A novel bioassay for evaluating the efficacy of biocides to inhibit settling and early establishment of marine biofilms

Arrhenius, Åsa; Backhaus, Thomas; Hilvarsson, Annelie; Wendt, Ida; Zgrundo, Aleksandra; Blanck, Hans

doi:10.1016/j.marpolbul.2014.07.011

Cited by 22 publications

(18 citation statements)

References 46 publications

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“…Recently, Arrhenius et al . () developed a new assay, based on the capacity of organisms to colonise new surfaces after chronic exposure to biocides. The use of such endpoints is relevant because they not only provide information on the ability of communities to recover following disturbances, but also are not connected to a specific mode of action of a toxicant or to a given biological component within the community.…”

Section: Endpoints To Measure Community Tolerance In the Fieldmentioning

confidence: 99%

“…Thus, one of the challenges in applying PICT for assessing mixtures is to develop an endpoint for chemicals independent of their mode of action and reflecting the response of all the different biological components of the exposed community. Recently, Arrhenius et al (2014) developed a new assay, based on the capacity of organisms to colonise new surfaces after chronic exposure to biocides. The use of such endpoints is relevant because they not only provide information on the ability of communities to recover following disturbances, but also are not connected to a specific mode of action of a toxicant or to a given biological component within the community.…”

Section: Endpoints To Measure Community Tolerance In the Fieldmentioning

confidence: 99%

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Pollution‐induced community tolerance (PICT): towards an ecologically relevant risk assessment of chemicals in aquatic systems

et al. 2015

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SUMMARY1. A major challenge in environmental risk assessment of pollutants is establishing a causal relationship between field exposure and community effects that integrates both structural and functional complexity within ecosystems. 2. Pollution-induced community tolerance (PICT) is a concept that evaluates whether pollutants have exerted a selection pressure on natural communities. PICT detects whether a pollutant has eliminated sensitive species from a community and thereby increased its tolerance. PICT has the potential to link assessments of the ecological and chemical status of ecosystems by providing causal analysis for effect-based monitoring of impacted field sites. 3. Using PICT measurements and microbial community endpoints in environmental assessment schemes could give more ecological relevance to the tools that are now used in environmental risk assessment. Here, we propose practical guidance and a list of research issues that should be further considered to apply the PICT concept in the field.

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Section: Endpoints To Measure Community Tolerance In the Fieldmentioning

confidence: 99%

Section: Endpoints To Measure Community Tolerance In the Fieldmentioning

confidence: 99%

Pollution‐induced community tolerance (PICT): towards an ecologically relevant risk assessment of chemicals in aquatic systems

et al. 2015

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“…PCC7942 is stimulated by Irgarol 1051, with altered physiological composition of both soluble proteins and polysaccharide content in addition to the induction of oxidative stress [ 91 ]. As compensation for inhibited photosynthesis, Irgarol 1051 exerts a selection bias for tolerant algal species in the marine periphyton communities [ 92 ]. Further, exposure to Italic 1051 increases the abnormality and DNA strand breaks in the larvae of Pacific oyster ( C. gigas ) [ 88 ].…”

Section: Antifouling Compounds With Proposed General Targetsmentioning

confidence: 99%

“…Similarly, inhibited photosynthesis and carbon incorporation are detected for SeaNine 211 in the sugar kelp S. latissima [ 89 ]. SeaNine 211 exposure induces oxidative stress by dramatically depleting the intracellular pools of glutathione [ 92 ], or changing antioxidant enzyme activity [ 94 ]. A 28-day exposure to 1.0 µg/L of SeaNine 211 also increases apoptosis in testicular germ cells of the marine teleost mummichog Fundulus heteroclitus , probably through a caspase-dependent pathway [ 95 ].…”

