Topical Hesperidin Enhances Epidermal Function in an Aged Murine Model

Man, George; Mauro, Theodora M.; Zhai, Yongjiao; Kim, Peggy L.; Cheung, Carolyn; Hupe, Melanie; Crumrine, Debbie; Elias, Peter M.; Man, Mao‐Qiang

doi:10.1038/jid.2014.486

Cited by 26 publications

(35 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In support of our hypothesis, both psoriasis and atopic dermatitis display prominent abnormalities in epidermal function, which has been proposed to ‘drive’ the inflammation of these disorders (Elias and Steinhoff, 2008; Man et al, 2015, Sano, 2015), and elevated serum cytokine levels correlate with severity of these disorders (Arican et al, 2005; Jacob et al, 2003; Yamamoto et al, 2013; Yoshizawa et al, 2002). However, the origin of serum cytokines in these two inflammatory dermatoses is controversial since these disorders are considered to be T cell-mediated, immune diseases.…”

Section: Introductionsupporting

confidence: 83%

“…Therefore, we next determined whether correction of the epidermal functional abnormalities with topical petrolatum lowers cutaneous and serum cytokine levels in aged mice. We initially treated 12-month old C57BL/6J mice, which exhibit epidermal permeability barrier dysfunction (Ghadially et al, 1995; Man et al, 2015), with topical petrolatum, an agent previously shown to improve epidermal permeability barrier function in aged murine skin (Ghadially et al, 1992; Mao-Qiang et al, 1995), three times daily for 10 days. As seen in Figure 4a, topical petrolatum treatment lowered mRNA levels of cutaneous cytokines to levels comparable to those that occur in the skin of young mice (dotted line, setting levels in young mice as 100%).…”

Section: Resultsmentioning

confidence: 99%

“…Relative expression of the mRNAs compared to mRNA in normal young mice was calculated. Data are expressed as percentage of control (setting normal young controls as 100%) (Man et al, 2015). …”

Section: Methodsmentioning

confidence: 99%

“…Because previous studies from our group and others demonstrated that chronologically aged skin displays both compromised permeability homeostasis and reduced stratum corneum hydration, both of which increase cutaneous cytokine production (Choi et al, 2007; Ghadially et al, 1995; Kikuchi et al, 2003; Man et al, 2015; Tsai et al, 1994; Wood et al, 1992 & 1997), and increased susceptibility to infections (Laube, 2004), we hypothesized that epidermal dysfunction-induced cutaneous inflammation could lead to elevations in serum cytokines. In support of our hypothesis, both psoriasis and atopic dermatitis display prominent abnormalities in epidermal function, which has been proposed to ‘drive’ the inflammation of these disorders (Elias and Steinhoff, 2008; Man et al, 2015, Sano, 2015), and elevated serum cytokine levels correlate with severity of these disorders (Arican et al, 2005; Jacob et al, 2003; Yamamoto et al, 2013; Yoshizawa et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Epidermal Dysfunction Leads to an Age-Associated Increase in Levels of Serum Inflammatory Cytokines

Mauro

Dang

et al. 2017

Journal of Investigative Dermatology

Self Cite

100

117

View full text Add to dashboard Cite

Even though elderly populations lack visible or other clinical signs of inflammation, their serum cytokine and C reactive protein levels typically are elevated. However, the origin of age-associated systemic inflammation is unknown. Our previous studies showed that abnormalities in epidermal function provoke cutaneous inflammation, and because intrinsically aged skin displays compromised permeability barrier homeostasis, as well as reduced stratum corneum hydration, we hypothesized here that epidermal dysfunction could contribute to the elevations in serum cytokines in the elderly. Our results show first that acute disruption of the epidermal permeability barrier in young mice led not only to a rapid increase in cutaneous cytokine mRNA expression, but also an increase in serum cytokine levels. Second, cytokine levels in both the skin and serum increased in otherwise normal, aged mice (>12 months). Third, expression of TNFα and amyloid A mRNA levels increased in the epidermis, but not in the liver, in parallel with significant elevations in serum levels of cytokines. Fourth, disruption of the permeability barrier induced similar elevations in epidermal and serum cytokine levels in normal and athymic mice, suggesting the T cells play a negligible role in the elevations in cutaneous and serum inflammatory cytokines induced by epidermal dysfunction. Fifth, correction of epidermal function significantly reduced cytokine levels not only in the skin, but also in the serum of aged mice. Together, these results indicate that the sustained abnormalities in epidermal function in chronologically aged skin contribute to the elevated serum levels of inflammatory cytokines, potentially predisposing the elderly to the subsequent development or exacerbation of chronic inflammatory disorders.

show abstract

Section: Introductionsupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Epidermal Dysfunction Leads to an Age-Associated Increase in Levels of Serum Inflammatory Cytokines

Mauro

Dang

et al. 2017

Journal of Investigative Dermatology

Self Cite

100

117

View full text Add to dashboard Cite

show abstract

“…Following twice‐daily treatments with Hirudoid ® cream for 7 days, epidermal biophysical properties were measured using respective probe connected to an MPA5 physiology monitor (Courage+Khazaka Electronic GmbH, Cologne, Germany) . Permeability barrier recovery rates were assessed 2 and 4 hours after acute barrier disruption with tape stripping …”

Section: Experiments Designmentioning

confidence: 99%

A topical heparinoid‐containing product improves epidermal permeability barrier homeostasis in mice

Yao

Guo

Feng

et al. 2019

Experimental Dermatology

Self Cite

View full text Add to dashboard Cite

Because of the importance of epidermal functions, including stratum corneum hydration and maintenance of permeability barrier homeostasis, in the pathogenesis of a variety of cutaneous and systemic disorders, a wide range of products has been developed to improve epidermal functions. However, the underlying mechanisms whereby certain products, including heparinoid‐containing product, are far little understood. In the present study, we assessed the impact of a heparinoid‐containing product, Hirudoid® cream, on epidermal permeability barrier function and expression levels of a panel of epidermal mRNA related to the formation/maintenance of the permeability barrier in mouse skin. Our results showed that while the baseline levels of transepidermal water rates remained unchanged, treatment with Hirudoid® cream twice daily for 7 days significantly accelerated permeability barrier recovery and increased stratum corneum hydration. In parallel, expression levels of epidermal mRNA for certain differentiation marker‐related proteins, lipid synthetic enzymes, keratinocyte proliferation and antimicrobial peptides also increased significantly. Together, these results provide the underlying mechanisms by which topical Hirudoid® cream improves epidermal permeability barrier and antimicrobial function. Because of its benefits for epidermal functions, heparinoid‐containing product could be more useful in the management of skin conditions, characterized by abnormal permeability barrier and antimicrobial function.

show abstract

Effect of Chimpi, dried citrus peel, on aquaporin-3 expression in HaCaT human epidermal keratinocytes

et al. 2022

View full text Add to dashboard Cite

Topical Hesperidin Enhances Epidermal Function in an Aged Murine Model

Cited by 26 publications

References 15 publications

Epidermal Dysfunction Leads to an Age-Associated Increase in Levels of Serum Inflammatory Cytokines

Epidermal Dysfunction Leads to an Age-Associated Increase in Levels of Serum Inflammatory Cytokines

A topical heparinoid‐containing product improves epidermal permeability barrier homeostasis in mice

Effect of Chimpi, dried citrus peel, on aquaporin-3 expression in HaCaT human epidermal keratinocytes

Contact Info

Product

Resources

About