The role of β2-glycoprotein I (β2GPI) carbohydrate chains in the reactivity of anti-β2GPI antibodies from patients with primary antiphospholipid syndrome and in the activation and differentiation of U937 cells

Hernández‐Ramírez, Diego F.; Olivares-Martínez, Elizabeth; Núñez‐Álvarez, Carlos A.; Chavelas, Eneas A.; García‐Hernández, Enrique; Gómez-Hernández, Gregoria; Llórente, Luis; Cabral, Antonio R.

doi:10.1016/j.bbrc.2014.09.064

Cited by 4 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human β2-GP I is a glycosylated protein present in plasma. It has been reported that the affinity of aβ2-GP I against partially glycosylated β2-GP I and completely de-glycosylated β2-GPI is increased compared with native β2-GP I ( 15 ). Based on these results, the present study used a prokaryotic expression system for obtaining β2-GP I protein to ensure lack of glycosylation.…”

Section: Resultsmentioning

confidence: 99%

Effect and mechanism of the aβ2‑GP I/rhβ2‑GP I complex on JEG‑3 cell proliferation, migration and invasion

Ren

Zhang

et al. 2018

Mol Med Report

View full text Add to dashboard Cite

Antiphospholipid antibody (aPL)-mediated antiphospholipid syndrome (APS) is an autoimmune disease. Upon binding to aPL, the primary antigen of aPL, β2-glycoprotein I (β2-GP I), induces abnormal immune function, which further activates downstream signaling pathways in the cell and eventually leads to APS. The present study aimed to determine whether β2-GP I antigen and anti-β2-glycoprotein I antibody (aβ2-GP I), which belong to the aPL class of antibodies, may affect human chorionic epithelium cell (JEG-3) proliferation, migration and invasion. Recombinant human (rh)β2-GP I protein was expressed using a prokaryotic expression system and aβ2-GP I antibody was purified from the blood serum of 10 patients with recurrent pregnancy loss. JEG-3 cells were stimulated with rhβ2-GP I and aβ2-GP I separately or simultaneously, and serum immunoglobulin G of normal pregnant women was used as negative control. Using cell counting kit-8, cell cycle and transwell assays in addition to EdU staining, it was determined that aβ2-GP I/rhβ2-GP I complex markedly increased JEG-3 cell proliferation, migration and invasion. The results revealed that mRNA levels of inhibitor of nuclear factor (NF)-κB kinase subunit (IKKβ), myeloid differentiation primary response protein MyD88 (MyD88), NF-κB and NF-κB inhibitor α (IκBα), as well as the protein levels of MyD88, IκBα and phospho(p)-IκBα in JEG-3 cells increased following incubation with the aβ2-GP I/rhβ2-GP I complex. The observed upregulation of p-IκBα protein suggested that IκBα-mediated inhibition of NF-κB was weakened. Furthermore, JEG-3 cells were transfected with PGMLV-NF-κB-Lu vector. Luciferase activity in JEG-3-NFκB-Luc1 and JEG-3-NFκB-Luc2 cells was enhanced following treatment with aβ2-GP I/rhβ2-GP I complex. The present study demonstrated that aβ2-GP I/rhβ2-GP I complex activates NF-κB through MyD88 signal transduction pathway, which further enhances JEG-3 cell proliferation, migration and invasion.

show abstract

Section: Resultsmentioning

confidence: 99%

Effect and mechanism of the aβ2‑GP I/rhβ2‑GP I complex on JEG‑3 cell proliferation, migration and invasion

Ren

Zhang

et al. 2018

Mol Med Report

View full text Add to dashboard Cite

show abstract

“…Variations of the carbohydrate chains of β2-GPI correlate to some clinical manifestations [52] [53]. The pathogenic key role of the β2-GPI domain I is further highlighted by the report, in a β2-GPI immunized mouse-model of APS, of a better oral tolerance when mice were fed with β2-GPI-D1 instead of complete β2-GPI or with β2-GPI-D5 [54].…”

Section: β2-gpi and Cryptic Epitopesmentioning

confidence: 95%

News and meta-analysis regarding anti-Beta 2 glycoprotein I antibodies and their determination

Bettacchioli¹,

Salma²,

Renaudineau

2019

Clinical Immunology

View full text Add to dashboard Cite

Cellular immune response to β2-glycoprotein-I valine/leucine247 phenotypes in Mexican patients with primary antiphospholipid syndrome

et al. 2017

View full text Add to dashboard Cite

The role of β2-glycoprotein I (β2GPI) carbohydrate chains in the reactivity of anti-β2GPI antibodies from patients with primary antiphospholipid syndrome and in the activation and differentiation of U937 cells

Cited by 4 publications

References 38 publications

Effect and mechanism of the aβ2‑GP I/rhβ2‑GP I complex on JEG‑3 cell proliferation, migration and invasion

Effect and mechanism of the aβ2‑GP I/rhβ2‑GP I complex on JEG‑3 cell proliferation, migration and invasion

News and meta-analysis regarding anti-Beta 2 glycoprotein I antibodies and their determination

Cellular immune response to β2-glycoprotein-I valine/leucine247 phenotypes in Mexican patients with primary antiphospholipid syndrome

Contact Info

Product

Resources

About