Characterization of two distinct subfamilies of SUN-domain proteins in Arabidopsis and their interactions with the novel KASH-domain protein AtTIK

Graumann, Katja; Vanrobays, Emmanuel; Tutois, Sylvie; Probst, Aline V.; Evans, David; Tatout, Christophe

doi:10.1093/jxb/eru368

Cited by 72 publications

(121 citation statements)

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“…5C; Table S3). Despite KASH and SUN domain proteins being part of the LINC complex (Graumann et al, 2014;Zhou and Meier, 2014), we did not detect any significant change in wifi mutants. This might be due to the potential redundancy with other Arabidopsis KASH domain proteins (Poulet et al, 2016) or alternatively due to a specific function of SUN domain proteins in chromatin organisation.…”

Section: Different Cell Types Show Quantitative Variations In Nuclearcontrasting

confidence: 84%

“…The quintuple wifi mutant (Zhou and Meier, 2014), lacking three KASH proteins WIP1-WIP3 and the two WPP domaininteracting tail-anchored proteins WIT1 and WIT2, was selected to affect the KASH components of the LINC complex as well as some of its interactors located at the outer nuclear membrane. Finally, we combined available sun knockout mutant alleles in the Col-0 background [sun1-1 , sun4-1 and sun5-1 (Graumann et al, 2014)] to obtain double and triple mutants (Fig. S3A).…”

Section: Different Cell Types Show Quantitative Variations In Nuclearmentioning

confidence: 99%

“…S3A). The different mutant combinations of one Cter-SUN1 and two mid-SUN (SUN4 and SUN5) proteins, which include a SUN domain, respectively, at the C-terminal region or in the middle of the protein (Graumann et al, 2014). yield viable plants sun1 sun4 sun5 triple mutants showing increased leaf area compared to the WT (Fig.…”

Section: Different Cell Types Show Quantitative Variations In Nuclearmentioning

confidence: 99%

“…Plants encode a LINC complex consisting of SUN (Sad1 and Unc-84 homology) Graumann et al, 2014) and KASH (Klarsicht, Anc-1 and Syne homology) proteins including WPP domain-interacting proteins (WIPs), SUNinteracting nuclear envelope (SINEs) and TIK (Zhou et al, 2012(Zhou et al, , 2015aGraumann et al, 2014). Furthermore, possible candidates for lamin-like and lamin-binding proteins have been identified and are known, respectively, as CROWDED NUCLEI (CRWN) (Dittmer et al, 2007;Wang et al, 2013) and KAKU4 (Goto et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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The LINC complex contributes to heterochromatin organisation and transcriptional gene silencing in plants

Poulet

Duc

Voisin

et al. 2017

Journal of Cell Science

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The linker of nucleoskeleton and cytoskeleton (LINC) complex is an evolutionarily well-conserved protein bridge connecting the cytoplasmic and nuclear compartments across the nuclear membrane. While recent data support its function in nuclear morphology and meiosis, its involvement in chromatin organisation has not been studied in plants. Here, 3D imaging methods have been used to investigate nuclear morphology and chromatin organisation in interphase nuclei of the model plant Arabidopsis thaliana in which heterochromatin clusters in conspicuous chromatin domains called chromocentres. Chromocentres form a repressive chromatin environment contributing to transcriptional silencing of repeated sequences, a general mechanism needed for genome stability. Quantitative measurements of the 3D position of chromocentres indicate their close proximity to the nuclear periphery but that their position varies with nuclear volume and can be altered in specific mutants affecting the LINC complex. Finally, we propose that the plant LINC complex contributes to proper heterochromatin organisation and positioning at the nuclear periphery, since its alteration is associated with the release of transcriptional silencing as well as decompaction of heterochromatic sequences.

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Section: Different Cell Types Show Quantitative Variations In Nuclearcontrasting

confidence: 84%

Section: Different Cell Types Show Quantitative Variations In Nuclearmentioning

confidence: 99%

Section: Different Cell Types Show Quantitative Variations In Nuclearmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The LINC complex contributes to heterochromatin organisation and transcriptional gene silencing in plants

