Acute growth hormone administration increases myoglobin expression and Glut4 translocation in rat cardiac muscle cells

Sertié, Rogério Antônio Laurato; Sertié, Andréa L.; Giannocco, Gisele; Poyares, L. L.; Nunes, Maria Tereza

doi:10.1016/j.metabol.2014.08.012

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…The nitrocellulose membrane was incubated with human anti myoglobin antiserum (1/5000; Sigma. Modified from Giannocco et al 2004 andSertié et al 2014), diluted in blocking buffer overnight at 4°C and washed for 30 min with the blocking buffer without milk. The membranes were, then, incubated with secondary antibody in blocking buffer (137 mM NaCl, 2.7 mM KCl, 8 mM NaHPO 4 , 1.4 mM KH 2 PO 4 , and 0.02% Tween 20) for 1 h at room temperature and then washed again for 30 min as described above (Maniatis et al 1989).…”

Section: Race-pat (Rapid Amplification Of Cdna Endspoly(a) Test) Formentioning

confidence: 99%

Iron restriction increases myoglobin gene and protein expression in Soleus muscle of rats

Souza

Brunetto

Nunes

2016

An. Acad. Bras. Ciênc.

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Iron is an important trace element for proper cell functioning. It is present in cytochromes, hemoglobin and myoglobin (Mb), where it binds to oxygen. It is also an electron acceptor in the respiratory chain. Mb is an 18 kDa heme-protein, highly expressed in skeletal muscle and heart. The expression of several genes involved in the metabolism of iron is post-transcriptionally regulated by this element. Iron was shown to interfere with the polyadenylation step, modifying their poly (A) tail length and, as a consequence, their stability and translation rate. The aim of this study was to investigate whether iron supplementation or long and short-term restriction affects Mb gene and protein expression, as well as Mb mRNA poly(A) tail length, in cardiac and skeletal muscles of rats. Long-term iron restriction caused an increase in Mb gene and protein expression in Soleus muscle. No changes were observed in extensor digitorum longus muscle and heart. Short-term iron supplementation after iron deprivation did not alter Mb gene expression and mRNA poly(A) tail length in all tissues studied. These results indicate that Mb gene and protein expression is upregulated in response to iron deprivation, an effect that is tissue-specific and seems to occur at transcriptional level.

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Section: Race-pat (Rapid Amplification Of Cdna Endspoly(a) Test) Formentioning

confidence: 99%

Iron restriction increases myoglobin gene and protein expression in Soleus muscle of rats

Souza

Brunetto

Nunes

2016

An. Acad. Bras. Ciênc.

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“…In this regard, only plasma membrane expression of GLUT4, GLUT8, and GLUT12 is known to be governed by insulin ( Shao and Tian, 2015 ). For the other GLUT isotypes, also other factors, such as other hormones and also ions, can control the translocation ( Rattigan et al, 1991 ; Benomar et al, 2006 ; Martinez et al, 2014 ; Sertié et al, 2014 ; Gil-Iturbe et al, 2019 ; Burkuš et al, 2020 ). There are important species differences with respect to GLUT expression.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of training and acute exercise-induced shifts in muscular glucose transporter (GLUT) 4, 8, and 12 expression in locomotion versus posture muscles in healthy horses

Vidal Moreno de Vega,

Lemmens,

de Meeûs d’Argenteuil

et al. 2023

Front. Physiol.

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Important changes in glucose transporter (GLUT) expression should be expected if the glucose influx plays a pivotal role in fuelling or connecting metabolic pathways that are upregulated in response to exercise. The aim was to assess GLUT4, 8, and 12 dynamics in response to training and acute exercise.Methods: Sixteen untrained Standardbred mares (3-4 year) performed an incremental SET at the start and end of 8 weeks harness training. M. pectoralis (PM) and M. vastus lateralis (VL) muscle biopsies were taken before and after each SET, allowing for comparing rest and acute samples in untrained (UT) and trained (T) condition using Western Blot for GLUT quantification and Image Pro v.10 for Blot analysis. Data were normalized against GAPDH. Basal GLUT-levels of PM versus VL were analysed with the Wilcoxon matched-pairs signed rank test. The effect of acute exercise or training was assessed using the Friedman test with a post hoc Dunn’s.Results: Basal GLUT4 and GLUT12 protein expression were significantly higher in the VL compared to the PM (PGLUT4 = 0.031 and PGLUT12 = 0.002). Training had no effect on basal GLUT4 expression, neither in the VL (p > 0.9999), nor the PM (p > 0.9999). However, acute exercise in trained condition significantly decreased GLUT4 expression in the VL (p = 0.0148). Neither training nor acute exercise significantly changed total GLUT8 protein expression. Training significantly decreased total GLUT12 protein expression in rest biopsies, only visible in the VL (p = 0.0359). This decrease was even more prominent in the VL after acute exercise in trained condition (PVL = 0.0025).Conclusion: The important changes seen in GLUT12 expression downregulation, both in response to training and acute exercise in the horse, the downregulation of GLUT4 expression after acute exercise in trained condition and the lack of differential shifts in GLUT8 expression in any of the studied conditions, questions the importance of glucose as substrate to fuel training and exercise in healthy horses. These findings encourage to further explore alternative fuels for their involvement in equine muscular energetics.

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Muscle and skeletal health in children and adolescents with GH deficiency

Improda

Capalbo

Esposito

et al. 2016

Best Practice & Research Clinical Endocrinology & Metab

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Acute growth hormone administration increases myoglobin expression and Glut4 translocation in rat cardiac muscle cells

Abstract: These results suggest that GH exerts some of its effects on cardiomyocytes shortly after the first administration inducing the expression of proteins potentially involved in cardiac performance.

Cited by 5 publications

References 19 publications

Iron restriction increases myoglobin gene and protein expression in Soleus muscle of rats

Iron restriction increases myoglobin gene and protein expression in Soleus muscle of rats

Dynamics of training and acute exercise-induced shifts in muscular glucose transporter (GLUT) 4, 8, and 12 expression in locomotion versus posture muscles in healthy horses

Muscle and skeletal health in children and adolescents with GH deficiency

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