Early Stages of Bone Fracture Healing: Formation of a Fibrin–Collagen Scaffold in the Fracture Hematoma

Echeverri, Fernando; Herrero, Miguel A.; López, José M.; Oleaga, Gerardo E.

doi:10.1007/s11538-014-0055-3

Cited by 43 publications

(31 citation statements)

References 59 publications

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“…A fibrin mesh is the main constituent of a fracture haematoma (Echeverri et al ., ). There is also a temporal component to the healing properties of the fracture haematoma; 4 day haematomas from rat femur fracture sites transplanted into intramuscular sites could generate fresh bone, whereas 2 day haematomas failed to do so (Mizuno et al ., ), which is a strong indication that haematomas must ‘mature’ before they are capable of contributing to the bone‐healing process.…”

Section: Discussionmentioning

confidence: 97%

Structural properties of fracture haematoma: current status and future clinical implications

Wang

Friis

Glatt

et al. 2016

J Tissue Eng Regen Med

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Blood clots (haematomas) that form immediately following a bone fracture have been shown to be vital for the subsequent healing process. During the clotting process, a number of factors can influence the fibrin clot structure, such as fibrin polymerization, growth factor binding, cellular infiltration (including platelet retraction), protein concentrations and cytokines. The modulation of the fibrin clot structure within the fracture site has important clinical implications and could result in the development of multifunctional scaffolds that mimic the natural structure of a haematoma. Artificial haematoma structures such as these can be created from the patient's own blood and can therefore act as an ideal bone defect filling material for potential clinical application to accelerate bone regeneration. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 97%

Structural properties of fracture haematoma: current status and future clinical implications

Wang

Friis

Glatt

et al. 2016

J Tissue Eng Regen Med

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“…Through the increased collagen fibrillar density, a native matrix scaffold that mimics the microstructure (and stiffness) of the osteoid is produced, which provides both biological and mechanical cues to accelerate osteogenic differentiation of three-dimensionally seeded cells. Furthermore, the clinical use of collagen scaffolds is successful and widely accepted with excellent biodegradation, and biocompatibility434445. In particular, previous studies have shown that collagen-based scaffolds are substitutable materials46, which in combination with MSCs can promote mineralization and heal a large bone unicortical defect16.…”

Section: Discussionmentioning

confidence: 99%

Accelerated craniofacial bone regeneration through dense collagen gel scaffolds seeded with dental pulp stem cells

Chamieh¹,

Collignon²,

Coyac³

et al. 2016

Sci Rep

136

112

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Therapies using mesenchymal stem cell (MSC) seeded scaffolds may be applicable to various fields of regenerative medicine, including craniomaxillofacial surgery. Plastic compression of collagen scaffolds seeded with MSC has been shown to enhance the osteogenic differentiation of MSC as it increases the collagen fibrillary density. The aim of the present study was to evaluate the osteogenic effects of dense collagen gel scaffolds seeded with mesenchymal dental pulp stem cells (DPSC) on bone regeneration in a rat critical-size calvarial defect model. Two symmetrical full-thickness defects were created (5 mm diameter) and filled with either a rat DPSC-containing dense collagen gel scaffold (n = 15), or an acellular scaffold (n = 15). Animals were imaged in vivo by microcomputer tomography (Micro-CT) once a week during 5 weeks, whereas some animals were sacrificed each week for histology and histomorphometry analysis. Bone mineral density and bone micro-architectural parameters were significantly increased when DPSC-seeded scaffolds were used. Histological and histomorphometrical data also revealed significant increases in fibrous connective and mineralized tissue volume when DPSC-seeded scaffolds were used, associated with expression of type I collagen, osteoblast-associated alkaline phosphatase and osteoclastic-related tartrate-resistant acid phosphatase. Results demonstrate the potential of DPSC-loaded-dense collagen gel scaffolds to benefit of bone healing process.

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“…The hematoma fills the fracture gap in the initial step of bone healing, acts as temporary scaffolding for cellular activity, and provides an appropriate environment for the subsequent biologic cascade of events ultimately resulting in healing (Chung et al, 2006, Axelrad and Einhorn, 2009, Echeverri et al, 2015). The hematoma first transitions to granulation tissue (Kolar et al, 2011).…”

Section: Hematoma Phasementioning

confidence: 99%

Bone fracture healing in mechanobiological modeling: A review of principles and methods

et al. 2017

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Bone fracture is a very common body injury. The healing process is physiologically complex, involving both biological and mechanical aspects. Following a fracture, cell migration, cell/tissue differentiation, tissue synthesis, and cytokine and growth factor release occur, regulated by the mechanical environment. Over the past decade, bone healing simulation and modeling has been employed to understand its details and mechanisms, to investigate specific clinical questions, and to design healing strategies. The goal of this effort is to review the history and the most recent work in bone healing simulations with an emphasis on both biological and mechanical properties. Therefore, we provide a brief review of the biology of bone fracture repair, followed by an outline of the key growth factors and mechanical factors influencing it. We then compare different methodologies of bone healing simulation, including conceptual modeling (qualitative modeling of bone healing to understand the general mechanisms), biological modeling (considering only the biological factors and processes), and mechanobiological modeling (considering both biological aspects and mechanical environment). Finally we evaluate different components and clinical applications of bone healing simulation such as mechanical stimuli, phases of bone healing, and angiogenesis.

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Early Stages of Bone Fracture Healing: Formation of a Fibrin–Collagen Scaffold in the Fracture Hematoma

Cited by 43 publications

References 59 publications

Structural properties of fracture haematoma: current status and future clinical implications

Structural properties of fracture haematoma: current status and future clinical implications

Accelerated craniofacial bone regeneration through dense collagen gel scaffolds seeded with dental pulp stem cells

Bone fracture healing in mechanobiological modeling: A review of principles and methods

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