Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds

Evaristo, Thaiane Cristine; CruzAlves, Flávia Cilene Maciel da; Moroz, Andrei; Mion, Woner; Acorci-Valério, Michele Janegitz; Felisbino, Sérgio Luís; Rossi-Ferreira, Rosana; Júnior, Raul Lopes Ruiz; Deffune, Elenice

doi:10.1590/s0102-86502014000800002

Cited by 15 publications

(11 citation statements)

References 29 publications

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“…Ideally, these scaffolds should be biomechanically compatible with the native trachea, as well as implantable in a feasible manner. 3 Regarding the trachea, the strategies used for decellularization are heterogeneous and there is no a standard protocol. Virtually all protocols that remove cells from the different tissues conforming the tracheal architecture include the use of a non-ionic detergent.…”

Section: Introductionmentioning

confidence: 99%

Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

et al. 2021

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Objective: The aim of this study was to optimize a decellularization protocol in the trachea of Sus scrofa domestica (pig) as well as to study the effects of long-term cryopreservation on the extracellular matrix of decellularized tracheas. Methods: Porcine tracheas were decellularized using Triton X-100, SDC, and SDS alone or in combination. The effect of these detergents on the extracellular matrix characteristics of decellularized porcine tracheas was evaluated at the histological, biomechanical, and biocompatibility level. Morphometric approaches were used to estimate the effect of detergents on the collagen and elastic fibers content as well as on the removal of chondrocytes from decellularized organs. Moreover, the long-term structural, ultrastructural, and biomechanical effect of cryopreservation of decellularized tracheas were also estimated. Results: Two percent SDS was the most effective detergent tested concerning cell removal and preservation of the histological and biomechanical properties of the tracheal wall. However, long-term cryopreservation had no an appreciable effect on the structure, ultrastructure, and biomechanics of decellularized tracheal rings. Conclusion: The results presented here reinforce the use of SDS as a valuable decellularizing agent for porcine tracheas. Furthermore, a cryogenic preservation protocol is described, which has minimal impact on the histological and biomechanical properties of decellularized porcine tracheas.

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Section: Introductionmentioning

confidence: 99%

Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

et al. 2021

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“…Decellularized ECM has also been shown to elicit an anti-inflammatory immune response, which may be related to a reduced risk of rejection [6,7]. Decellularization is accomplished using a variety of techniques, including physical [8], chemical [9], and enzymatic treatment methods [10]. Treatment with aqueous detergents, such as sodium dodecyl sulfate (SDS), is most common [11,12].…”

Section: Introductionmentioning

confidence: 99%

A novel supercritical CO 2 -based decellularization method for maintaining scaffold hydration and mechanical properties

Casali

Handleton

Shazly

et al. 2018

The Journal of Supercritical Fluids

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A B S T R A C TDecellularized tissues are commonly utilized as tissue engineering scaffolds. Decellularization by extended exposure to aqueous detergents can damage the microstructure or deposit cytotoxic residue. Supercritical carbon dioxide (scCO 2 ) has been proposed for decellularization, but reportedly causes dehydration and scaffold embrittlement.Presented herein is a novel decellularization method that preserves matrix hydration state and mechanical properties. Over 97% of the water in porcine aorta is maintained by presaturating scCO 2 with water; however, complete decellularization was not attained by any process utilizing only scCO 2 . Instead, a novel hybrid method is presented that combines a brief (48 h) exposure of tissue to aqueous detergent, followed by washing with scCO 2 (1 h). The hybrid method fully decellularized the tissue, as confirmed by histology and DNA quantification (<0.04 μg DNA/mg tissue). This hybrid treatment was faster than the standard method (2 days compared to 4-7 days), while preserving tissue structure and mechanical properties.

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“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate laryngeal tissue engineering 85‐87,91,92,94,96,97,198‐306 …”

Section: Discussionmentioning

confidence: 99%

Tissue engineering applications in otolaryngology—The state of translation

Niermeyer

Rodman

et al. 2020

Laryngoscope Investig Oto

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While tissue engineering holds significant potential to address current limitations in reconstructive surgery of the head and neck, few constructs have made their way into routine clinical use. In this review, we aim to appraise the state of head and neck tissue engineering over the past five years, with a specific focus on otologic, nasal, craniofacial bone, and laryngotracheal applications. A comprehensive scoping search of the PubMed database was performed and over 2000 article hits were returned with 290 articles included in the final review. These publications have addressed the hallmark characteristics of tissue engineering (cellular source, scaffold, and growth signaling) for head and neck anatomical sites. While there have been promising reports of effective tissue engineered interventions in small groups of human patients, the majority of research remains constrained to in vitro and in vivo studies aimed at furthering the understanding of the biological processes involved in tissue engineering. Further, differences in functional and cosmetic properties of the ear, nose, airway, and craniofacial bone affect the emphasis of investigation at each site. While otolaryngologists currently play a role in tissue engineering translational research, continued multidisciplinary efforts will likely be required to push the state of translation towards tissue‐engineered constructs available for routine clinical use. Level of Evidence NA.

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Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds

Cited by 15 publications

References 29 publications

Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

A novel supercritical CO 2 -based decellularization method for maintaining scaffold hydration and mechanical properties

Tissue engineering applications in otolaryngology—The state of translation

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