Presence of Potential Toxin-Producing Cyanobacteria in an Oligo-Mesotrophic Lake in Baltic Lake District, Germany:  An Ecological, Genetic and Toxicological Survey

Dadheech, Pawan K.; Selmeczy, Géza B.; Vasas, Gábor; Padisák, Judit; Arp, W.; Tapolczai, Kálmán; Casper, Peter; Krienitz, Lothar

doi:10.3390/toxins6102912

Cited by 22 publications

(18 citation statements)

References 65 publications

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“…was the only microcystin producer present. Those findings are in line with other studies, which found that the dominant microcystin producer is not necessarily the dominant cyanobacterial species in a water body ( Dadheech et al, 2014 ). Similar to our study, Lee et al (2015) identified low abundant Microcystis sp.…”

Section: Discussionsupporting

confidence: 93%

Temporal Dynamics of the Microbial Community Composition with a Focus on Toxic Cyanobacteria and Toxin Presence during Harmful Algal Blooms in Two South German Lakes

Scherer

Millard

Miller³

et al. 2017

Front. Microbiol.

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Bacterioplankton plays an essential role in aquatic ecosystems, and cyanobacteria are an influential part of the microbiome in many water bodies. In freshwaters used for recreational activities or drinking water, toxic cyanobacteria cause concerns due to the risk of intoxication with cyanotoxins, such as microcystins. In this study, we aimed to unmask relationships between toxicity, cyanobacterial community composition, and environmental factors. At the same time, we assessed the correlation of a genetic marker with microcystin concentration and aimed to identify the main microcystin producer. We used Illumina MiSeq sequencing to study the bacterioplankton in two recreational lakes in South Germany. We quantified a microcystin biosynthesis gene (mcyB) using qPCR and linked this information with microcystin concentration to assess toxicity. Microcystin biosynthesis gene (mcyE)-clone libraries were used to determine the origin of microcystin biosynthesis genes. Bloom toxicity did not alter the bacterial community composition, which was highly dynamic at the lowest taxonomic level for some phyla such as Cyanobacteria. At the OTU level, we found distinctly different degrees of temporal variation between major bacteria phyla. Cyanobacteria and Bacteroidetes showed drastic temporal changes in their community compositions, while the composition of Actinobacteria remained rather stable in both lakes. The bacterial community composition of Alpha- and Beta-proteobacteria remained stable over time in Lake Klostersee, but it showed temporal variations in Lake Bergknappweiher. The presence of potential microcystin degraders and potential algicidal bacteria amongst prevalent Bacteroidetes and Alphaproteobacteria implied a role of those co-occurring heterotrophic bacteria in cyanobacterial bloom dynamics. Comparison of both lakes studied revealed a large shared microbiome, which was shaped toward the lake specific community composition by environmental factors. Microcystin variants detected were microcystin-LR, -RR, and -YR. The maximum microcystin concentrations measured was 6.7 μg/L, a value still acceptable for recreational waters but not drinking water. Microcystin concentration correlated positively with total phosphorus and mcyB copy number. We identified low abundant Microcystis sp. as the only microcystin producer in both lakes. Therefore, risk assessment efforts need to take into account the fact that non-dominant species may cause toxicity of the blooms observed.

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Section: Discussionsupporting

confidence: 93%

Temporal Dynamics of the Microbial Community Composition with a Focus on Toxic Cyanobacteria and Toxin Presence during Harmful Algal Blooms in Two South German Lakes

Scherer

Millard

Miller³

et al. 2017

Front. Microbiol.

