Ano1 is a better marker than c-Kit for transcript analysis of single interstitial cells of Cajal in culture

Loera-Valencia, Raúl; Wang, Xuan-Yu; Wright, Gavin; Barajas‐López, Carlos; Huizinga, Jan D.

doi:10.2478/s11658-014-0214-4

Cited by 15 publications

(14 citation statements)

References 51 publications

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“…Another marker, considered by some authors even better than the c-Kit for the ICC identification (29), was recently found; the anoctamin 1 (ANO1). It is a Ca 2+ -activated chloride channel necessary for slow wave generation and devoid of any effect on ICC differentiation (30).…”

Section: Ano-1 Receptormentioning

confidence: 99%

Interstitial cells of Cajal and telocytes in the gut: twins, related or simply neighbor cells?

Vannucchi

Traini

2016

Biomolecular Concepts

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Abstract:In the interstitium of the connective tissue several types of cells occur. The fibroblasts, responsible for matrix formation, the mast cells, involved in local response to inflammatory stimuli, resident macrophages, plasma cells, lymphocytes, granulocytes and monocytes, all engaged in immunity responses. Recently, another type of interstitial cell, found in all organs so far examined, has been added to the previous ones, the telocytes (TC). In the gut, in addition to the cells listed above, there are also the interstitial cells of Cajal (ICC), a peculiar type of cell exclusively detected in the alimentary tract with multiple functions including pace-maker activity. The possibility that TC and ICC could correspond to a unique cell type, where the former would represent an ICC variant outside the gut, was initially considered, however, further studies have clearly shown that ICC and TC are two distinct types of cells. In the gut, while the features and the roles of the ICC are established, part of the scientific community is still disputing these 'new' interstitial cells to which several names such as fibroblast-like cells (FLCs), interstitial Cajal-like cells or, most recently, PDGFRα + cells have been attributed. This review will detail the main features and roles of the TC and ICC with the aim to establish their relationships and hopefully define the identity of the TC in the gut.

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Section: Ano-1 Receptormentioning

confidence: 99%

Interstitial cells of Cajal and telocytes in the gut: twins, related or simply neighbor cells?

Vannucchi

Traini

2016

Biomolecular Concepts

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“…C-kit labeling indirectly reflects the quantity and density of ICC [47,48]. It can not only maintain the phenotype of ICC, but also determine the differentiation, development, and rhythmic activity of ICC [49,50]. Our results indicate that YTC increased the expression level of C-kit and SCF, which protect the expression of ICC.…”

Section: Discussionmentioning

confidence: 67%

Laxative Effects of Yangyin Tongmi Capsule on a Model of Diphenoxylate‐Induced Constipation in Mice

Liu

Sui

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Constipation is characterized by reduced number of bowel movements, dry stools, and difficult defecation. Yangyin Tongmi capsule (YTC), a traditional Chinese formula, is used in the treatment of constipation, while the underlying mechanisms remain unknown. Herein, this work attempted to prove the effects of YTC on constipation treatment and its possible mechanisms. KM mice were randomly divided into four groups (n = 10/group) and treated with double distilled water (Control), diphenoxylate (Model: 10 mg/kg), or diphenoxylate plus low-dose YTC (L-YTC: 0.6 g/kg) or high-dose YTC (H-YTC: 1.2 g/kg). The data indicated that YTC can significantly shorten the discharge time of the first black stool, improve intestinal propulsion rate, and increase the water content and quantity of feces in mice. ELISA suggested that YTC regulate the content of intestinal hormones and neurotransmitters, such as motilin (MTL), gastrin (GT), somatostatin (SST), substance P (SP), acetylcholine (Ach), and nitric oxide (NO). The expression levels of aquaporin 3 (AQP3) and aquaporin 8 (AQP8) in the colon were examined by immunohistochemistry. In the meantime, the expression levels of P2X2, C-kit, and stem cell factor (SCF) in the colon were examined by western blot analysis. The results of this study suggest that YTC has mitigative effects on diphenoxylate-induced constipation by regulating the content of intestinal hormones and neurotransmitters and regulating the expression of related proteins in the colon.

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“…Furthermore, ICCs were identified by immunofluorescent double staining. ANO1 is a new and highly selective molecular marker for ICCs [ 34 ]. The cultured cells were positive for both c-kit and ANO1, indicating that they were ICCs.…”

Section: Discussionmentioning

confidence: 99%

Yangyin Runchang Decoction Improves Intestinal Motility in Mice with Atropine/Diphenoxylate‐Induced Slow‐Transit Constipation

Jiang

Zhou

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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This study assessed the efficacy and mechanism of action of Yangyin Runchang decoction (YRD) in the treatment of slow-transit constipation (STC). ICR mice were randomly divided into four groups (n = 10/group) and treated with saline (normal control; NC), atropine/diphenoxylate (model control; MC; 20 mg/kg), or atropine/diphenoxylate plus low-dose YRD (L-YRD; 29.6 g/kg) or high-dose YRD (H-YRD; 59.2 g/kg). Intestinal motility was assessed by evaluating feces and the intestinal transit rate (ITR). The serum level of stem cell factor (SCF) and changes in interstitial cells of Cajal (ICCs) were also evaluated. Additionally, the expression of SCF and c-kit and the intracellular Ca2+ concentration [Ca2+]I were investigated. Fecal volume and ITR were greater in the L-YRD and H-YRD groups than in the MC group. The serum SCF level was lower in the MC group than in the NC group; this effect was ameliorated in the YRD-treated mice. Additionally, YRD-treated mice had more ICCs and elevated expression of c-kit and membrane-bound SCF, and YRD also increased [Ca2+]I in vitro in isolated ICCs. YRD treatment in this STC mouse model was effective, possibly via the restoration of the SCF/c-kit pathway, increase in the ICC count, and enhancement of ICC function by increasing [Ca2+]i.

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Ano1 is a better marker than c-Kit for transcript analysis of single interstitial cells of Cajal in culture

Cited by 15 publications

References 51 publications

Interstitial cells of Cajal and telocytes in the gut: twins, related or simply neighbor cells?

Interstitial cells of Cajal and telocytes in the gut: twins, related or simply neighbor cells?

Laxative Effects of Yangyin Tongmi Capsule on a Model of Diphenoxylate‐Induced Constipation in Mice

Yangyin Runchang Decoction Improves Intestinal Motility in Mice with Atropine/Diphenoxylate‐Induced Slow‐Transit Constipation

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