Modulation of dopaminergic neurotransmission induced by sublethal Doses of the organophosphate trichlorfon in cockroaches

̈rmer, Graziele Daiane Stu; Freitas, Thiago Carrazoni de; Heberle, Marines de Avila; Assis, Dênis Reis de; Vinadé, Lúcia; Pereira, Antônio Batista; Franco, Jeferson Luís; Belo, Cháriston André Dal

doi:10.1016/j.ecoenv.2014.08.006

Cited by 23 publications

(5 citation statements)

References 62 publications

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“…In addition, it is essential for melanisation and sclerotisation of the exoskeleton in insects. Functions of dopamine relevant to the present study are its regulation of locomotion and grooming ( Banks and Adams, 2012 ; Daiane Stürmer et al, 2014 ). The action of dopamine is mediated by the rhodopsin-like family of GPCRs ( Bockaert and Pin, 1999 ).…”

Section: Discussionmentioning

confidence: 99%

Parasitoid wasp venom manipulates host innate behavior via subtype-specific dopamine receptor activation

Nordio

Kaiser

Adams

et al. 2022

Journal of Experimental Biology

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The subjugation strategy employed by the jewel wasp is unique in that it manipulates the behavior of its host, the American cockroach, rather than inducing outright paralysis. Upon envenomation directly into the central complex (CX), a command center in the brain for motor behavior, the stung cockroach initially engages in intense grooming behavior, then falls into a lethargic sleep-like state referred to as hypokinesia. Behavioral changes evoked by the sting are due at least in part to the presence of the neurotransmitter dopamine in the venom. In insects, dopamine receptors are classified as two families, the D1-like and the D2-like receptors. However, specific roles played by dopamine receptor subtypes in venom-induced behavioral manipulation by the jewel wasp remain largely unknown. In the present study, we used a pharmacological approach to investigate roles of D1-like and D2-like receptors in behaviors exhibited by stung cockroaches, focusing on grooming. Specifically, we assessed behavioral outcomes of focal CX injections of dopamine receptor agonists and antagonists. Both specific and non-specific compounds were used. Our results strongly implicate D1-like dopamine receptors in venom-induced grooming. Regarding induction of hypokinesia, our findings demonstrate that dopamine signaling is necessary for induction of long-lasting hypokinesia caused by brain envenomation.

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Section: Discussionmentioning

confidence: 99%

Parasitoid wasp venom manipulates host innate behavior via subtype-specific dopamine receptor activation

Nordio

Kaiser

Adams

et al. 2022

Journal of Experimental Biology

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“…At this point, we cannot rule out about the precise molecular target of RSPS upon sensorial afferents. However, the octopaminergic neurotransmission is a candidate, since it was shown to be involved in the modulation of N. cinerea behavior by other toad toxic secretion and OPs [ 25 , 27 ]. In addition, octopamine is a central modulator of insect sensorial responses and positively modulates stretch receptors in Locusta migratoria [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

The Insecticidal Activity of Rhinella schneideri (Werner, 1894) Paratoid Secretion in Nauphoeta cinerea Cocroaches

Leal

Karnopp

Barreto

et al. 2020

Toxins

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Rhinella schneideri is a common toad found in South America, whose paratoid toxic secretion has never been explored as an insecticide. In order to evaluate its insecticidal potential, Nauphoeta cinerea cockroaches were used as an experimental model in biochemical, physiological and behavioral procedures. Lethality assays with Rhinella schneideri paratoid secretion (RSPS) determined the LD50 value after 24 h (58.07µg/g) and 48 h exposure (44.07 µg/g) (R2 = 0.882 and 0.954, respectively). Acetylcholinesterase activity (AChE) after RSPS at its highest dose promoted an enzyme inhibition of 40%, a similar effect observed with neostigmine administration (p < 0.001, n= 5). Insect locomotion recordings revealed that RSPS decreased the distance traveled by up to 37% with a concomitant 85% increase in immobile episodes (p < 0.001, n = 36). RSPS added to in vivo cockroach semi-isolated heart preparation promoted an irreversible and dose dependent decrease in heart rate, showing a complete failure after 30 min recording (p < 0.001, n ≥ 6). In addition, RSPS into nerve-muscle preparations induced a dose-dependent neuromuscular blockade, reaching a total blockage at 70 min at the highest dose applied (p < 0.001, n ≥ 6). The effect of RSPS on spontaneous sensorial action potentials was characterized by an increase in the number of spikes 61% (p < 0.01). Meanwhile, there was 42% decrease in the mean area of those potentials (p < 0.05, n ≥ 6). The results obtained here highlight the potential insecticidal relevance of RSPS and its potential biotechnological application.

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“…Unless otherwise stated, all reagents were purchased from Sigma-Aldrich, Merck, Roche, Life Technologies or BioRad. Test-solutions were prepared daily by dilution in insect saline [50], immediately before use. The insect saline is a carbonate-buffered solution freshly prepared with the following composition in mM: NaCl, 214; KCl, 3.1; CaCl 2 , 9; sucrose, 50; HEPES buffer, 5 and pH 7.2.…”

Section: Methodsmentioning

confidence: 99%

“…The in vitro activity of AChE was evaluated according to Stürmer et al [50]. Six animals were treated with different doses of HPC (100, 200, 400 and 800µg/g body weight).…”

Section: Methodsmentioning

confidence: 99%

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Entomotoxic Activity of Prasiola crispa (Antarctic Algae) in Nauphoeta cinerea Cockroaches: Identification of Main Steroidal Compounds

et al. 2019

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Prasiola crispa is a macroscopic green algae found in abundance in Antarctica ice free areas. Prasiola crispan-hexaneextract (HPC) induced insecticidal activity in Nauphoeta cinerea cockroaches after 24 h of exposure. The chemical analysis of HPC revealed the presence of the followingphytosterols: β-sitosterol, campesterol and stigmasterol. The incubation of cockroach semi-isolated heart preparations with HPC caused a significant negative chronotropic activity in the heartbeats. HPC affected the insect neuromuscular function by inducing a complete inhibition of the cockroach leg-muscle twitch tension. When the isolated phytosterols were injected at in vivo cockroach neuromuscular preparations, there was a progressive inhibition of muscle twitches on the following order of potency: β-sitosterol > campesterol > stigmasterol. HPC also provoked significant behavioral alterations, characterized by the increase or decrease of cockroach grooming activity, depending on the dose assayed. Altogether, the results presented here corroborate the insecticide potential of Prasiola crispa Antarctic algae. They also revealed the presence of phytosterols and the involvement of these steroidal compounds in the entomotoxic activity of the algae, potentially by modulating octopaminergic-cholinergic pathways. Further phytochemical-combined bioguided analysis of the HPC will unveil novel bioactive compounds that might be an accessory to the insecticide activity of the algae.

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Modulation of dopaminergic neurotransmission induced by sublethal Doses of the organophosphate trichlorfon in cockroaches

Cited by 23 publications

References 62 publications

Parasitoid wasp venom manipulates host innate behavior via subtype-specific dopamine receptor activation

Parasitoid wasp venom manipulates host innate behavior via subtype-specific dopamine receptor activation

The Insecticidal Activity of Rhinella schneideri (Werner, 1894) Paratoid Secretion in Nauphoeta cinerea Cocroaches

Entomotoxic Activity of Prasiola crispa (Antarctic Algae) in Nauphoeta cinerea Cockroaches: Identification of Main Steroidal Compounds

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