Downregulation of miR-193a-5p correlates with lymph node metastasis and poor prognosis in colorectal cancer

Zhang, Peng; Ji, Dengbo; Han, Han; Shi, Yunfei; Du, Changzheng; Gu, Jin

doi:10.3748/wjg.v20.i34.12241

Cited by 86 publications

(102 citation statements)

References 42 publications

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“…Similar findings are the oncogene BIRC5 , found relevant in LUAD here but only previously described in breast58, bladder59, chondrosarcoma60 and sarcoma61. However, its regulating miRNA (miR-145-5p) has been associated to prognosis in gastric62 and kidney63 tumours. Moreover CDC20, described as prognostic in colon64 and breast65, has been identified as relevant in KICH and LUAD in our study.…”

Section: Discussionsupporting

confidence: 86%

Novel miRNA-mRNA interactions conserved in essential cancer pathways

Andrés-León

Cases²,

Alonso

et al. 2017

Sci Rep

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Cancer is a complex disease in which unrestrained cell proliferation results in tumour development. Extensive research into the molecular mechanisms underlying tumorigenesis has led to the characterization of oncogenes and tumour suppressors that are key elements in cancer growth and progression, as well as that of other important elements like microRNAs. These genes and miRNAs appear to be constitutively deregulated in cancer. To identify signatures of miRNA-mRNA interactions potentially conserved in essential cancer pathways, we have conducted an integrative analysis of transcriptomic data, also taking into account methylation and copy number alterations. We analysed 18,605 raw transcriptome samples from The Cancer Genome Atlas covering 15 of the most common types of human tumours. From this global transcriptome study, we recovered known cancer-associated miRNA-targets and importantly, we identified new potential targets from miRNA families, also analysing the phenotypic outcomes of these genes/mRNAs in terms of survival. Further analyses could lead to novel approaches in cancer therapy.

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Section: Discussionsupporting

confidence: 86%

Novel miRNA-mRNA interactions conserved in essential cancer pathways

Andrés-León

Cases²,

Alonso

et al. 2017

Sci Rep

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“…Recently, miRNA-145-5p has been reported to be involved in several cancers such as gastric cancer, 32 breast cancer, 33 renal cell carcinoma, 34 colorectal cancer, 35 and pancreatic cancer. 36 In NSCLC, miR-145-5p has been reported to be downregulated and to exert tumor-suppressor functions.…”

Section: Introductionmentioning

confidence: 99%

Clinical value of miR-145-5p in NSCLC and potential molecular mechanism exploration: A retrospective study based on GEO, qRT-PCR, and TCGA data

et al. 2017

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MicroRNAs have been reported to be involved in various biological processes. Here, we performed a systematic analysis to explore the clinical value and potential molecular mechanism of miR-145-5p in non-small cell lung cancer. First, a meta-analysis was performed with eligible literature, followed by microRNA microarrays in the Gene Expression Omnibus database, to verify the diagnostic and prognostic values of miR-145-5p. A cohort of 125 clinical paired non-small cell lung cancer samples was next used to detect the level of miR-145-5p and to explore the relationship of miR-145-5p with clinicopathological parameters. The Cancer Genome Atlas database was additionally applied to investigate the role of miR-145-5p in non-small cell lung cancer. The potential targets of miR-145-5p were predicted using 12 online prediction databases to explore the prospective molecular mechanism of miR-145-5p in non-small cell lung cancer. The expression of miR-145-5p in non-small cell lung cancer was significantly lower than that in healthy tissues. And miR-145-5p tended to show better diagnostic performance in lung squamous cell carcinoma than in lung adenocarcinoma. Furthermore, the expression of miR-145-5p was closely associated with lymph node metastasis in non-small cell lung cancer. Gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that the target genes were mainly enriched with enzyme-linked receptor protein signaling pathways, SH3 domain binding, cell leading edge, and adherens junction. The protein-protein interaction network showed that eight hub genes (SMAD4, SMAD2, IRS1, FOXO1, ERBB4, NRAS, ACTB, and ACTG1) might be the key target genes of miR-145-5p in non-small cell lung cancer. The information we obtained might offer new perspectives for clinical diagnosis and treatment for non-small cell lung cancer.

