Wild Saccharomyces cerevisiae strains display biofilm-like morphology in contact with polyphenols from grapes and wine

Sidari, Rossana; Caridi, Andrea

doi:10.1016/j.ijfoodmicro.2014.08.012

Cited by 16 publications

(8 citation statements)

References 44 publications

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“…Moreover, it may be useful to form associations with other microorganisms. Like S. cerevisiae wild type strains isolated from must and grapes (Sidari et al, 2014) the K. marxianus strains analyzed in this work, were able to form diverse mat structures in terms of size and architecture (Figure 2). The genome differences may explain these variances as suggested by Borneman et al (2008, 2011, 2016) for wine strains.…”

Section: Discussionmentioning

confidence: 90%

Cell Wall Surface Properties of Kluyveromyces marxianus Strains From Dairy-Products

et al. 2019

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Thirty-three Kluyveromyces marxianus strains were tested for the ability to form biofilm and mat structures in YPD and whey and for cell surface hydrophobicity. To identify genes potentially involved in adhesion properties, a RT-qPCR analysis was performed. Eight strains were able to adhere on polystyrene plates in both media and formed a mature mat structure. These strains showed a different level of hydrophobicity ranging from 55 to 66% in YPD and from 69 to 81% in whey. Four K. marxianus orthologs genes (FLO11, STE12, TPK3, and WSC4), known from studies in other yeast to be involved in biofilm formation, have been studied. FLO11 and STE12 showed the highest fold changes in all conditions tested and especially in whey: 15.05 and 11.21, respectively. TPK3 was upregulated only in a strain, and WSC4 in 3 strains. In YPD, fold changes were lower than in whey with STE12 and FLO11 genes showing the highest fold changes. In mat structures FLO11 and STE12 fold changes ranged from 3.6–1.3 to 2–1.17, respectively. Further studies are necessary to better understand the role of these genes in K. marxianus adhesion ability.

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Section: Discussionmentioning

confidence: 90%

Cell Wall Surface Properties of Kluyveromyces marxianus Strains From Dairy-Products

et al. 2019

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“…Although filamentous growth is common among “wild” S. cerevisiae strains (Carstens et al, 1998; Sidari et al, 2014), the triggers of filamentous growth have not been extensively characterized in other backgrounds. By examining a collection of wine yeast, we show that most wine strains undergo filamentous growth.…”

Section: Discussionmentioning

confidence: 99%

Role of Mitochondrial Retrograde Pathway in Regulating Ethanol-Inducible Filamentous Growth in Yeast

et al. 2017

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In yeast, ethanol is produced as a by-product of fermentation through glycolysis. Ethanol also stimulates a developmental foraging response called filamentous growth and is thought to act as a quorum-sensing molecule. Ethanol-inducible filamentous growth was examined in a small collection of wine/European strains, which validated ethanol as an inducer of filamentous growth. Wine strains also showed variability in their filamentation responses, which illustrates the striking phenotypic differences that can occur among individuals. Ethanol-inducible filamentous growth in Σ1278b strains was independent of several of the major filamentation regulatory pathways [including fMAPK, RAS-cAMP, Snf1, Rpd3(L), and Rim101] but required the mitochondrial retrograde (RTG) pathway, an inter-organellar signaling pathway that controls the nuclear response to defects in mitochondrial function. The RTG pathway regulated ethanol-dependent filamentous growth by maintaining flux through the TCA cycle. The ethanol-dependent invasive growth response required the polarisome and transcriptional induction of the cell adhesion molecule Flo11p. Our results validate established stimuli that trigger filamentous growth and show how stimuli can trigger highly specific responses among individuals. Our results also connect an inter-organellar pathway to a quorum sensing response in fungi.

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“…Biofilm formed by S. cerevisiae in wine fermentation is an adaptive mechanism for continued growth on non-fermentable ethanol (Zara et al, 2010). And S. cerevisiae strains also display biofilm-like morphology as an adaptive mechanism for polyphenols from grapes and wine (Sidari et al, 2014). Thus, mixed biofilm could be a survival strategy not only for species in a fermentation starter but also for other unculturable species in complex microbial communities.…”

Section: Discussionmentioning

confidence: 99%

Formation of a Mixed-Species Biofilm Is a Survival Strategy for Unculturable Lactic Acid Bacteria and Saccharomyces cerevisiae in Daqu, a Chinese Traditional Fermentation Starter

et al. 2020

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Wild Saccharomyces cerevisiae strains display biofilm-like morphology in contact with polyphenols from grapes and wine

Cited by 16 publications

References 44 publications

Cell Wall Surface Properties of Kluyveromyces marxianus Strains From Dairy-Products

Cell Wall Surface Properties of Kluyveromyces marxianus Strains From Dairy-Products

Role of Mitochondrial Retrograde Pathway in Regulating Ethanol-Inducible Filamentous Growth in Yeast

Formation of a Mixed-Species Biofilm Is a Survival Strategy for Unculturable Lactic Acid Bacteria and Saccharomyces cerevisiae in Daqu, a Chinese Traditional Fermentation Starter

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