Effect of Swimming on the Production of Aldosterone in Rats

Abstract

It has been demonstrated that exercise is one of the stresses known to increase the aldosterone secretion. Both potassium and angiotensin II (Ang II) levels are shown to be correlated with aldosterone production during exercise, but the mechanism is still unclear. In an in vivo study, male rats were catheterized via right jugular vein (RJV), and divided into four groups namely water immersion, swimming, lactate infusion (13 mg/kg/min) and pyruvate infusion (13 mg/kg/min) groups. Each group was treated for 10 min. Blood samples were collected at 0, 10, 15, 30, 60 and 120 min from RJV after administration. In an in vitro study, rat zona glomerulosa (ZG) cells were challenged by lactate (1–10 mM) in the presence or absence of Ang II (10−8 M) for 60 min. The levels of aldosterone in plasma and medium were measured by radioimmunoassay. Cell lysates were analyzed by immunoblotting assay. After exercise and lactate infusion, plasma levels of aldosterone and lactate were significantly higher than those in the control group. Swimming for 10 min significantly increased the plasma Ang II levels in male rats. Administration of lactate plus Ang II significantly increased aldosterone production and enhanced protein expression of steroidogenic acute regulatory protein (StAR) in ZG cells. These results demonstrated that acute exercise led to the increase of both aldosterone and Ang II secretion, which is associated with lactate action on ZG cells and might be dependent on the activity of renin-angiotensin system.

Influence of Sodium Citrate Supplementation after Dehydrating Exercise on Responses of Stress Hormones to Subsequent Endurance Cycling Time-Trial in the Heat

“…Plasma ALDO and K + concentrations did not change in NaHCO 3 trial, but in KHCO 3 trial, parallel increases in ALDO and K + levels occurred [42]. Considering these data [38,39,40,41,42], it seems unlikely that reduced ALDO levels were induced by metabolic alkalosis in our participants in CIT trial.…”

“…In goats, metabolic alkalosis suppressed blood ALDO concentration in the absence of detectable changes in plasma K + concentration or renin activity [38], which are considered primary regulators of ALDO secretion [39,40]. However, in sedentary rats, alkalosis did not decrease, but rather increased plasma ALDO concentration [41]. In young men, ingestion of either NaHCO 3 or KHCO 3 induced metabolic alkalosis, but neither of these substances suppressed plasma ALDO levels [42].…”

Adrenal stress hormone action in skeletal muscle during exercise training: An old dog with new tricks?

“…Though aldosterone is secreted upon initiation of exercise, 167 evidence for a role in mediating skeletal muscle adaptations and changes in performance in healthy individuals is purely speculative at the current time because of a lack of data. In fact, data in elderly or diseased populations imply that chronic elevations in aldosterone may actually be detrimental for skeletal muscle health [168][169][170][171] ( Figure 6A).…”

Downregulation of testosterone production through luteinizing hormone receptor regulation in male rats exposed to 17α-ethynylestradiol

“…Lab. Ltd.), as described previously 55,56 . The catheter was passed subcutaneously towards the nape, where it was allowed to protrude through a small incision.…”