Low‐intensity pulsed ultrasound‐induced ATP increases bone formation via the P2X7 receptor in osteoblast‐like MC3T3‐E1 cells

Manaka, Soichiro; Tanabe, Natsuko; Kariya, Taro; Naito, Masako; Takayama, Tadahiro; Nagao, Mike A.; Liu, Di; Ito, Koichi; Maeno, Masao; Suzuki, Naoto; Miyazaki, Masashi

doi:10.1016/j.febslet.2014.12.013

Cited by 49 publications

(47 citation statements)

References 39 publications

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“…P2X7 receptor also promotes osteogenesis by inducing the proliferation of osteoblast as well as osteodeposition through other pathways including c‐fos, ERK, and PI3K (Grol, Zelner, & Dixon, ; Liu et al, ; Okumura, Shiba, Kubo, & Yokoyama, ). Similarly, P2X7 receptor increases bone formation via promoting the differentiation of osteoblasts and osteogenesis by increasing intracellular Ca 2+ response in osteoblast‐like MC3T3‐E1 cells (Manaka et al, ). In osteoblast‐like MC3T3‐E1 cells, caveolin‐1 protein can attenuate P2X7 receptor‐mediated mineralization (Gangadharan, Nohe, Caplan, Czymmek, & Duncan, ).…”

Section: The Effect Of P2x7 Receptor On Bone Diseasesmentioning

confidence: 99%

P2X7, a critical regulator and potential target for bone and joint diseases

Zeng

Yao

Zhao

2018

Journal Cellular Physiology

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Abundant evidence indicted that P2X7 receptor show a essential role in human health and some human diseases including hypertension, atherosclerosis, pulmonary inflammation, tuberculosis infection, psychiatric disorders, and cancer. P2X7 receptor also has an important role in some central nervous system diseases such as neurodegenerative disorders. Recently, more research suggested that P2X7 receptor also plays a crucial role in bone and joint diseases. But the effect of P2X7 receptor on skeletal and joint diseases has not been systematically reviewed. In this article, the role of P2X7 receptor in skeletal and joint diseases is elaborated. The activation of P2X7 receptor can ameliorate osteoporosis by inducing a fine balance between osteoclastic resorption and osteoblastic bone formation. The activation of P2X7 receptor can relieve the stress fracture injury by increasing the response to mechanical loading and inducing osteogenesis. But the activation of P2X7 receptor mediates the cell growth and cell proliferation in bone cancer. In addition, the activation of P2X7 receptor can aggravate the process of some joint diseases such as osteoarthritis, rheumatoid arthritis, and acute gouty arthritis. The inhibition of P2X7 receptor can alleviate the pathological process of joint disease to some extent. In conclusion, P2X7 receptor may be a critical regulator and therapeutic target for bone and joint diseases.

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Section: The Effect Of P2x7 Receptor On Bone Diseasesmentioning

confidence: 99%

P2X7, a critical regulator and potential target for bone and joint diseases

Zeng

Yao

Zhao

2018

Journal Cellular Physiology

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“…However, most studies regarding the influence of LIPUS on bone metabolism have used osteoblast cell lines (Bandow et al, 2007;Katiyar et al, 2014;Manaka et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound

Suzuki

Hanmoto

Yano

et al. 2016

Comparative Biochemistry and Physiology Part A: Molecular &

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Using fish scales in which osteoclasts and osteoblasts coexist on the calcified bone matrix, we examined the effects of low-intensity pulsed ultrasound (LIPUS) on both osteoclasts and osteoblasts. At 3 hours of incubation after LIPUS treatment, osteoclastic markers such as tartrate-resistant acid phosphatase (TRAP) and cathepsin K mRNA expressions decreased significantly while mRNA expressions of osteoblastic markers, osteocalcin, distal-less homeobox 5, runt-related transcription factor 2a, and runt-related transcription factor 2b, increased significantly. At 6 and 18 hours of incubation, however, both osteoclastic and osteoblastic marker mRNA expression did not change at least present conditions. Using GeneChip analysis of zebrafish scales treated with LIPUS, we found that cell death-related genes were upregulated with LIPUS treatment. Real-time PCR analysis indicated that the expression of apoptosis-related genes also increased significantly. To confirm the involvement of apoptosis in osteoclasts with LIPUS, osteoclasts were induced by autotransplanting scales in goldfish. Thereafter, the DNA fragmentation associated with apoptosis was detected in the osteoclasts using the TUNEL (TdTmediated dUTP nick-end labeling) method. The multi-nuclei of TRAP-stained osteoclasts in the scales were labeled with TUNEL. TUNEL staining showed that the number of apoptotic osteoclasts in goldfish scales was significantly elevated by treatment with LIPUS at 3 hours of incubation. Thus, we are the first to demonstrate that LIPUS directly functions to osteoclasts and to conclude that LIPUS directly causes apoptosis in osteoclasts shortly after exposure.Key words: LIPUS, osteoclasts, osteoblasts, fish scales, apoptosis, GeneChip analysis Abbreviations: ALP, alkaline phosphatase; Bcl-2, B-cell lymphoma 2; CTSK, Cathepsin K; Dlx5, distal less homeobox5; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; LIPUS, low-intensity pulsed ultrasound; OCN, osteocalcin; PMAIP, phorbol-12-myristate-13-acetateinduced protein 1; SAFHS, sonic accelerated fracture healing system; pNP, para-nitrophenol; TRAP, tartrate-resistant acid phosphatase; TUNEL, TdT-mediated dUTP nick end labelling; TNFRSF10A, tumor necrosis factor receptor superfamily, member a; Runx2a, runt related transcription factor 2a; Runx2b, runt related transcription factor 2b 3

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“…Similarly, we also observed these findings in our study, suggesting that low‐intensity pulsed US could promote the traumatic vertebral fracture–healing process. Low‐intensity pulsed US has been found to produce a transdermal physical force that may stimulate osteoblast delivery to bone defects and directly affect osteogenic cells in vitro . Veronick et al found that as the low‐intensity pulsed US amplitude increased, the spatial intensity of bone increased as well, and there were more osteoblasts in the low‐intensity pulsed US‐treated area.…”

Section: Discussionmentioning

confidence: 99%

“…Lowintensity pulsed US has been found to produce a transdermal physical force that may stimulate osteoblast delivery to bone defects and directly affect osteogenic cells in vitro. 19,20 Veronick et al 21 found that as the lowintensity pulsed US amplitude increased, the spatial intensity of bone increased as well, and there were more osteoblasts in the low-intensity pulsed US-treated area. In our study, a larger quantity of osteoblasts was found in the traumatic vertebral fracture sites, and there were significantly more osteoblasts and newly formed bone marrow in the low-intensity pulsed US treatment group compared to the control group.…”

Section: Investigated the Effects Of Lowintensity Pulsed Us On The Hementioning

confidence: 99%

Low‐Intensity Pulsed Ultrasound Accelerates Traumatic Vertebral Fracture Healing by Coupling Proliferation of Type H Microvessels

Sun

et al. 2018

J of Ultrasound Medicine

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Low‐intensity pulsed ultrasound‐induced ATP increases bone formation via the P2X7 receptor in osteoblast‐like MC3T3‐E1 cells

Cited by 49 publications

References 39 publications

P2X7, a critical regulator and potential target for bone and joint diseases

P2X7, a critical regulator and potential target for bone and joint diseases

Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound

Low‐Intensity Pulsed Ultrasound Accelerates Traumatic Vertebral Fracture Healing by Coupling Proliferation of Type H Microvessels

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