Identification of microRNAs in Human Plasma

Eerden, Bram C. J. van der; Alves, Rodrigo D. A. M.; Kockx, Christel; Özgür, Zeliha; Schreuders‐Koedam, Marijke; Peppel, Jeroen van de; IJcken, Wilfred F. J. van; Leeuwen, Johannes P.T.M. van

doi:10.1007/978-1-4939-1619-1_7

Cited by 1 publication

(1 citation statement)

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“…Overall, to date more than 35,000 mature miRNAs from 223 species have been annotated in the miRBase database (release 21) [ 18 ]. Growing interest in miRNAs and technical capabilities have enabled researchers to identify these intriguing molecules not only in various tissues, cells and organs but also in different body fluids, e.g., serum [ 19 , 20 , 21 ], plasma [ 19 , 22 , 23 ], urine [ 24 , 25 , 26 ], breast milk [ 27 , 28 , 29 ], saliva [ 30 , 31 ], tears [ 32 ], semen [ 33 , 34 ], and others [ 32 , 35 ]. Contrary to traditional beliefs on extracellular RNA stability, circulating miRNAs are shown to be resistant to RNase activity and highly stable in extreme pH and temperature conditions [ 21 , 36 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

Plant MicroRNAs—Novel Players in Natural Medicine?

Łukasik

Zielenkiewicz

2016

IJMS

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MicroRNAs (miRNAs) represent a class of small non-coding RNAs that act as efficient gene expression regulators and thus play many important roles in living organisms. Due to their involvement in several known human pathological and pathogenic states, miRNA molecules have become an important issue in medicine and gained the attention of scientists from the pharmaceutical industry. In recent few years, a growing number of studies have provided evidence that miRNAs may be transferred from one species to another and regulate gene expression in the recipients’ cells. The most intriguing results revealed that stable miRNAs derived from food plants may enter the mammals’ circulatory system and, after reaching the target, inhibit the production of specific mammalian protein. Part of the scientific community has perceived this as an attractive hypothesis that may provide a foundation for novel therapeutic approaches. In turn, others are convinced about the “false positive” effect of performed experiments from which the mentioned results were achieved. In this article, we review the recent literature that provides evidence (from both fronts) of dietary, plant miRNA uptake and functionality in various consumers. Additionally, we discuss possible miRNA transport mechanisms from plant food sources to human cells.

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