Effects of Long-Term Feeding of the Polyphenols Resveratrol and Kaempferol in Obese Mice

Abstract

The effect of the intake of antioxidant polyphenols such as resveratrol and others on survival and different parameters of life quality has been a matter of debate in the last years. We have studied here the effects of the polyphenols resveratrol and kaempferol added to the diet in a murine model undergoing long-term hypercaloric diet. Using 50 mice for each condition, we have monitored weight, survival, biochemical parameters such as blood glucose, insulin, cholesterol, triglycerides and aspartate aminotransferase, neuromuscular coordination measured with the rotarod test and morphological aspect of stained sections of liver and heart histological samples. Our data show that mice fed since they are 3-months-old with hypercaloric diet supplemented with any of these polyphenols reduced their weight by about 5–7% with respect to the controls fed only with hypercaloric diet. We also observed that mice fed with any of the polyphenols had reduced levels of glucose, insulin and cholesterol, and better marks in the rotarod test, but only after 1 year of treatment, that is, during senescence. No effect was observed in the rest of the parameters studied. Furthermore, although treatment with hypercaloric diets induced large changes in the pattern of gene expression in liver, we found no significant changes in gene expression induced by the presence of any of the polyphenols. Thus, our data indicate that addition of resveratrol or kaempferol to mice food produces an initial decrease in weight in mice subjected to hypercaloric diet, but beneficial effects in other parameters such as blood glucose, insulin and cholesterol, and neuromuscular coordination, only appear after prolonged treatments.

Cocoa polyphenols and fiber modify colonic gene expression in rats

“…The administration of quercetin in mice fed with a high-fat diet reduces the expression of hepatic genes related to lipid and fatty acid production, such as Aldh1b1 , Abcg5 , Acaca , Fasn , Gpam and Apoa4 [ 38 ]. Although some other studies report body weight loss and a reduced fat deposition after polyphenol intake [ 38 , 43 , 44 ], previous studies carried out in our laboratory using three distinct cocoa materials with different proportions of polyphenols suggest that those effects on body weight cannot be exclusively attributed to cocoa polyphenols but also to other compounds present in cocoa [ 29 ]. As in the present study, we evidenced the lack of effect of the CF diet on body weight and on the same genes related to lipid metabolism as the entire cocoa diet, other bioactive compounds of cocoa, such as methylxanthines, could be proposed as being responsible for these effects on lipid metabolism gene expression proteins and body weight.…”

Suppression of Arrhythmia by Enhancing Mitochondrial Ca2+ Uptake in Catecholaminergic Ventricular Tachycardia Models

“…It is important to note that we did not observe any adverse effects of MiCUps in mice treated with efsevin or kaempferol for 3 to 8 consecutive days. Furthermore, kaempferol was previously used in animal experiments, and no adverse effects, even after up to 1 year of treatment or at high doses, were observed 42 , 43 , 44 . However, further long-term experiments and large animal studies are needed to further evaluate safety of a MiCUp-based therapy.…”

Polyphenols and their benefits: A review

“…[225,226] P38 is an MAPK that can regulate cell proliferation while EGFR can mediate p38 activation. [227,228] Studies have also examined the potential link between the consumption of foods containing kaempferol and the reduction of the risk of developing disorders such as cardiovascular diseases [229] , obesity [230,231] , inflammation [232], and type 2 diabetes. [230,233] Kaempferol and its glycosides were reported to reduce the accumulation of adipose tissue and improve hyperlipidemia and diabetes in obese mice by increasing lipid metabolism through the down-regulation of PPARγ and SREBP-1c.…”