Development of 12 novel polymorphic microsatellite markers using a next generation sequencing approach for Spiculopteragia spiculoptera, a nematode parasite of deer

Patrelle, Cécile; Jouet, Damien; Lehrter, Véronique; Ferté, Hubert

doi:10.1016/j.molbiopara.2014.09.004

Cited by 1 publication

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References 33 publications

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“…(Criscione and Blouin, 2007). Despite their potential utility, microsatellites as genetic markers for parasitic nematodes have been not much explored (Johnson et al ., 2006; Criscione et al ., 2007; Redman et al ., 2008; Temperley et al ., 2009; Betson et al ., 2011; Reid et al ., 2012; Patrelle et al ., 2014; Rabelo et al ., 2017; Greeff et al ., 2018). This may be explained by some complexity when using microsatellites, which could arise from potential homoplasy, i.e.…”

Section: Discussionmentioning

confidence: 99%

Novel polymorphic microsatellite loci inAnisakis pegreffiiandA. simplex(s. s.) (Nematoda: Anisakidae): implications for species recognition and population genetic analysis

et al. 2019

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The species of Anisakis constitute one of the most widespread groups of ascaridoid nematodes in the marine ecosystem. Three closely related taxa are recognised in the A. simplex (s. l.) complex, i.e. A. pegreffii, A. simplex (s. s.) and A. berlandi. They are distributed in populations of their intermediate/paratenic (fish and squids) and definitive (cetaceans) hosts. A panel of seven microsatellite loci (Anisl 05784, Anisl 08059, Anisl 00875, Anisl 07132, Anisl 00314, Anisl 10535 and Anisl 00185), were developed and validated on a total of N = 943 specimens of A. pegreffii and A. simplex (s. s.), collected in fish and cetacean hosts from allopatric areas within the range of distribution of these parasite species. In addition, the locus Anisl 7, previously detected in those Anisakis spp., was investigated. The parasites were first identified by sequence analysis of the EF1 α-1 nDNA. The panel of the microsatellites loci here developed have allowed to: (i) detect diagnostic microsatellite loci between the two species; (ii) identify specimens of the two species A. pegreffii, A. simplex (s. s.) in a multi-marker nuclear genotyping approach; (iii) discover two sex-linked loci in both Anisakis species and (iv) estimate levels of genetic differentiation at both the inter- and intra-specific level.

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