Ectopic expression of FaesAP3, a Fagopyrum esculentum (Polygonaceae) AP3 orthologous gene rescues stamen development in an Arabidopsis ap3 mutant

Fang, Zhengwu; Qi, Ruili; Li, Xiaofang; Liu, Zhixiong

doi:10.1016/j.gene.2014.08.029

Cited by 19 publications

(17 citation statements)

References 26 publications

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“…Earlier, we suggest that Bclass genes are not involved in petaloidy in buckwheat based on the phenotype of a buckwheat homeotic mutant (Logacheva et al, 2008); this hypothesis now gains support from the obtained expression profiles. Studies on the complementation of Arabidopsis ap3 and pi mutants via the overexpression of buckwheat AP3 and PI genes also show limited conservation of function (Fang et al, 2015;Fang et al, 2014). An alternative programme of petal development, which is not I n r e v i e w dependent on B-class genes, was also found in a family of the core Caryophyllales, Aizoaceae (Brockington et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

High-resolution transcriptome atlas and improved genome assembly of common buckwheat,Fagopyrum esculentum

Penin

Kasianov

Klepikova

et al. 2020

Preprint

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SummaryCommon buckwheat (Fagopyrum esculentum) is an important non-cereal grain crop and a prospective component of functional food. Despite this, the genomic resources for this species and for the whole family Polygonaceae, to which it belongs, are scarce. Here, we report the assembly of the buckwheat genome using long-read technology and a high-resolution expression atlas including 46 organs and developmental stages. We found that the buckwheat genome has an extremely high content of transposable elements, including several classes of recently (0.5-1 Mya) multiplied TEs (“transposon burst”) and gradually accumulated TEs. The difference in TE content is a major factor contributing to the 3-fold increase in the genome size of F. esculentum compared with its sister species F. tataricum. Moreover, we detected the differences in TE content between the wild ancestral subspecies F. esculentum ssp. ancestrale and buckwheat cultivars, suggesting that TE activity accompanied buckwheat domestication. Expression profiling allowed us to test a hypothesis about the genetic control of petaloidy in buckwheat. We showed that it is not mediated by B-class gene activity, in contrast to the prediction from the ABC model. Based on a survey of expression profiles and phylogenetic analysis, we identified the MYB family transcription factor gene tr_18111 as a potential candidate for the determination of conical cells in buckwheat petaloid tepals. The information on expression patterns has been integrated into the publicly available database TraVA: http://travadb.org/browse/Species=Fesc/. The improved genome assembly and transcriptomic resources will enable research on buckwheat, including practical applications.

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Section: Resultsmentioning

confidence: 99%

High-resolution transcriptome atlas and improved genome assembly of common buckwheat,Fagopyrum esculentum

Penin

Kasianov

Klepikova

et al. 2020

Preprint

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“…These results showed a broader expression zones than its orthologs in core eudicots [1,7,8], but a similar expression pattern with AP1-/FUL -like genes from eudicots or basal angiosperms [13,14]. Moreover, the PISTILLATA ( PI ) and APETALA3 ( AP3 ) orthologs commonly involving in specifying petal identity in most core eudicots are proved to be only responsible for stamen development in F. esculentum [29,30], which may suggest a different perianth identity program in F. esculentum . Furthermore, ectopic expression of FaesAP1 in Arabidopsis ap1-10 mutant fully complement sepal development in whorl 1 and produced flower with three whorls of floral organs (sepal whorl, stamen whorl and carpel whorl), which completely correspond to floral architecture of F. esculentum with one single whorl of undifferentiated tepals and inner two whorls of reproductive structure (stamen and carpel).…”

Section: Discussionmentioning

confidence: 99%

Ectopic Expression of a Fagopyrum esculentum APETALA1 Ortholog only Rescues Sepal Development in Arabidopsis ap1 Mutant

