Characterization of microsatellite loci in <i>Tilia platyphyllos</i> (Malvaceae) and cross‐amplification in related species

Phuekvilai, Prattana; Wolff, Kirsten

doi:10.3732/apps.1200386

Cited by 25 publications

(23 citation statements)

References 9 publications

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“…Approximately 40 μg of fresh leave was used and the step‐by‐step procedure was followed. Genotyping was conducted using 10 polymorphic microsatellites (SSRs) developed by Phuekvilai and Wolff (). One microsatellite, Tc23, which is not included in the final publication by Phuekvilai and Wolff (), was used in this current study.…”

Section: Methodsmentioning

confidence: 99%

Genetic and clonal structures of the tree species Tilia cordata mill. in remnants of ancient forests in Denmark

2019

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The insect-pollinated forest tree Tilia cordata Mill. grows today in small fragmented populations in Denmark and other western European countries but was, in prehistoric times, a dominating species and is considered an indicator species for ancient forest. The species is known to propagate both sexually and vegetatively, forming clonal groups. Few studies have been made on the species' population genetics and on how clonality affects the population structure. The aim of this study is to evaluate the Danish gene pool by estimating genetic diversity and differentiation, as well as through exhaustive sampling describe clonal structures in some of the populations. Genetic analysis was carried out using nine nuclear microsatellite markers in nine populations, of which four were exhaustively or partly exhaustively sampled. The markers showed a high degree of genetic diversity but low differentiation between populations, with no geographic-related structure. Clonal structures were found in eight out of the nine populations. In the exhaustively sampled populations, recruitment strategies included both sexual and clonal reproduction with indications that clonality may be enhanced by management and other disturbances.

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Section: Methodsmentioning

confidence: 99%

Genetic and clonal structures of the tree species Tilia cordata mill. in remnants of ancient forests in Denmark

2019

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“…Nuclear DNA was extracted from the sampled leaves with the QIAGEN ® DNeasy 96 Plant Kit (Germany), using approximately 40 mg of leaf material and following the manufactures instructions. The samples were genotyped for nine nuclear microsatellites developed by Phuekvilai and Wolff (). Tc23 was not described in Phuekvilai and Wolff (), but included in Hansen, Thomsen, and Rasmussen ().…”

Section: Methodsmentioning

confidence: 99%

Section: Molecular Analysis Based On Ssr Markersmentioning

confidence: 99%

“…The samples were genotyped for nine nuclear microsatellites developed by Phuekvilai and Wolff (2013). Tc23 was not described in Phuekvilai and Wolff (2013), but included in Hansen, Thomsen, and Rasmussen (2014). The microsatellites were amplified in three different multiplex primer mixes using Qiagen multiplex PCR kit (Germany) and following the PCR protocol optimized by Phuekvilai and Wolff (2013).…”

Section: Molecular Analysis Based On Ssr Markersmentioning

confidence: 99%

“…Tc23 was not described in Phuekvilai and Wolff (2013), but included in Hansen, Thomsen, and Rasmussen (2014). The microsatellites were amplified in three different multiplex primer mixes using Qiagen multiplex PCR kit (Germany) and following the PCR protocol optimized by Phuekvilai and Wolff (2013). The PCR products were visualized by capillary electrophoresis on an ABI 3130xl sequencer (Applied Biosystems), and size standard GZ500LIZ was applied for reference of fragment size and scored using GeneMapper v.4.0 (Applied Biosystems).…”

Section: Molecular Analysis Based On Ssr Markersmentioning

confidence: 99%

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Local adaptation through genetic differentiation in highly fragmented Tilia cordata populations

Lobo

Hansen

Erichsen

et al. 2018

Ecology and Evolution

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We assessed the level of geographic differentiation of Tilia cordata in Denmark based on tests of 91 trees selected from 12 isolated populations. We used quantitative analysis of spring phenology and population genetic analysis based on SSR markers to infer the likely historical genetic processes within and among populations. High genetic variation within and among populations was observed in spring phenology, which correlated with spring temperatures at the origin of the tested T. cordata trees. The population genetic analysis revealed significant differentiation among the populations, but with no clear sign of isolation by distance. We infer the findings as indications of ongoing fine scale selection in favor of local growth conditions made possible by limited gene flow among the small and fragmented populations. This hypothesis fits well with reports of limited fruiting in the investigated Danish T. cordata populations, while the species is known for its ability to propagate vegetatively by root suckers. Our results suggest that both divergent selection and genetic drift may have played important roles in forming the genetic patterns of T. cordata at its northern distribution limit. However, we also speculate that epigenetic mechanism arising from the original population environment could have created similar patterns in regulating the spring phenology.

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Genetic signature of the natural gene pool of Tilia cordata Mill. in Lithuania: Compound evolutionary and anthropogenic effects

Danusevičius

Kembrytė

Buchovska

et al. 2021

Ecology and Evolution

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Tilia cordata Mill. is a valuable tree species enriching the ecological values of the coniferous‐dominated boreal forests in Europe. Following the historical decline, spreading of Tilia sp. is challenged by the elevated inbreeding and habitat fragmentation. We studied the geographical distribution of genetic diversity of Tilia cordata populations in Lithuania. We used 14 genomic microsatellite markers to genotype 543 individuals from 23 wild‐growing populations. We found that Tilia cordata retained high levels of genetic diversity (population F IS = 0–0.15, H o = 0.53–0.69, H e = 0.56–0.75). AMOVA, Bayesian clustering, and Monmonier's barrier detection indicate weak but significant differentiation among the populations ( F ST = 0.037***) into geographically interpretable clusters of (a) western Lithuania with high genetic heterogeneity but low genetic diversity, bottleneck effects, (b) relatively higher genetic diversity of Tilia cordata on rich and most soils of midland lowland, and (c) the most differentiated populations on poor soils of the coolest northeastern highland possessing the highest rare allele frequency but elevated inbreeding and bottleneck effects. Weak genetic differentiation among the Tilia cordata populations in Lithuania implies common ancestry, absence of strong adaptive gradients, and effective genetic exchange possible mediated via the riparian networks. A hypothesis on riparian networks as gene flow mediators in Tilia cordata was raised based on results of this study.

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Characterization of microsatellite loci in Tilia platyphyllos (Malvaceae) and cross‐amplification in related species

Cited by 25 publications

References 9 publications

Genetic and clonal structures of the tree species Tilia cordata mill. in remnants of ancient forests in Denmark

Genetic and clonal structures of the tree species Tilia cordata mill. in remnants of ancient forests in Denmark

Local adaptation through genetic differentiation in highly fragmented Tilia cordata populations

Genetic signature of the natural gene pool of Tilia cordata Mill. in Lithuania: Compound evolutionary and anthropogenic effects

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