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“…19 In osteosarcoma cells, miR-193a-5p overexpression arrests the cell cycle in the G0/G1 phase significantly inhibits tumor cell proliferation and colony formation and suppresses the migration and invasion of osteosarcoma cells through the ATF2/ATF3/ATF4 signaling pathway. For instance, the downregulation of miR-193a-5p is related to lymph node metastasis and poor survival of patients with colorectal cancer.…”

Section: Discussionmentioning

confidence: 99%

“…[7][8][9] Functional studies have confirmed that miRNA participates in various biological processes including cell development, differentiation, and proliferation. [15][16][17][18][19][20] Although miR-193a-5p has been proven to inhibit cell proliferation and colony formation, induce cell-cycle arrest in the G0/G1 phase and suppress tumor cell invasion, the function and the underlying mechanism of miR-193a-5p in GBM remain elusive. 14 According to previous reports, microRNA-193a-5p (miR-193a-5p) is a tumor suppressor which is lowly expressed in multiple cancers including gastric cancer, breast cancer, prostate cancer, non-small cell lung cancer, osteosarcoma, and ovarian cancer.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‐193a‐5p exerts a tumor suppressor role in glioblastoma via modulating NOVA1

Jin

Wang

et al. 2018

J of Cellular Biochemistry

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Background and Objectives Glioblastoma (GBM) is the most common and lethal of intracranial tumors, which is characterized by extensive proliferation and the diffused invasion of tumor cells. MicroRNA‐193a‐5p (miR‐193a‐5p) have been demonstrated previously as a functional suppressor in the development and progression of various cancers. The current study aimed to investigate whether miR‐193a‐5p influences cell proliferation and migration through the mitogen‐activated protein kinase/extracellular signal‐regulated kinase signaling pathway by targeting neuro‐oncological ventral antigen 1 (NOVA1) in glioblastoma. Materials and Methods The miR‐193a‐5p expression was detected by quantitative real‐time polymerase chain reaction assay in GBM tissues and cell lines. Cell Counting Kit‐8 assay, colony formation analysis, wound‐healing, and transwell invasion assays were performed to evaluate cell proliferation, colony formation, migration, and invasion, respectively. Western blot analysis and luciferase reporter gene assay were performed to verify the downstream target gene of miR‐193a‐5p. Results The expression of miR‐193a‐5p was significantly downregulated in GBM tissues and cell lines. Kaplan‐Meier analysis showed that patients with low miR‐193a‐5p expression had a shorter disease‐free survival (P < 0.05). Functionally, miR‐193a‐5p overexpression dramatically suppressed the proliferation, colony formation, migration, and invasion in glioma cells. Bioinformatics prediction and a luciferase assay confirmed that NOVA1 was a direct functional target of miR‐193a‐5p. Moreover, ectopic expression of NOVA1 could partially reverse the inhibitory effects of miR‐193a‐5p on glioma cell proliferation, colony formation, migration, and invasion. NOVA1 overexpression abrogated the inhibitory effect of miR‐193a‐5p on the PTEN/PI3k/AKT pathway. Conclusion Taken together, our findings suggested that miR‐193a‐5p functions as a tumor suppressor in glioma cells by directly targeting NOVA1.

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Downregulation of miR-193a-5p correlates with lymph node metastasis and poor prognosis in colorectal cancer

Abstract: Downregulation of miR-193a-5p correlates with lymph node metastasis and poor survival of CRC. miR-193a-5p may be a useful biomarker for CRC diagnosis, metastasis and prognosis prediction.

Cited by 86 publications

References 42 publications

Novel miRNA-mRNA interactions conserved in essential cancer pathways

Novel miRNA-mRNA interactions conserved in essential cancer pathways

Clinical value of miR-145-5p in NSCLC and potential molecular mechanism exploration: A retrospective study based on GEO, qRT-PCR, and TCGA data

MicroRNA‐193a‐5p exerts a tumor suppressor role in glioblastoma via modulating NOVA1

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