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“…The absence of Anatoxin-a genes in the studied lake is in agreement with previous screening for a toxin screening in the lake [77].…”

Section: Discussionsupporting

confidence: 90%

Recovering genomic clusters of secondary metabolites from lakes: a Metagenomics 2.0 approach

Cuadrat

Ionescu

Dávila

et al. 2017

Preprint

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BackgroundMetagenomic approaches became increasingly popular in the past decades due to decreasing costs of DNA sequencing and bioinformatics development. So far, however, the recovery of long genes coding for secondary metabolism still represents a big challenge. Often, the quality of metagenome assemblies is poor, especially in environments with a high microbial diversity where sequence coverage is low and complexity of natural communities high. Recently, new and improved algorithms for binning environmental reads and contigs have been developed to overcome such limitations. Some of these algorithms use a similarity detection approach to classify the obtained reads into taxonomical units and to assemble draft genomes. This approach, however, is quite limited since it can classify exclusively sequences similar to those available (and well classified) in the databases.In this work, we used draft genomes from Lake Stechlin, north-eastern Germany, recovered by MetaBat, an efficient binning tool that integrates empirical probabilistic distances of genome abundance, and tetranucleotide frequency for accurate metagenome binning. These genomes were screened for secondary metabolism genes, such as polyketide synthases (PKS) and non-ribosomal peptide synthases (NRPS), using the Anti-SMASH and NAPDOS workflows.ResultsWith this approach we were able to identify 243 secondary metabolite clusters from 121 genomes recovered from the lake samples. A total of 18 NRPS, 19 PKS and 3 hybrid PKS/NRPS clusters were found. In addition, it was possible to predict the partial structure of several secondary metabolite clusters allowing for taxonomical classifications and phylogenetic inferences.ConclusionsOur approach revealed a great potential to recover and study secondary metabolites genes from any aquatic ecosystem.

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“…S4), while there were several taxa of equal abundance in summer including C. meneghiniana , Cyclostephanos, Chlamydomonas , Nitzschia, Coelastrum , Navicula , and Scenedesmus . Cyanobacteria, including Oscillatoria, Pseudanabaena , and Planktothrix sp., with the latter being a well‐known toxin producer in temperate lakes (Ernst et al ; Dadheech et al ), were present in low relative abundance. Community composition in summer was more diverse than in spring (Fig.…”

Section: Resultsmentioning

confidence: 99%

Structural changes of the microplankton community following a pulse of inorganic nitrogen in a eutrophic river

Kleinteich

Hilt

Hoppe

et al. 2019

Limnology & Oceanography

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Global change will increase the number and severity of extreme events resulting in strongly pulsed nutrient loading to rivers. Recent studies indicate the potential for rapid plankton shifts as short-term response to storm events and highlight the need for high-frequency methods to understand these complex processes. Here, we studied the effects of a strong short-term pulse of ammonium nitrate in a eutrophic temperate river that elevated nitrate-N concentrations from 3.1 to 10-55 mg L −1 . An intense phytoplankton bloom increased chlorophyll a concentrations from below 10 μg L −1 to over 140 μg L −1 despite of high background N concentrations and followed the N-pulse downstream. High-throughput sequencing revealed the temporal dynamics of the bacterial (16S rRNA gene) and microeukaryotic (18S rRNA gene) community throughout the event. Bacterial and microeukaryotic community richness was reduced, and community composition changed significantly during the bloom. Few microeukaryote taxa (e.g., Cyclotella meneghiniana, Chlamydomonas) dominated the bloom and replaced the rich summer phytoplankton community. Long-term monitoring data (2008-2016) using classical microscope analyses showed that seasonal shifts in phytoplankton community composition were linked to physical parameters and nutrient availability with P-limitation observed in spring but not in summer. We conclude that a disturbance in abiotic and biotic control factors, such as caused by a nutrient pulse, can induce structural shifts in the microplankton community leading to a phytoplankton bloom, even in eutrophic waters.

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Presence of Potential Toxin-Producing Cyanobacteria in an Oligo-Mesotrophic Lake in Baltic Lake District, Germany: An Ecological, Genetic and Toxicological Survey

Cited by 22 publications

References 65 publications

Temporal Dynamics of the Microbial Community Composition with a Focus on Toxic Cyanobacteria and Toxin Presence during Harmful Algal Blooms in Two South German Lakes

Temporal Dynamics of the Microbial Community Composition with a Focus on Toxic Cyanobacteria and Toxin Presence during Harmful Algal Blooms in Two South German Lakes

Recovering genomic clusters of secondary metabolites from lakes: a Metagenomics 2.0 approach

Structural changes of the microplankton community following a pulse of inorganic nitrogen in a eutrophic river

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