Strategic Approaches for Enhancement of In Vivo Transbuccal Peptide Drug Delivery in Rabbits using Iontophoresis and Chemical Enhancers

Oh, Dong-Ho; Kim, Min-Ju; Jeon, Sang-Ok; Seo, Jeong-Sun; Jeong, Seong‐Hoon; Kang, Jeong-Won; Choi, Young In; Lee, Sang Kil

doi:10.1007/s11095-014-1507-z

Cited by 9 publications

(4 citation statements)

References 47 publications

(88 reference statements)

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“…Furthermore, SGDC acts as a permeation enhancer and enhances the permeability of hydrophilic compounds via paracellular and transcellular pathways across porcine buccal tissue. 24,48 Dihydroxy bile salts such as SGDC were also shown to enhance paracellular transport at concentrations below 10 mM and opened the transcellular route at concentrations of 10 mM or higher, whereas 0.4 mM SGDC did not show enhancement flux. 49 As per this observation, our findings might have followed a similar trend, as seen from increased insulin permeability from the C1-nanocomplex via increasing intracellular transport as C1 had 5.17 mM SGDC content.…”

Section: Ex Vivo Permeability Studiesmentioning

confidence: 99%

Facilitated Buccal Insulin Delivery via Hydrophobic Ion-Pairing Approach: In vitro and ex vivo Evaluation

Bashyal¹,

Seo²,

Keum³

et al. 2021

IJN

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Background:The clinical use of therapeutic peptides has been limited because of their inefficient delivery approaches and, therefore, inadequate delivery to target sites. Buccal administration of therapeutic peptides offers patients a potential alternative to the current invasive routes of administration. Purpose: The aim of the study was to fabricate hydrophobic ion-pairing (HIP)-nanocomplexes (C1 and C2) utilizing anionic bile salts and cationic peptides, and to assess their permeability across TR146 buccal cell layers and porcine buccal tissue. Methods: C1 and C2-nanocomplexes were fabricated using the HIP approach. In addition, their physiochemical and morphological attributes, in vitro and ex vivo permeability properties, and qualitative and quantitative cellular uptake were evaluated and compared. The localization of C1 and C2-nanocomplexes in porcine buccal tissue was determined using confocal laser scanning microscopy. Results: The C1-nanocomplex was the superior nanocarrier and significantly enhanced the transport of insulin across TR146 cell layers and porcine buccal tissue, exhibiting a 3.00-and 51.76-fold increase in permeability coefficient, respectively, when compared with insulin solution (p < 0.01). C1-nanocomplex was more efficient than C2-nanocomplex at facilitating insulin permeability, with a 2.18-and 27.64-fold increase across TR146 cell layers and porcine buccal tissue, respectively. The C1-nanocomplex demonstrated immense uptake and localization of insulin in TR146 cells and porcine buccal tissue, as evidenced by a highly intense fluorescence in TR146 cells, and a great shift of fluorescence intensity towards the inner region of buccal tissue over time. The increase in fluorescence intensity was observed in the order of C1 > C2 > insulin solution. Conclusion:In this study, we highlighted the efficacy of potential nanocarriers in addressing the daunting issues associated with the invasive administration of insulin and indicated a promising strategy for the buccal administration and delivery of this life-saving peptide hormone.

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Section: Ex Vivo Permeability Studiesmentioning

confidence: 99%

Facilitated Buccal Insulin Delivery via Hydrophobic Ion-Pairing Approach: In vitro and ex vivo Evaluation

Bashyal¹,

Seo²,

Keum³

et al. 2021

IJN

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“…Oh et al showed the combined effects of iontophoresis and enhancer sodium deoxyglycocholate but not N -acetyl- l -cysteine for buccal salmon calcitonin delivery in vitro [ 77 ]. In addition, they demonstrated the combination effects of iontophoresis and chemical enhancers on buccal delivery of salmon calcitonin in rabbits in vivo [ 78 ]. The animal study also showed a decrease in buccal thickness and minor tissue damage that recovered within 24 h after the removal of the iontophoresis electrodes.…”

Section: Iontophoresis On Oral Mucosamentioning

confidence: 99%

Iontophoretic Drug Delivery in the Oral Cavity

Wanasathop

2018

Pharmaceutics

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Iontophoresis is a noninvasive method to enhance systemic and local drug delivery by the application of an electric field. For systemic drug delivery in the oral cavity, iontophoresis was studied primarily for transbuccal delivery. Significant enhancement of drug delivery was observed in buccal iontophoresis compared to passive transport for different drugs. For local drug delivery in the oral cavity, iontophoresis could enhance drug penetration into the enamel, dentin, and other oral tissues for the treatment of oral diseases. Iontophoresis was evaluated in dentistry such as to produce local anesthesia and treat tooth decalcification and hypersensitivity, but this technology has not been fully utilized. The most common drugs in these evaluations were fluoride and lidocaine. In general, there is limited knowledge of the mechanisms of iontophoresis in the oral tissues. In vivo animal and human studies have suggested that iontophoresis is safe in the oral cavity under the conditions investigated. The present review covers the topics of iontophoretic drug delivery in the oral cavity for both systemic and local treatments. The anatomy and diseases in the oral cavity for iontophoretic drug delivery are also briefly reviewed, and the challenges for this drug delivery method are discussed.

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“…This approach is gaining research attention in its ability to control both allergenic and some autoimmune diseases such as experimental autoimmune encephalomyelitis (EAE), arthritis, allergic rhinitis, and asthmas, in clinical studies [101,103,105]. Further, novel peptide administration strategies such as transdermal [106], intranasal [107,108], transbuccal [109,110] and even oral [111,112] routes are also gaining attention. The recognition of alternative ways of administration could enhance the significance of peptide drugs in different disease treatments beyond conventional treatment strategies [7].…”

Section: Administrative Route and Delivery Challengesmentioning

confidence: 99%

Protein and Peptide Biopharmaceuticals: An Overview

Agyei¹,

Ahmed²,

Akram³

et al. 2017

PPL

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Bioactive proteins and peptides are recognised as novel therapeutic molecules with varying biological properties for potential medical applications. Development of protein and peptidebased therapeutic products for human use is growing steadily as they continue to receive an increasing rate of approval by the United States Food and Drugs Administration (US FDA). In this short review, we describe the current status and methodologies involved in the synthesis of protein and peptide biopharmaceuticals with an emphasis on the drivers and restrains to their exploitation in the therapeutic products sector.

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Strategic Approaches for Enhancement of In Vivo Transbuccal Peptide Drug Delivery in Rabbits using Iontophoresis and Chemical Enhancers

Abstract: Iontophoresis and combination with SDGC were found to be safe and potential strategies for transbuccal peptide delivery in vivo.

Cited by 9 publications

References 47 publications

Facilitated Buccal Insulin Delivery via Hydrophobic Ion-Pairing Approach: In vitro and ex vivo Evaluation

Facilitated Buccal Insulin Delivery via Hydrophobic Ion-Pairing Approach: In vitro and ex vivo Evaluation

Iontophoretic Drug Delivery in the Oral Cavity

Protein and Peptide Biopharmaceuticals: An Overview

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