Follicular fluid Aβ40 concentrations may be associated with ongoing pregnancy following in vitro fertilization

Duan, F.-H.; Chen, Shiling; Chen, Xin; Niu, Jing; Li, Pu; Liu, Yudong; Xu, Lijuan

doi:10.1007/s10815-014-0345-6

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…For example, results of a 2014 study by Gao B found that an ABCB1 blocker inhibited paclitaxel efflux from all ovarian cancer cell lines regardless of ABCB1 expression level, thus indicating that an ABCB1 transporter plays a key role in paclitaxel resistance of ovarian cancer cells. In another study (Gao et al, 2014), Duan et al (2014) found that APP negatively regulated APP precursor protein hydrolysate. A activity during an in vitro fertilization study of plasma amyloid-β 40 regulation of ovarian cancer development (Duan et al, 2014).…”

Section: Molecular Docking Analysismentioning

confidence: 96%

“…In another study (Gao et al, 2014), Duan et al (2014) found that APP negatively regulated APP precursor protein hydrolysate. A activity during an in vitro fertilization study of plasma amyloid-β 40 regulation of ovarian cancer development (Duan et al, 2014). More recently, in 2020, Chulpanova et al confirmed that amniotic fluid-derived mesenchymal stem cells (MSCs) expressing IL-2 could induce ovarian cancer cell apoptosis in vivo (Chulpanova et al, 2020), while the results of another study revealed that high expression of MAPT was linked to poor ovarian cancer prognosis, thus indicating that inhibition of MAPT expression could improve ovarian cancer prognosis (Schroeder et al, 2019).…”

Section: Molecular Docking Analysismentioning

confidence: 96%

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Screening of Chenopodium hybridum L. for Anti-ovarian Cancer Compounds Using UPLC-Q-Exactive MS and Partial Least Squares Analysis

Yang,

Cheng,

Feng

et al. 2023

Pharmacognosy Magazine

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Background Extracts of Chenopodium hybridum L. leaves and stems exerted a significant antiproliferative effect on human A2780 ovarian cancer cells, but C. hybridum active components have not been reported. Materials and Methods Here, a method is described for screening of C. hybridum extracts for potential bioactive components that inhibit A2780 cell proliferation. First, the spectrum–effect relationship between UPLC-Q-Exactive MS chromatograms and C. hybridum extract antiproliferative effect against A2780 cells was established to evaluate extract bioactive components using partial least squares (PLS) analysis. Results The results indicated that the optimal reflux extraction process for preparing C. hybridum extracts with antiproliferative activity involved a suspension of C. hybridum material in 8 volumes of 70% ethanol followed by heating and refluxing twice for 60 min/reflux step and then repeating the extraction and pooling of both the extracts. Chromatographic results revealed five compounds with potential anti-ovarian cancer activities based on inhibition of A2780 cell proliferation: isorhamnetin-3-O-β-D-furanosyl(1↓2)-O-[α-L-rhamnpyranosyl(1↓6)]-β-D-glucopyranoside, kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-pyranoside, kaempferol-3-O-[α-L-rhamnopyranosyl(1″↓2″)]-β-D-galactopyranoside, quercetin-3,7-di-rhamnose, and isorhamnetin-3-acacia disaccharide. Network pharmacological screening revealed nine core cellular targets that potentially interacted with these compounds. Conclusion These results were verified through molecular docking studies that supported the involvement of these compounds in observed C. hybridum A2780 cell antiproliferative effects, thus indicating C. hybridum active components may have value in ovarian cancer treatments.

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Section: Molecular Docking Analysismentioning

confidence: 96%

Section: Molecular Docking Analysismentioning

confidence: 96%

Screening of Chenopodium hybridum L. for Anti-ovarian Cancer Compounds Using UPLC-Q-Exactive MS and Partial Least Squares Analysis

Yang,

Cheng,

Feng

et al. 2023

Pharmacognosy Magazine

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Alzheimer’s disease-related amyloid-β1–42 peptide induces the loss of human sperm function

et al. 2017

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Characteristically identified as the main component of senile plaques present in patients suffering from Alzheimer's disease, Aβ has been detected in human testis and reproductive fluids, but its effect on spermatozoa has not been addressed. The present study evaluated whether the most toxic and aggregant amyloid precursor protein (APP)-proteolytic product, amyloid-β (Aβ), was capable of affecting sperm functionality. Normozoospermic samples were either exposed to different Aβ doses or to the untreated and scrambled controls for a maximum of 48 h at 37 °C and 5%CO, and motility, viability and mitochondrial status were evaluated. Additionally, tyrosine phosphorylation was analyzed by immunocytochemistry and acrosomal integrity through PSA-FITC. A shorter treatment period was used to monitor prompt Ca responses. Aβ peptide decreased motility before inducing mitochondrial impairment (p < 0.05; n = 6). Both outcomes became more pronounced with time, reaching their maximal decrease at 48 h, where even 1 μM produced undesirable effects (p < 0.05; n = 6). Aβ peptide also decreased cell survival (p < 0.05; n = 6). Furthermore, although no effects on tyrosine phosphorylation were observed (p > 0.05; n = 6), reduced acrosomal integrity was detected (p < 0.05; n = 7), which was not correlated with viability loss (p > 0.05). In parallel, all Aβ concentrations elicited a [Ca] rise but a significant difference was only observed at 20 μM (p < 0.05; n = 7) and a tendency was obtained with 10 μM (p = 0.053; n = 7). In conclusion, Aβ peptide oligomers impair sperm function in vitro, although further studies are required to determine the clinical relevance of these findings.

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Amyloid-like substance in mice and human oocytes and embryos

Pimentel

Navarro

Wang

et al. 2019

J Assist Reprod Genet

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Follicular fluid Aβ40 concentrations may be associated with ongoing pregnancy following in vitro fertilization

Abstract: Aβ40 levels in follicle fluid may be associated with ongoing pregnancy and the moderate expression level of Aβ40 is important for oocytes and embryos development.

Cited by 5 publications

References 27 publications

Screening of Chenopodium hybridum L. for Anti-ovarian Cancer Compounds Using UPLC-Q-Exactive MS and Partial Least Squares Analysis

Screening of Chenopodium hybridum L. for Anti-ovarian Cancer Compounds Using UPLC-Q-Exactive MS and Partial Least Squares Analysis

Alzheimer’s disease-related amyloid-β1–42 peptide induces the loss of human sperm function

Amyloid-like substance in mice and human oocytes and embryos

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