Poulet

Duc

Voisin

et al. 2017

Journal of Cell Science

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“…Linker of nucleoskeleton and cytoskeleton (LINC) complexes (Box 1; Brkljacic et al, 2009;Graumann, 2014;Graumann et al, 2010Graumann et al, , 2014Tamura et al, 2013;Xu et al, 2007a;Zhao et al, 2008;Zhou et al, 2012Zhou et al, , 2015aZhou et al, , 2015b) bridge the nuclear envelope and are involved in nuclear positioning and migration as well as chromatin organization (reviewed in animals in Starr and Fridolfsson, 2010;Chang et al, 2015;Meier, 2016). Plant LINC complexes have only recently been identified and function in nuclear movement in pollen tubes and male fertility, as well as in nuclear positioning in the leaf epidermis, guard cells, and root hairs ( Fig.…”

Section: The Nuclear Peripherymentioning

confidence: 99%

Dynamic Changes in Plant Nuclear Organization in Response to Environmental and Developmental Signals

et al. 2017

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ORCID ID: 0000-0002-4141-5400 (I.M.).In both plants and animals, the nucleus acts as an organizing center for the changes that occur during an organism's life cycle, whether as part of a developmental program or in response to environmental factors. Here, we cover recent research that explores roles of the nucleus other than gene expression in light response, fertilization, plant-pathogen interactions, bacterial symbiotic events, and hormone signaling. New strides have been made in understanding subnuclear organization, how nuclear organization responds to different environments and developmental stages, and how the cytoplasm-nucleoplasm connections are made that allow these responses. We further highlight new tools that have been developed to study dynamic changes in nuclear organization.The nucleus is compartmentalized by a double membrane called the nuclear envelope (NE), which consists of the outer nuclear membrane (ONM), the inner nuclear membrane (INM), and the embedded nuclear pore complexes (NPCs). The NPC is the primary pathway of macromolecular transport between the nucleus and the cytoplasm and is conserved throughout eukaryotes. The ONM and INM are populated by a variety of membrane-associated proteins, and in particular the inner membrane hosts a specific subset of proteins (Starr and Fridolfsson, 2010;Meier et al., 2017). In addition to functioning in nuclear morphology, chromatin attachment, and likely signal transduction, plant NE-associated proteins are involved in nuclear positioning and movement within the larger cellular context (Griffis et al., 2014). While plant nuclear ultrastructure reveals an inner nuclear membrane-associated meshwork similar to the animal lamina, plant genomes do not encode obvious lamin homologs. Instead, plant-specific long coiled-coil proteins with structural similarity to animal lamins might contribute to this meshwork. Plants also lack centrosomes, and the NE plays a role as the microtubuleorganizing center at the onset of mitosis. NE proteins functioning as calcium channels are involved in perinuclear calcium oscillations, which are an important step in the establishment of plant-symbiont interactions (recently reviewed in Meier et al., 2017).While the nuclear periphery is emerging as an essential organizing platform for the nucleus, intranuclear functional and structural organization might also originate from independent self-assembly processes, with the most conspicuous manifestation of these processes revealed as the nucleolus. New methods are now robustly adapted for plants to reveal greater details of functional nuclear and nucleolar organization. Due to the increasing realization that spatial organization is a crucial part of biological function at the subcellular level, many of these aspects have been recently reviewed

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The Linker of Nucleoskeleton and Cytoskeleton Complex in Higher Plants

Evans

Graumann

2018

Annual Plant Reviews Online

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The linker of nucleoskeleton and cytoskeleton (LINC) complex provides a multi‐functional bridge across the nuclear envelope, connecting nucleoskeleton and cytoskeleton. This article considers the evidence for a LINC complex in higher plants and describes its key components, their functions, and interactions. Evidence is presented that the complex is based on two families of Sad1/UNC84 homology (SUN) domain proteins in the inner nuclear envelope (C‐ter SUNs and mid‐SUNs) and three major groups of Klarsicht/ANC‐1/Syne homology (KASH) domain proteins, WPP‐domain‐interacting proteins (WIPs), SUN‐interacting nuclear envelope proteins (SINEs), and Toll Interleukin Receptor domain KASH protein (TIK). Recent advances in analyzing the function of the complex in establishing nuclear shape and size, nuclear movement, and connecting nucleoskeleton and cytoskeleton are described, together with its role in mitosis and meiosis.

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Characterization of two distinct subfamilies of SUN-domain proteins in Arabidopsis and their interactions with the novel KASH-domain protein AtTIK

Cited by 72 publications

References 37 publications

The LINC complex contributes to heterochromatin organisation and transcriptional gene silencing in plants

The LINC complex contributes to heterochromatin organisation and transcriptional gene silencing in plants

Dynamic Changes in Plant Nuclear Organization in Response to Environmental and Developmental Signals

The Linker of Nucleoskeleton and Cytoskeleton Complex in Higher Plants

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