Heart Donation After Cardiac Death: Preliminary Study on an Isolated, Perfused Swine Heart After 20 Minutes of Normothermic Ischemia

Desrois, Martine; Piccardo, Alessandro; Zogheib, Élie; Dalmasso, Christiane; Lan, Carole; Fourré, D.; Cozzone, Patrick J.; Caus, Thierry; Bernard, Monique

doi:10.1016/j.transproceed.2014.04.021

Cited by 6 publications

(4 citation statements)

References 24 publications

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“…Cold ischemia is practically inevitable due to the need of transporting the donor organ [ 41 , 42 ]. Storage solutions have been optimized to preserve organs during transportation [ 50 ]. Heart preservation goes through two processes: the cessation of heartbeat, using a cold cardioplegic solution, and the cold storage [ 41 ].…”

Section: Myocardial Injury During Organ Procurementmentioning

confidence: 99%

“…The storage can then be carried out in two different ways, either by using static cold storage (SCS) or by hypothermic machine perfusion (HMP), which is capable of giving metabolic support [ 41 ]. In preclinical studies, the HMP is found superior to the SCS in preserving the DCD hearts because of the increased metabolic support [ 50 , 51 ].…”

Section: Myocardial Injury During Organ Procurementmentioning

confidence: 99%

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Targeting the Innate Immune Response to Improve Cardiac Graft Recovery after Heart Transplantation: Implications for the Donation after Cardiac Death

Toldo

Quader

Salloum

et al. 2016

IJMS

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Heart transplantation (HTx) is the ultimate treatment for end-stage heart failure. The number of patients on waiting lists for heart transplants, however, is much higher than the number of available organs. The shortage of donor hearts is a serious concern since the population affected by heart failure is constantly increasing. Furthermore, the long-term success of HTx poses some challenges despite the improvement in the management of the short-term complications and in the methods to limit graft rejection. Myocardial injury occurs during transplantation. Injury initiated in the donor as result of brain or cardiac death is exacerbated by organ procurement and storage, and is ultimately amplified by reperfusion injury at the time of transplantation. The innate immune system is a mechanism of first-line defense against pathogens and cell injury. Innate immunity is activated during myocardial injury and produces deleterious effects on the heart structure and function. Here, we briefly discuss the role of the innate immunity in the initiation of myocardial injury, with particular focus on the Toll-like receptors and inflammasome, and how to potentially expand the donor population by targeting the innate immune response.

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Section: Myocardial Injury During Organ Procurementmentioning

confidence: 99%

Section: Myocardial Injury During Organ Procurementmentioning

confidence: 99%

Targeting the Innate Immune Response to Improve Cardiac Graft Recovery after Heart Transplantation: Implications for the Donation after Cardiac Death

Toldo

Quader

Salloum

et al. 2016

IJMS

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“…The models remain an essential tool for predicting and preventing these diseases (Zaragoza et al, ). The pig is used to model cardiovascular events: atherosclerosis (Gerrity et al, ), myocardial infarction (Suzuki et al, ), abdominal aortic aneurysm (Molácek et al, ), heart failure (Dixon and Spinale, ), cardiac arrhythmias (Weiss et al, ; Zhang et al, ), heart transplantation (Desrois et al, ), etc. The pig remains a leading model for investigating human cardiovascular diseases due to anatomical and physiological similarities to the human heart.…”

mentioning

confidence: 99%

Neuroanatomy of the Pig Cardiac Ventricles. A Stereomicroscopic, Confocal and Electron Microscope Study

