Multidrug resistance gene and its relationship to ulcerative colitis and immune status of ulcerative colitis

Abstract

ABSTRACT. We examined the relationship among the multidrug resistance (MDR1) gene product P-glycoprotein (P-gp), ulcerative colitis, and immune status under ulcerative colitis. MDR1 P-gp expression and interleukin-8 levels in ulcerative colitis were determined using immunohistochemistry and a double-antibody sandwich avidinbiotin complex-enzyme-linked immunosorbent assay, respectively. Nitric oxide content and nitric oxide synthase activity in the colonic mucosa were determined using a colorimetric method; CD4 + and CD25 + T cell subset percentages in the peripheral blood were determined by flow cytometry. The positive expression rate of P-gp in patients with ulcerative colitis (17.4%) was significantly lower than that in the control group (31.4%). The expression rate decreased to 10.1, 9.2, and 8.3% after 12, 18, and 24 months of treatment, respectively, which were significantly lower than the expression rate before treatment (17.4%). P-gp expression levels during the remission phase and active phase of ulcerative colitis were 15.2 and 17.1%, respectively, which were significantly lower than that in normal controls (31.4%). Compared with P-gp-negative patients, nitric oxide content, nitric oxide synthase activity, and interleukin-8 levels were significantly higher in P-gppositive patients with moderately active, severely active, early onset, chronic relapsing, chronic persistent, and acute fulminant ulcerative colitis. CD4 + and CD25 + T cell subsets were significantly lower in the peripheral blood of patients with severely active and acute fulminant ulcerative colitis than in control subjects. Expression of the multidrug resistance gene and its product P-gp was observed in normal colon tissues and may be closely related to ulcerative colitis.

Identification of 6 cuproptosis-related genes for active ulcerative colitis with both diagnostic and therapeutic values

“…There have been significant advances in immunotherapy for UC over the past few decades, although the causes of the absence of immune responses in some patients are not known. [27,28] Recently, copper-dependent cell death, also known as cuproptosis, has been linked to disease progression. [29][30][31] However, there have been few studies on the specific mechanisms of cuproptosis in relation to active UC and its regulatory role has not yet been clarified.…”

Protective effect of five-flavor sophora flavescens enteric-coated capsules on inflammatory bowel disease and its molecular mechanism

“…Modern medicine generally believes that the factors of this disease mainly include immune disorders, infection, environment, genetics, psychology and so on (Lu et al, 2022;Zhu et al, 2022). Although the therapeutic strategies of western medicine often choose salicylic acid preparations, glucocorticoids, immunosuppressive agents and other means, there are still recurrent attacks after drug withdrawal, accompanied by many adverse reactions, and even appeared some problems (consisting of drug resistance, drug dependence and so on) (Ceng et al, 2021;Zhang et al, 2014). So far, the clinical treatment of UC remains difficult, and there is still a lack of specific and targeted therapeutic drugs.…”

Modulation of P-glycoprotein Expression and Function under Disease States in Rats and Humans

“…Provenzani et al reported that kidney transplant recipients carrying the ABCB1 (P-gp) 2677T/A allele required significantly higher daily tacrolimus doses than subjects homozygous for the wild-type allele [23]. Zhang et al reported that the expression rate of P-gp in patients with ulcerative colitis was significantly lower than that in the control group [24]. Andersen et al reported that the low P-gp protein levels, high MRP2 gene expression and low BCRP gene expression in intestinal tissue are early events in colorectal carcinogenesis through increasing intracellular exposure to carcinogenic substrates [25,26].…”