A host beetle pheromone regulates development and behavior in the nematode Pristionchus pacificus

Cinkornpumin, Jessica; Wisidagama, Dona R.; Rapoport, Veronika; Go, James; Dieterich, Christoph; Wang, Xiaoyue; Sommer, Ralf J.; Hong, Ray L.

doi:10.7554/elife.03229

Cited by 32 publications

(43 citation statements)

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“…Nematode Strains 622 P. pacificus and C. elegans strains were maintained at 20°C on NGM plates 623 seeded with OP50 as described previously [68]. The following P. pacificus strains 624 were used: PS312 (California wild-type, synonymous with RS2333), Ppa-obi-625 1(tu404) ChrI, Ppa-dfc-1(tu391) ChrI, and csu60 ChrII; Ppa-daf-12(tu389) ChrX.…”

Section: Elegans Dauers Have Attenuated Responses To Adult Odors 579mentioning

confidence: 99%

“…To determine how the Daf-c mutants interact with chemosensory mutants 284 mediating the ZTDO response, we examined the chemotaxis behavior of the Daf-285 c mutants in the loss-of-function obi-1(tu404) background that is defective for its 286 cGMP-dependent responses to ZTDO [68]. We found that the obi-1 background 287 did not affect ZTDO attraction in both tu391 adults and DL ( Fig.…”

Section: Introduction 36mentioning

confidence: 99%

“…To better define the genetic pathways that are responsible for the 267 chemosensory differences between DL and adults, we focused on the responses 268 of the Daf-c mutants to the Oriental beetle pheromone ZTDO [68]. We observed 269 that wild-type PS312 showed the strongest difference between the DL and adults, 270…”

Section: Introduction 36mentioning

confidence: 99%

“…3B). We were unable to perform chemotaxis assays on the DL 291 of obi-1(tu404) mutants due to its high DL mortality rate, especially in the presence 292 of ZTDO [68]. Interestingly, while Ppa-daf-12 is epistatic to csu60 for dauer 293 formation, csu60; daf-12(tu389) double mutant adults still exhibited the csu60 294 enhanced attraction to ZTDO ( Fig.…”

Section: Introduction 36mentioning

confidence: 99%

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The Regulation of Developmental Diapause is Coordinated with Molting and Olfaction

Carstensen

Villalon

Vertiz

et al. 2020

Preprint

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1Developmental and behavioral plasticity allow animals to prioritize alternative 2 genetic programs during fluctuating environments. Behavioral remodeling may be 3 acute in animals that interact with host organisms, since reproductive adults and 4 the developmentally arrested larvae often have different ethological needs for 5 chemical stimuli. To understand the genes that coordinate development and 6 behavior, we used the nematode model Pristionchus pacificus to characterize 7 mutants that inappropriately enter developmental diapause to become dauer 8 larvae (Daf-c). We found several key olfactory differences between P. pacificus 9 and C. elegans Daf-c dauers. In addition, the two P. pacificus Daf-c alleles disrupt 10 steroid synthesis required for proper regulation of the conserved canonical steroid 11 hormone receptor DAF-12, whose dauer-constitutive and cuticle exsheathment 12 phenotypes can be rescued by the feeding of ∆7-dafachronic acid. One allele, 13 csu60, has a deletion in the sole HydroxySteroid Dehydrogenase (HSD) in P. 14 pacificus. Both hsd-2(csu60) adults and dauers show enhanced attraction to a 15 beetle pheromone, possibly due to the heterochronic activation of dauer-specific 16 neuronal development in the adults. Surprisingly, this enhanced odor attraction 17 acts independently of daf-12, revealing unexpected targets of steroid hormones 18 regulating ecdysis and olfaction in P. pacificus. analysis in diverse species of nematodes as a result of comparative studies in non-52Caenorhabditis species [8][9][10]. 53The commitment to dauer entry requires external as well as internal factors. 54Multiple parallel-acting signaling factors in the dauer regulation pathway have been 55 characterized in C. elegans, such as the recognition of pheromones by G-protein-56 coupled receptors in the chemosensory amphid neurons and signal transduction 57 to the DAF-11/guanylyl cyclase pathway [11]. Favorable conditions also lead to 58 signaling in the DAF-7/TGF-ß and DAF-2/insulin-like pathways [11][12][13][14]. This 59 signaling facilitates the production of ∆4-and ∆7-dafachronic acid from the 60 cholesterol precursor, which act as ligands that bind to the highly conserved 61 nuclear hormone receptor DAF-12 and prevent DAF-12 from repressing genes 62 necessary for development to reproductive adulthood [11,[15][16][17]. Less favorable 63 conditions produce pheromones that reduce signaling in these pathways, 64 subsequently shifting DAF-12 from its inactive ligand-bound form to its active, 65ligand-free form that specifies dauer development. Despite being a non-feeding 66 and stress-resistant stage, the DL are actively motile and receptive to external 67 chemosensory and mechanical cues. For both parasitic and free-living nematodes, 68 DL share the ability to survive harsh conditions but remain receptive to 69 semiochemicals from their environment. 70The commitment to dauer also prompts the rewiring of the sensory nervous 71 system in C. elegans [6,18,19], but the molecular basis of this process remains 72 poorly unde...

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Section: Elegans Dauers Have Attenuated Responses To Adult Odors 579mentioning

confidence: 99%

Section: Introduction 36mentioning

confidence: 99%

Section: Introduction 36mentioning

confidence: 99%

Section: Introduction 36mentioning

confidence: 99%

See 2 more Smart Citations

The Regulation of Developmental Diapause is Coordinated with Molting and Olfaction

Carstensen

Villalon

Vertiz

et al. 2020

Preprint

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“…Working with colleagues from two Max Planck Institutes—the MPI for Biology of Ageing and the MPI for Developmental Biology—the CalState researchers have identified a new molecular player in detection of chemical signals. They have also revealed the dual nature of the chemical cue that lures these nematodes to the beetles and, at the same time, arrests their development (Cinkornpumin et al, 2014). …”

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confidence: 99%

Fatal attraction

Ringstad

2014

eLife

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Caenorhabditis elegans dauers vary recovery in response to bacteria from natural habitat

Bubrig

Sutton

Fierst

2020

Ecology and Evolution

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Many species use dormant stages for habitat selection by tying recovery to informative external cues. Other species have an undiscerning strategy in which they recover randomly despite having advanced sensory systems. We investigated whether elements of a species' habitat structure and life history can bar it from developing a discerning recovery strategy. The nematode Caenorhabditis elegans has a dormant stage called the dauer larva that disperses between habitat patches. On one hand, C. elegans colonization success is profoundly influenced by the bacteria found in its habitat patches, so we might expect this to select for a discerning strategy. On the other hand, C. elegans' habitat structure and life history suggest that there is no fitness benefit to varying recovery, which might select for an undiscerning strategy. We exposed dauers of three genotypes to a range of bacteria acquired from the worms' natural habitat. We found that C. elegans dauers recover in all conditions but increase recovery on certain bacteria depending on the worm's genotype, suggesting a combination of undiscerning and discerning strategies. Additionally, the worms' responses did not match the bacteria's objective quality, suggesting that their decision is based on other characteristics.

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A host beetle pheromone regulates development and behavior in the nematode Pristionchus pacificus

Cited by 32 publications

References 53 publications

The Regulation of Developmental Diapause is Coordinated with Molting and Olfaction

The Regulation of Developmental Diapause is Coordinated with Molting and Olfaction

Fatal attraction

Caenorhabditis elegans dauers vary recovery in response to bacteria from natural habitat

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