Paužienė

Rysevaitė-Kyguolienė

Alaburda

et al. 2017

The Anatomical Record

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Although the pig is a model for heart disease, the neuroanatomy of cardiac ventricles (CV) in this species remains undetailed. We aimed to define the innervation pattern of pig CV, combining histochemistry for acetylcholinesterase, immunofluorescent labeling and electron microscopy. Forty nine examined pig hearts show that the major nerves supplying the ventral side of CV descend from the venous part of the heart hilum. Fewer in number and smaller in size, epicardial nerves supply the dorsal half of the CV. Epicardial nerves on the left ventricle are thicker than those on the right. Ventricular ganglia of various sizes distribute at the basal level of both CV. Averagely, we found 3,848 ventricular neuronal somata per heart. The majority of somata were cholinergic, although ganglionic cells of different neurochemical phenotypes (positive for nNOS, ChAT/nNOS, or ChAT/TH) were also observed. Large and most numerous nerves proceeded within the epicardium. Most of endocardium and myocardium contained a network of nerve bundles and nerve fibers (NFs). But, a large number of thin nerves extended along the bundle of His and its branches. The majority of NFs were adrenergic, while cholinergic NFs were scarce yet more abundant than nitrergic ones. Sensory NFs positive for CGRP were the second most abundant phenotype after adrenergic NFs in all layers of the ventricular wall. Electron microscopy elucidated that ultrastructure of nerves varied between different areas of CV. The described structural organization of CV provides an anatomical basis for further functional and pathophysiological studies in the pig heart. Anat Rec, 2017. © 2017 Wiley Periodicals, Inc. Anat Rec, 300:1756-1780, 2017. © 2017 Wiley Periodicals, Inc.

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“…Scientists have focused on investigating the role of different physiological and biochemical conditions to preserve high energy phosphate stores ultimately reducing ischemic reperfusion injury. In a study on pig hearts, normothermic ischemia in hearts isolated for transplantation had lower ATP as compared to those exposed to immediate reperfusion [25]. However, studies on pigs have shown no effect of cold static storage on high energy phosphate content in hearts from brain-dead animals [26].…”

Section: Discussionmentioning

confidence: 95%

Fingolimod (FTY720) Preserves High Energy Phosphates and Improves Cardiac Function in Heterotopic Heart Transplantation Model

Ahmed

Farooq

Sadiq

et al. 2020

IJMS

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During heart transplantation, donor heart leads to reduced oxygen supply resulting in low level of high energy phosphate (HEP) reserves in cardiomyocyte. Lower HEP is one of the underlying reasons of cell death due to ischemia. In this study we investigated the role of Fingolimod (FTY720) in heart transplantation ischemia. Eight groups of Sprague-Dawley rats (n = 5 for each subgroup) were made, A1 and C1 were given FTY720 1 mg/kg while B1 and D1 were given normal saline. The hearts were implanted into another set of similar rats after preservation period of 1 h at 4–8 °C. Significantly higher Left ventricular systolic pressure (LVSP), dP/dT maximum (p < 0.05), dP/dT minimum (p < 0.05) were recorded in the FTY720 treated group after 24 h of reperfusion while after 1 h of reperfusion, there were no significant differences in LVSP, maximum and negative dP/dT, and Left ventricular end diastolic pressure (LVEDP) between the control and the FTY720-treated transplant groups. Coronary blood flow (CBF) was enhanced (p < 0.05) in the FTY720 treated group after 1 and 24 h. ATP p < 0.001, p < 0.05 at 1 and 24 h, ADP p < 0.001, p > 0.05 at 1 and 24 h, and phosphocreatine p < 0.05, p > 0.05 at 1 and 24 h were better preserved by FTY720 treatment as compared to control group. The study concluded that pretreatment of grafted hearts with FTY720 improved hemodynamics, CBF, high energy phosphate reserves, reduces the peroxynitrite level and poly (ADP ribose) polymerase (PARP) inhibition that prevents ischemia-reperfusion injury.

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Heart Donation After Cardiac Death: Preliminary Study on an Isolated, Perfused Swine Heart After 20 Minutes of Normothermic Ischemia

Cited by 6 publications

References 24 publications

Targeting the Innate Immune Response to Improve Cardiac Graft Recovery after Heart Transplantation: Implications for the Donation after Cardiac Death

Targeting the Innate Immune Response to Improve Cardiac Graft Recovery after Heart Transplantation: Implications for the Donation after Cardiac Death

Neuroanatomy of the Pig Cardiac Ventricles. A Stereomicroscopic, Confocal and Electron Microscope Study

Fingolimod (FTY720) Preserves High Energy Phosphates and Improves Cardiac Function in Heterotopic Heart Transplantation Model

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