medical Countermeasures for Radiation-Induced Vascular/Endothelial Cell Injuries: Report of a NIAID Workshop, August 20, 2015

Understanding the Pathophysiology and Challenges of Development of Medical Countermeasures for Radiation-Induced Vascular/Endothelial Cell Injuries: Report of a NIAID Workshop, August 20, 2015

Angiocrine functions of organ-specific endothelial cells

Dynamic self-organization of microwell-aggregated cellular mixtures

Contact Info

Product

Resources

About