Characterization and molecular mapping of stripe rust resistance gene Yr61 in winter wheat cultivar Pindong 34

Abstract

We report a new stripe rust resistance gene on chromosome 7AS in wheat and molecular markers useful for transferring it to other wheat genotypes. Several new races of the stripe rust pathogen have established throughout the wheat growing regions of China in recent years. These new races are virulent to most of the designated seedling resistance genes limiting the resistance sources. It is necessary to identify new genes for diversification and for pyramiding different resistance genes in order to achieve more durable resistance. We report here the identification of a new resistance gene, designated as Yr61, in Chinese wheat cultivar Pindong 34. A mapping population of 208 F2 plants and 128 derived F2:3 lines in a cross between Mingxian 169 and Pindong 34 was evaluated for seedling stripe rust response. A genetic map consisting of eight resistance gene analog polymorphism (RGAP), two sequence-tagged site (STS) and four simple sequence repeat (SSR) markers was constructed. Yr61 was located on the short arm of chromosome 7A and flanked by RGAP markers Xwgp5467 and Xwgp5765 about 1.9 and 3.9 cM in distance, which were successfully converted into STS markers STS5467 and STS5765b, respectively. The flanking STS markers could be used for marker-assisted selection of Yr61 in breeding programs.

Loci associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in a core collection of spring wheat (Triticum aestivum)

“…The identity or similarity of the genomic region tagged by IWA7306 and IWA1845 with previously mapped QTL needs to be further investigated. Although Yr61 [69] and Yrxy1 [70] have already been mapped on the short arm of chromosome 7A, both IWA7306 and IWA1845 were mapped far from Yr61 and Yrxy1 , and likely represent different resistance loci.…”

Genome-wide DArT and SNP scan for QTL associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in elite ICARDA wheat (Triticum aestivum L.) germplasm

“…To date, about 67 stripe rust resistance genes (Yr1-Yr67) plus 42 temporarily designated genes have been catalogued in cultivated wheat and its wild relatives (McIntosh et al 2013;Macceferri et al 2015;Cheng et al 2014;Zhou et al 2014). To date, about 67 stripe rust resistance genes (Yr1-Yr67) plus 42 temporarily designated genes have been catalogued in cultivated wheat and its wild relatives (McIntosh et al 2013;Macceferri et al 2015;Cheng et al 2014;Zhou et al 2014).…”

Genome Wide Association Study of Resistance to PstS2 and Warrior Races of Stripe (Yellow) Rust in Bread Wheat Landraces

“…is reported as an all stage resistant gene (Zhou et al, 2014) and the presence of both seedling and adult plant resistance QTL in the genomic region reveals that the identified QTL in this study are related to Yr61.…”