Complete Genome Assembly of Staphylococcus epidermidis AmMS 205

Davenport, Karen W.; Daligault, Hajnalka E.; Minogue, Timothy D.; Bishop‐Lilly, Kimberly A.; Broomall, S. M.; Bruce, David; Chain, Patrick; Coyne, S. R.; Frey, K. G.; Gibbons, Henry S.; Jaissle, J.; Redden, C. L.; Rosenzweig, C. Nicole; Scholz, Matthew; Teshima, Hazuki; Johnson, Shannon L.

doi:10.1128/genomea.01059-14

Cited by 5 publications

(5 citation statements)

References 10 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MscL also triggers the export of EfTu 21 , the Substance P sensor protein in S. epidermidis 6 . MscL is a ubiquitous protein and is also expressed by S. epidermidis 22 . As shown herein, CGRP increases the export of DnaK by S. epidermidis (Spot 7 in Fig.…”

Section: Resultsmentioning

confidence: 99%

Skin-bacteria communication: Involvement of the neurohormone Calcitonin Gene Related Peptide (CGRP) in the regulation of Staphylococcus epidermidis virulence

Ndiaye

Leclerc

Kentache

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Staphylococci can sense Substance P (SP) in skin, but this molecule is generally released by nerve terminals along with another neuropeptide, Calcitonin Gene Related Peptide (CGRP). In this study, we investigated the effects of αCGRP on Staphylococci. CGRP induced a strong stimulation of Staphylococcus epidermidis virulence with a low threshold (<10−12 M) whereas Staphylococcus aureus was insensitive to CGRP. We observed that CGRP-treated S. epidermidis induced interleukin 8 release by keratinocytes. This effect was associated with an increase in cathelicidin LL37 secretion. S. epidermidis displayed no change in virulence factors secretion but showed marked differences in surface properties. After exposure to CGRP, the adherence of S. epidermidis to keratinocytes increased, whereas its internalization and biofilm formation activity were reduced. These effects were correlated with an increase in surface hydrophobicity. The DnaK chaperone was identified as the S. epidermidis CGRP-binding protein. We further showed that the effects of CGRP were blocked by gadolinium chloride (GdCl3), an inhibitor of MscL mechanosensitive channels. In addition, GdCl3 inhibited the membrane translocation of EfTu, the Substance P sensor. This work reveals that through interaction with specific sensors S. epidermidis integrates different skin signals and consequently adapts its virulence.

show abstract

Section: Resultsmentioning

confidence: 99%

Skin-bacteria communication: Involvement of the neurohormone Calcitonin Gene Related Peptide (CGRP) in the regulation of Staphylococcus epidermidis virulence

Ndiaye

Leclerc

Kentache

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…For comparative genomics analysis genomes of six S. epidermidis reference strains were included: RP62A ( Gill et al 2005 ), ASM1192v1), ATCC12228 ( Zhang et al 2003 ), ASM764v1), SEI ( Davenport et al 2014 ), CP009046), 949_S8 ( Biswas et al 2015 ), CP010942), PM221 ( Savijoki et al 2014 ), HG813242), and BPH 0662 ( Jyh et al 2016 ), NZ_LT571449) together with 129 S. epidermidis genomes derived from two previously published collections ( Roach et al 2015 ; Tewhey et al 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Whole Genome Sequence and Comparative Genomics Analysis of Multi-drug Resistant Environmental Staphylococcus epidermidis ST59

Misra

Jamrozy

et al. 2018

G3 Genes|Genomes|Genetics

View full text Add to dashboard Cite

Staphylococcus epidermidis is a major opportunistic pathogen primarily recovered from device-associated healthcare associated infections (DA-HAIs). Although S. epidermidis and other coagulase-negative staphylococci (CoNS) are less virulent than Staphylococcus aureus, these bacteria are an important reservoir of antimicrobial resistance genes and resistance-associated mobile genetic elements that can be transferred between staphylococcal species. We report a whole genome sequence of a multidrug resistant S. epidermidis (strain G6_2) representing multilocus sequence type (ST) 59 and isolated from an environmental sampling of a hotel room in London, UK. The genome of S. epidermidis G6_2 comprises of a 2408357 bp chromosome and six plasmids, with an average G+C content of 32%. The strain displayed a multi-drug resistance phenotype which was associated with carriage of 7 antibiotic resistance genes (blaZ, mecA, msrA, mphC, fosB, aacA-aphD, tetK) as well as resistance-conferring mutations in fusA and ileS. Antibiotic resistance genes were located on plasmids and chromosome. Comparative genomic analysis revealed that antibiotic resistance gene composition found in G6_2 was partly preserved across the ST59 lineage.

show abstract

“…This gene encodes a protein with 82 amino acids, a membrane protein that possesses α-hemolysin activity [ 15 , 16 ]. Sixty-eight of these amino acids are identical to the hemolytic domain of a protein found in a S. epidermidis strain (NCBI: AIR83523.1), called “putative membrane protein insertion efficiency factor” [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus epidermidis and Staphylococcus haemolyticus: Molecular Detection of Cytotoxin and Enterotoxin Genes

Pinheiro

Brito

Oliveira

et al. 2015

Toxins

View full text Add to dashboard Cite

Although opportunistic pathogens, coagulase-negative staphylococci (CoNS), including Staphylococcus epidermidis and Staphylococcus haemolyticus, have long been regarded as avirulent organisms. The role of toxins in the development of infections caused by CoNS is still controversial. The objective of this study was to characterize the presence of enterotoxin and cytotoxin genes in S. epidermidis and S. haemolyticus isolates obtained from blood cultures. Cytotoxin genes were detected by PCR using novel species-specific primers. Among the 85 S. epidermidis and 84 S. haemolyticus isolates, 95.3% and 79.8%, respectively, carried at least one enterotoxin gene. The most frequent enterotoxin genes were sea (53.3%), seg (64.5%) and sei (67.5%). The seg gene was positively associated with S. epidermidis (p = 0.02), and this species was more toxigenic than S. haemolyticus. The hla/yidD gene was detected in 92.9% of S. epidermidis and the hla gene in 91.7% of S. haemolyticus isolates; hlb was detected in 92.9% of the S. epidermidis isolates and hld in 95.3%. Nosocomial Staphylococcus epidermidis and S. haemolyticus isolates exhibited a high toxigenic potential, mainly containing the non-classical enterotoxin genes seg and sei. The previously unreported detection of hla/yidD and hlb in S. epidermidis and S. haemolyticus using species-specific primers showed that these hemolysin genes differ between CoNS species and that they are highly frequent in blood culture isolates.

show abstract

Complete Genome Assembly of Staphylococcus epidermidis AmMS 205

Cited by 5 publications

References 10 publications

Skin-bacteria communication: Involvement of the neurohormone Calcitonin Gene Related Peptide (CGRP) in the regulation of Staphylococcus epidermidis virulence

Skin-bacteria communication: Involvement of the neurohormone Calcitonin Gene Related Peptide (CGRP) in the regulation of Staphylococcus epidermidis virulence

Whole Genome Sequence and Comparative Genomics Analysis of Multi-drug Resistant Environmental Staphylococcus epidermidis ST59

Staphylococcus epidermidis and Staphylococcus haemolyticus: Molecular Detection of Cytotoxin and Enterotoxin Genes

Contact Info

Product

Resources

About