Integration of genomic information in the clinical management of HCC

Quetglas, I.M.; Moeini, Agrin; Pinyol, Roser; Llovet, Josep M.

doi:10.1016/j.bpg.2014.08.004

Cited by 20 publications

(16 citation statements)

References 95 publications

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“…Precision medicine was first practiced in oncology because of its potential to enhance targeted cancer therapies 2 . Precision medicine focuses on the following four significant aspects: human genetic information, transcription, protein and function/activity 3, 4 . Although defining genetic information and its transcription is quite common and easy, proteins and their activities should be determined to understand their biological in case of changes in their functions 5 .…”

Section: Introductionmentioning

confidence: 99%

LC-MS/MS quantification of sulfotransferases is better than conventional immunogenic methods in determining human liver SULT activities: implication in precision medicine

Xie

Yan

Jiamei

et al. 2017

Sci Rep

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This study aims to determine whether enzyme activities are correlated with protein amounts and mRNA expression levels of five major human sulfotransferase (SULT) enzymes in 10 matched pericarcinomatous and hepatocellular carcinoma liver samples. The MRM UHPLC-MS/MS method, Western blot and RT-PCR were used along with SULT activity measurement using probe substrates. The LC-MS/MS method was specific for all five tested SULTs, whereas Western blot was specific for only two isoforms. The activities of SULT1A1, SULT1B1, SULT1E1 and SULT2A1 in 9 of 10 samples showed a significant decrease in tumor tissues relative to matched pericarcinomatous tissues, whereas the activities of SULT1A3 in 7 of 10 samples increased. The turnover numbers of SULTs did not change, except for SULT1A1. A generally high degree of correlations was observed between SULT activities and protein amounts (r2 ≥ 0.59 except one), whereas a low degree of correlations was observed between SULT activities and mRNA expression levels (r2 ≤ 0.48 except one). HCC reduced the SULT activities via impaired protein amounts. LC-MS/MS quantification of SULTs is highly reliable measurement of SULT activities, and may be adopted for implementing precision medicine with respect to drugs mainly metabolized by SULTs in healthy and HCC patients.

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Section: Introductionmentioning

confidence: 99%

LC-MS/MS quantification of sulfotransferases is better than conventional immunogenic methods in determining human liver SULT activities: implication in precision medicine

Xie

Yan

Jiamei

et al. 2017

Sci Rep

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“…Thus, assaying telomere length can serve as a biomarker for diverse tumors, including those involving solid organs like the liver [5–7]. …”

Section: Introductionmentioning

confidence: 99%

Paired assessment of liver telomere lengths in hepatocellular cancer is a reliable predictor of disease persistence

Feng

et al. 2017

Bioscience Reports

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In the present study, we used a small series of highly defined patients, where we had matched timed peripheral blood samples (PBS), as well as paired liver biopsies obtained during collection of blood samples from patients with diagnosed hepatocellular carcinoma (HCC) and compared the correlation between the changes of telomere lengths in these defined samples. Patients included had either HCC alone or in conjunction with either pre-existing hepatitis B virus (HBV) or hepatitis C virus (HCV) infection. PCR-based assay incorporating primers to the telomeric hexamer repeats to polymerize and detect telomeric DNA was used. The average telomere length for each independent assessment was measured by seeing the differences in the intensity of the sample’s telomere signal (T) to the signal from a single-copy gene (S-, β-globin) to estimate the standard ratio. Our results provide the first convincing evidence that PBS may be utilized to assay telomere shortening as a predictor for disease persistence in HCC resulting after HBV or HCV infection, but not in non-infectious cause-stimulated HCC. These findings provide incipient opportunity to develop telomere length assessment as a biomarker tool for prediction of HCC in patients with HBV or HCV infection, as well as to gauge responses to chemotherapy and other treatment modalities.

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“…With a high death rate attributed to high mortality and unsatisfactory treatment options, hepatocellular carcinoma (HCC) is one of the most prevalent malignant cancers worldwide [1][2][3]. So effective therapeutic tools, such as gene therapy, should be developed for HCC treatment.…”

Section: Introductionmentioning

confidence: 99%

miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression

Peng

You

et al. 2015

Experimental Cell Research

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MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression at post-transcriptional level. miRNA dysregulation plays a causal role in cancer progression. In this study, miR-208-3p was highly expressed and directly repressed ARID2 expression. As a result, ARID2 expression in hepatocellular carcinoma (HCC) was decreased. In vitro, miR-208-3p down-regulation and ARID2 over-expression elicited similar inhibitory effects on HCC cell proliferation and invasion. In vivo test results revealed that miR-208-3p down-regulation inhibited HCC tumorigenesis in Hep3B cells. Moreover, ARID2 was possibly a downstream element of transforming growth factor beta1 (TGFβ1)/miR-208-3p/ARID2 regulatory pathway. These findings suggested that miR-208-3p up-regulation is associated with HCC cell progression and may provide a new target for liver cancer treatment.

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Integration of genomic information in the clinical management of HCC

Cited by 20 publications

References 95 publications

LC-MS/MS quantification of sulfotransferases is better than conventional immunogenic methods in determining human liver SULT activities: implication in precision medicine

LC-MS/MS quantification of sulfotransferases is better than conventional immunogenic methods in determining human liver SULT activities: implication in precision medicine

Paired assessment of liver telomere lengths in hepatocellular cancer is a reliable predictor of disease persistence

miR-208-3p promotes hepatocellular carcinoma cell proliferation and invasion through regulating ARID2 expression

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