Synthesis and Εvaluation of Αnticancer Αctivity in Cells of Novel Stoichiometric Pegylated Fullerene-Doxorubicin Conjugates

Magoulas, George E.; Bantzi, Marina; Messari, Danai; Voulgari, Efstathia; Gialeli, Chrysostomi; Barbouri, Despoina; Giannis, Athanassios; Karamanos, Nikos K.; Papaioannou, Dionissios; Avgoustakis, Konstantinos

doi:10.1007/s11095-014-1566-1

Cited by 23 publications

(18 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A coupling of Dox and C 60 for a passive targeting of cancer cells can be achieved by covalent linkage [15–17, 23] or by noncovalent interactions [18–22]. A complex of C 60 with two amide-linked Dox molecules showed the same cytotoxicity against human breast cancer MCF-7 cells as the free drug [16].…”

Section: Introductionmentioning

confidence: 99%

Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro

et al. 2019

View full text Add to dashboard Cite

Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C 60 fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells. Here, we studied the physicochemical properties and anticancer activity of C 60 fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C 60 -Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug’s level evidenced C 60 fullerene considerable nanocarrier function. The results of this study indicated that C 60 fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Fullerene–biomolecule conjugates exhibit a very high antineoplastic efficiency. For example, the chemical attachment of anticancer preparations such as Paclitaxel and Doxorubicin (Dox) to C 60 fullerene results in an improvement of these drugs’ pharmacokinetics and increases their therapeutic efficacy (Liu et al 2011 ; Magoulas et al 2015 ; Prylutska et al 2015 ). Previous investigations from our research group revealed that pristine C 60 fullerene possesses a potent anticancer activity per se (Prylutska et al 2011a , b ; Lynchak et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

C60 fullerene and its nanocomplexes with anticancer drugs modulate circulating phagocyte functions and dramatically increase ROS generation in transformed monocytes

et al. 2018

View full text Add to dashboard Cite

BackgroundC60 fullerene-based nanoformulations are proposed to have a direct toxic effect on tumor cells. Previous investigations demonstrated that C60 fullerene used alone or being conjugated with chemotherapeutic agents possesses a potent anticancer activity. The main aim of this study was to investigate the effect of C60 fullerene and its nanocomplexes with anticancer drugs on human phagocyte metabolic profile in vitro.MethodsAnalysis of the metabolic profile of phagocytes exposed to C60 fullerene in vitro revealed augmented phagocytic activity and down-regulated reactive nitrogen species generation in these cells. Additionally, cytofluorimetric analysis showed that C60 fullerene can exert direct cytotoxic effect on normal and transformed phagocytes through the vigorous induction of intracellular reactive oxygen species generation.ResultsCytotoxic action as well as the pro-oxidant effect of C60 fullerene was more pronounced toward malignant phagocytes. At the same time, C60 fullerenes have the ability to down-regulate the pro-oxidant effect of cisplatin on normal cells. These results indicate that C60 fullerenes may influence phagocyte metabolism and have both pro-oxidant and antioxidant properties.ConclusionsThe antineoplastic effect of C60 fullerene has been observed by direct toxic effect on tumor cells, as well as through the modulation of the functions of effector cells of antitumor immunity.

show abstract

“…116 Magoulas et al . 117 studied the properties of fullerene C 60 conjugated with one ( 48 ) and two ( 49 ) units of doxorubicin (DOX), a potent anticancer agent (Fig. 11).…”

Section: Anti Cancer Propertiesmentioning

confidence: 99%

Fullerenes in biology and medicine

et al. 2017

View full text Add to dashboard Cite

show abstract

Synthesis and Εvaluation of Αnticancer Αctivity in Cells of Novel Stoichiometric Pegylated Fullerene-Doxorubicin Conjugates

Cited by 23 publications

References 25 publications

Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro

Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro

C60 fullerene and its nanocomplexes with anticancer drugs modulate circulating phagocyte functions and dramatically increase ROS generation in transformed monocytes

Fullerenes in biology and medicine

Contact Info

Product

Resources

About