PEGylated silver doped zinc oxide nanoparticles as novel photosensitizers for photodynamic therapy against Leishmania

Nadhman, Akhtar; Nazir, Samina; Khan, Mazhar; Arooj, Syeda; Muhammad, Bakhtiar; Shahnaz, Gul; Yasinzai, Masoom

doi:10.1016/j.freeradbiomed.2014.09.005

Cited by 96 publications

(59 citation statements)

References 40 publications

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“…ZNPs have also been reported to have antimicrobial activities. 7 In addition, Nadhman et al 8 used ZNPs as novel photosensitizers for photodynamic therapy against Leishmania.…”

Section: Introductionmentioning

confidence: 99%

Anticoccidial and antioxidant activities of zinc oxide nanoparticles on Eimeria papillata-induced infection in the jejunum

Dkhil¹,

Al‐Quraishy²,

Wahab³

2015

IJN

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Nanomedicine has recently emerged as a better option for the treatment of various diseases. Here, we investigated the in vivo anticoccidial properties of zinc oxide nanoparticles (ZNPs). ZNPs were crystalline in nature, with a smooth and spherical surface and a diameter in the range of ~10–15 nm. The X-ray diffraction pattern was utilized to identify the crystalline property of the grown ZNPs, whereas field emission scanning electron microscopy was employed to check the size and morphology of the ZNPs. The data showed that mice infected with Eimeria papillata produced 29.7×10 3 ±1,500 oocysts/g feces on day 5 postinfection. This output was significantly decreased, to 12.5×10 3 ±1,000 oocysts, in mice treated with ZNPs. Infection also induced inflammation and injury of the jejunum. This was evidenced (1) through an increase in the inflammatory histological score, (2) through increased production of nitric oxide and malondialdehyde, and (3) through a decrease in both the glutathione level and goblet cell number in mice jejuna. All these infection-induced parameters were significantly altered during treatment with ZNPs. Our results indicate, therefore, that ZNPs have protective effects against E. papillata -induced coccidiosis.

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“…ZNPs have also been reported to have antimicrobial activities. 7 In addition, Nadhman et al 8 used ZNPs as novel photosensitizers for photodynamic therapy against Leishmania.…”

Section: Introductionmentioning

confidence: 99%

Anticoccidial and antioxidant activities of zinc oxide nanoparticles on Eimeria papillata-induced infection in the jejunum

Dkhil¹,

Al‐Quraishy²,

Wahab³

2015

IJN

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“…Similarly, with Ag as dopant of the semiconductor, nanoparticles of zinc oxide demonstrated the production of ROS when activated by daylight and were able to kill L. tropica. 139 More recently, two approaches were combined, using AmpB adsorbed on preformed spherical biogenic silver nanoparticles surface. AmB-Ag nanoparticles were more potent in inhibiting L. tropica in in vitro tests.…”

Section: Bruni Et Almentioning

confidence: 99%

Nanostructured delivery systems with improved leishmanicidal activity: a critical review

Bruni¹,

Stella²,

Giraudo³

et al. 2017

IJN

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Leishmaniasis is a vector-borne zoonotic disease caused by protozoan parasites of the genus Leishmania , which are responsible for numerous clinical manifestations, such as cutaneous, visceral, and mucocutaneous leishmaniasis, depending on the site of infection for particular species. These complexities threaten 350 million people in 98 countries worldwide. Amastigotes living within macrophage phagolysosomes are the principal target of antileishmanial treatment, but these are not an easy target as drugs must overcome major structural barriers. Furthermore, limitations on current therapy are related to efficacy, toxicity, and cost, as well as the length of treatment, which can increase parasitic resistance. Nanotechnology has emerged as an attractive alternative as conventional drugs delivered by nanosized carriers have improved bioavailability and reduced toxicity, together with other characteristics that help to relieve the burden of this disease. The significance of using colloidal carriers loaded with active agents derives from the physiological uptake route of intravenous administered nanosystems (the phagocyte system). Nanosystems are thus able to promote a high drug concentration in intracellular mononuclear phagocyte system (MPS)-infected cells. Moreover, the versatility of nanometric drug delivery systems for the deliberate transport of a range of molecules plays a pivotal role in the design of therapeutic strategies against leishmaniasis. This review discusses studies on nanocarriers that have greatly contributed to improving the efficacy of antileishmaniasis drugs, presenting a critical review and some suggestions for improving drug delivery.

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“…Human macrophages were isolated by the Ficoll-Gastrografin ® method and their biocompatibility was tested and calculated using a previously used method of Nadhman et al 15 …”

Section: Biocompatibility Of Synthesized Nanoparticles Human Macrophagesmentioning

confidence: 99%

“…The experiments were carried out by our previously used method 15 with modifications. Briefly, stock solutions of 1 mg/mL of the different ZnCuO particles were dissolved in deionized water.…”

Section: Hemolysis Assaymentioning

confidence: 99%

“…13 This study focuses on the development of six biocompatible nanoparticles of ZnO doped with different concentrations of Cu (1%, 5%, and 10%, ZnCuO1-ZnCuO3) and 5% N with Cu (1%, 5%, and 10%, ZnCuO4-ZnCuO6, respectively) and their in vitro efficacy against Leishmania parasite. Previously, we had used silver as a dopant 15 but it produced a lower ROS, and the half maximal inhibitory concentration (IC 50 ) against Leishmania cells was not efficient as compared to the currently synthesized ZnCuO nanoparticles. In the current study, Cu and Cu-N were selected as dopants as they cause multi-electron reduction of oxygen, which produce a higher quantity of lethal ROS in the immediate environment upon exposure to visible portion of light, and thus were more effective photodynamic killers.…”

Section: Introductionmentioning

confidence: 99%

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Visible-light-responsive ZnCuO nanoparticles: benign photodynamic killers of infectious protozoans

Nadhman¹,

Nazir²,

Khan³

et al. 2015

IJN

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Human beings suffer from several infectious agents such as viruses, bacteria, and protozoans. Recently, there has been a great interest in developing biocompatible nanostructures to deal with infectious agents. This study investigated benign ZnCuO nanostructures that were visible-light-responsive due to the resident copper in the lattice. The nanostructures were synthesized through a size-controlled hot-injection process, which was adaptable to the surface ligation processes. The nanostructures were then characterized through transmission electron microscopy, X-ray diffraction, diffused reflectance spectroscopy, Rutherford backscattering, and photoluminescence analysis to measure crystallite nature, size, luminescence, composition, and band-gap analyses. Antiprotozoal efficiency of the current nanoparticles revealed the photodynamic killing of Leishmania protozoan, thus acting as efficient metal-based photosensitizers. The crystalline nanoparticles showed good biocompatibility when tested for macrophage toxicity and in hemolysis assays. The study opens a wide avenue for using toxic material in resident nontoxic forms as an effective antiprotozoal treatment.

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PEGylated silver doped zinc oxide nanoparticles as novel photosensitizers for photodynamic therapy against Leishmania

Cited by 96 publications

References 40 publications

Anticoccidial and antioxidant activities of zinc oxide nanoparticles on Eimeria papillata-induced infection in the jejunum

Anticoccidial and antioxidant activities of zinc oxide nanoparticles on Eimeria papillata-induced infection in the jejunum

Nanostructured delivery systems with improved leishmanicidal activity: a critical review

Visible-light-responsive ZnCuO nanoparticles: benign photodynamic killers of infectious protozoans

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