Section: Antifouling Compounds With Proposed General Targetsmentioning

confidence: 99%

Review on Molecular Mechanisms of Antifouling Compounds: An Update since 2012

Chen

Qian

2017

Marine Drugs

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Better understanding of the mechanisms of antifouling compounds is recognized to be of high value in establishing sensitive biomarkers, allowing the targeted optimization of antifouling compounds and guaranteeing environmental safety. Despite vigorous efforts to find new antifouling compounds, information about the mechanisms of antifouling is still scarce. This review summarizes the progress into understanding the molecular mechanisms underlying antifouling activity since 2012. Non-toxic mechanisms aimed at specific targets, including inhibitors of transmembrane transport, quorum sensing inhibitors, neurotransmission blockers, adhesive production/release inhibitors and enzyme/protein inhibitors, are put forward for natural antifouling products or shelf-stable chemicals. Several molecular targets show good potential for use as biomarkers in future mechanistic screening, such as acetylcholine esterase for neurotransmission, phenoloxidase/tyrosinase for the formation of adhesive plaques, N-acyl homoserine lactone for quorum sensing and intracellular Ca2+ levels as second messenger. The studies on overall responses to challenges by antifoulants can be categorized as general targets, including protein expression/metabolic activity regulators, oxidative stress inducers, neurotransmission blockers, surface modifiers, biofilm inhibitors, adhesive production/release inhibitors and toxic killing. Given the current situation and the knowledge gaps regarding the development of alternative antifoulants, a basic workflow is proposed that covers the indispensable steps, including preliminary mechanism- or bioassay-guided screening, evaluation of environmental risks, field antifouling performance, clarification of antifouling mechanisms and the establishment of sensitive biomarkers, which are combined to construct a positive feedback loop.

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“…They are widely distributed and play significant roles in the primary productivity, energy flow, and nutrients cycling in aquatic ecosystems (Azim et al 2005;Battin et al 2003). As a consequence, periphyton biofilms are frequently used for the monitoring of water quality (Sabater et al 2007), bioremediation of contaminated surface water (Wu et al 2014), and toxicity assessment of contaminants (Arrhenius et al 2014;Quinlan et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Effect of butyl paraben on the development and microbial composition of periphyton

et al. 2015

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Parabens are extensively used as preservatives and bactericides in personal care and other consumer products, and are commonly found in wastewater and surface water as contaminants. However, few data are currently available on the ecotoxicity of parabens. Periphyton biofilm, a widely distributed microbial aggregate of ecological importance in aquatic environment, is frequently used for water quality monitoring, ecological restoration, and toxicity assessment. In this work, the effects of butyl paraben on the development and microbial composition of periphyton biofilm was studied in a laboratory experiment for 32 days using flow through channels. No effect was observed at the environmental relevant concentration level (0.5 lg L -1 ) during the experiment. At the highest tested concentration level (5000 lg L -1 ), following effects were noted: (1) inhibition on algae growth at the end of the experiment as indicated by the chlorophyll a and total biovolume; (2) inhibition of photosynthetic efficiency on day 24 as suggested by the maximal Photosystem II quantum yield (Fv/Fm); (3) decrease of the algal diversity on day 24 and 32 as reflected by the Pielou and Shannon-Weiner indices. Bacteria were less sensitive than algae in the periphyton biofilm, which showed no difference at all tested concentration levels as illustrated by the Biolog EcoPlates TM analysis. Therefore, we conclude that environmental residues of butyl paraben have a very low risk to periphyton in aquatic ecosystems.

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A novel bioassay for evaluating the efficacy of biocides to inhibit settling and early establishment of marine biofilms

Cited by 22 publications

References 46 publications

Pollution‐induced community tolerance (PICT): towards an ecologically relevant risk assessment of chemicals in aquatic systems

Pollution‐induced community tolerance (PICT): towards an ecologically relevant risk assessment of chemicals in aquatic systems

Review on Molecular Mechanisms of Antifouling Compounds: An Update since 2012

Effect of butyl paraben on the development and microbial composition of periphyton

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