Liu

Fei

Zhang

et al. 2019

IJMS

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Fagopyrum esculentum (Polygonaceae: Caryophyllales) exhibits an undifferentiated perianth comprising five showy tepals, which does not completely correspond to the perianth differentiated into typical sepals and petals in most core eudicots. In Arabidopsis, the APETALA1 (AP1) gene is involved in specifying sepals and petals development. Here we isolated AP1 ortholog, FaesAP1, and a 2.2kb FaesAP1 promoter (pFaesAP1) from F. esculentum. FaesAP1 expression is mainly detectable in all floral organs and maintains at a high level when tepals elongate rapidly both in pin and thrum flowers. Moreover, the GUS reporter gene driven by pFaesAP1 was activated in flowers where the sepals were intense, but the petals very weak or absent. Additionally, FaesAP1 ectopic expression in Arabidopsis ap1-10 mutant rescues sepal development fully, obviously prompting early flowering, but failing to complement petal development. In this study, evidence was provided that the showy tepals in the F. esculentum are homologs to core eudicots sepals. Furthermore, these findings show a different perianth identity program in Caryophyllales, suggesting that AP1 orthologs involved in petal development may evolve independently across different clades of core eudicots. Our results also suggest that FaesAP1 holds potential for biotechnical engineering to develop early flowering varieties of F. esculentum.

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“…This hypothesis now gains support from the obtained expression profiles. Studies on the complementation of Arabidopsis ap3 and pi mutants via the overexpression of buckwheat AP3 and PI genes also show limited conservation of function (Fang et al, 2014(Fang et al, , 2015. An alternative program of petal development, which is not dependent on B-class genes, was also found in a family of the core Caryophyllales, Aizoaceae (Brockington et al, 2012).…”

Section: The Transcriptome Atlas Provides a Clue Regarding The Originmentioning

confidence: 99%

High-Resolution Transcriptome Atlas and Improved Genome Assembly of Common Buckwheat, Fagopyrum esculentum

et al. 2021

View full text Add to dashboard Cite

Common buckwheat (Fagopyrum esculentum) is an important non-cereal grain crop and a prospective component of functional food. Despite this, the genomic resources for this species and for the whole family Polygonaceae, to which it belongs, are scarce. Here, we report the assembly of the buckwheat genome using long-read technology and a high-resolution expression atlas including 46 organs and developmental stages. We found that the buckwheat genome has an extremely high content of transposable elements, including several classes of recently (0.5–1 Mya) multiplied TEs (“transposon burst”) and gradually accumulated TEs. The difference in TE content is a major factor contributing to the three-fold increase in the genome size of F. esculentum compared with its sister species F. tataricum. Moreover, we detected the differences in TE content between the wild ancestral subspecies F. esculentum ssp. ancestrale and buckwheat cultivars, suggesting that TE activity accompanied buckwheat domestication. Expression profiling allowed us to test a hypothesis about the genetic control of petaloidy of tepals in buckwheat. We showed that it is not mediated by B-class gene activity, in contrast to the prediction from the ABC model. Based on a survey of expression profiles and phylogenetic analysis, we identified the MYB family transcription factor gene tr_18111 as a potential candidate for the determination of conical cells in buckwheat petaloid tepals. The information on expression patterns has been integrated into the publicly available database TraVA: http://travadb.org/browse/Species=Fesc/. The improved genome assembly and transcriptomic resources will enable research on buckwheat, including practical applications.

show abstract

Ectopic expression of FaesAP3, a Fagopyrum esculentum (Polygonaceae) AP3 orthologous gene rescues stamen development in an Arabidopsis ap3 mutant

Cited by 19 publications

References 26 publications

High-resolution transcriptome atlas and improved genome assembly of common buckwheat,Fagopyrum esculentum

High-resolution transcriptome atlas and improved genome assembly of common buckwheat,Fagopyrum esculentum

Ectopic Expression of a Fagopyrum esculentum APETALA1 Ortholog only Rescues Sepal Development in Arabidopsis ap1 Mutant

High-Resolution Transcriptome Atlas and Improved Genome Assembly of Common Buckwheat, Fagopyrum esculentum

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