Lipid interactions during virus entry and infection

Mazzon, Michela; Mercer, Jason

doi:10.1111/cmi.12340

Cited by 117 publications

(106 citation statements)

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Section: Vaccinia Mature Virions Of the Wr Strain Enter Hela Cells Thmentioning

confidence: 99%

“…However, Laliberte and Moss demonstrated that phospholipids other than PS could also reconstitute MV infectivity (23). To date, there is no evidence that any particular PS receptor is required for vaccinia MV entry (24)(25)(26).Following internalization into cells, the vaccinia virus MVcontaining vesicles are internalized into CD98-positive and phosphatidylinositol 3-phosphate (PI3P)-positive macropinosomes and subsequently fused with Rab5-positive early endosomes (19). However, little is known about the process of sorting the MVcontaining vesicles in cells prior to membrane fusion.…”

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confidence: 99%

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Intracellular Transport of Vaccinia Virus in HeLa Cells Requires WASH-VPEF/FAM21-Retromer Complexes and Recycling Molecules Rab11 and Rab22

Chu¹,

Lee

et al. 2015

J Virol

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Vaccinia virus, the prototype of the Orthopoxvirus genus in the family Poxviridae, infects a wide range of cell lines and animals. Vaccinia mature virus particles of the WR strain reportedly enter HeLa cells through fluid-phase endocytosis. However, the intracellular trafficking process of the vaccinia mature virus between cellular uptake and membrane fusion remains unknown. We used live imaging of single virus particles with a combination of various cellular vesicle markers, to track fluorescent vaccinia mature virus particle movement in cells. Furthermore, we performed functional interference assays to perturb distinct vesicle trafficking processes in order to delineate the specific route undertaken by vaccinia mature virus prior to membrane fusion and virus core uncoating in cells. Our results showed that vaccinia virus traffics to early endosomes, where recycling endosome markers Rab11 and Rab22 are recruited to participate in subsequent virus trafficking prior to virus core uncoating in the cytoplasm. Furthermore, we identified WASH-VPEF/FAM21-retromer complexes that mediate endosome fission and sorting of virus-containing vesicles prior to virus core uncoating in the cytoplasm. IMPORTANCE Vaccinia mature virions of the WR strain enter HeLa cells through fluid phase endocytosis.We previously demonstrated that virus-containing vesicles are internalized into phosphatidylinositol 3-phosphate positive macropinosomes, which are then fused with Rab5-positive early endosomes. However, the subsequent process of sorting the virion-containing vesicles prior to membrane fusion remains unclear. We dissected the intracellular trafficking pathway of vaccinia mature virions in cells up to virus core uncoating in cytoplasm. We show that vaccinia mature virions first travel to early endosomes. Subsequent trafficking events require the important endosome-tethered protein VPEF/FAM21, which recruits WASH and retromer protein complexes to the endosome. There, the complex executes endosomal membrane fission and cargo sorting to the Rab11-positive and Rab22-positive recycling pathway, resulting in membrane fusion and virus core uncoating in the cytoplasm. V accinia virus is the prototype of the Orthopoxvirus genus in the family Poxviridae, which includes the variola virus that causes smallpox diseases. It has a broad host range and infects a wide variety of cell lines and animals. Vaccinia virus produces two forms of infectious virions, mature virus (MV) and extracellular virus (EV), which contain different membrane proteins (1). MV particles are abundant in infected cells and contain ϳ80 viral proteins (2, 3), which contribute to the complex virus entry processes that reportedly vary among different cells and virus strains (4-12).In HeLa cells, vaccinia MV initially attaches to cellular surface component glycosaminoglycans (13-15) and the extracellular matrix protein laminin (16). MV particles then cluster at plasma membrane lipid rafts (17) where the virus further interacts with integrin ␤1 (18) and CD98 receptor molecu...

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Section: Vaccinia Mature Virions Of the Wr Strain Enter Hela Cells Thmentioning

confidence: 99%

mentioning

confidence: 99%

Intracellular Transport of Vaccinia Virus in HeLa Cells Requires WASH-VPEF/FAM21-Retromer Complexes and Recycling Molecules Rab11 and Rab22

Chu¹,

Lee

et al. 2015

J Virol

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“…KHV replication was confirmed by transmission electron microscopy analysis, demonstrating viral particles inside the nucleus of CCB and KoK cells. KoK, but not CCB cells, produced large lipid droplets (data not shown), which might be a sign of accelerated lipid metabolism upon virus replication (Mazzon & Mercer, ). Electron microscopy analysis of ASK and Fr994 revealed replication events, that are typical for members of the orthomyxovirus family, for example, influenza viruses (Nayak, Hui, & Barman, ).…”

Section: Discussionmentioning

confidence: 87%

New cell lines for efficient propagation of koi herpesvirus and infectious salmon anaemia virus

Eckart

Yamaguchi

Franzke

et al. 2018

Journal of Fish Diseases

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The production of piscine viruses, in particular of koi herpesvirus (KHV, CyHV‐3) and infectious salmon anaemia virus (ISAV), is still challenging due to the limited susceptibility of available cell lines to these viruses. A number of cell lines from different fish species were compared to standard diagnostic cell lines for KHV and ISAV regarding their capability to exhibit a cytopathic effect (CPE) and to accumulate virus. Two cell lines, so far undescribed, appeared to be useful for diagnostic purposes. Fr994, a cell line derived from ovaries of rainbow trout (Oncorhynchus mykiss), produced constantly high ISA virus (ISAV) titres and developed a pronounced CPE even at high cell passage numbers, while standard cell lines are reported to gradually loose these properties upon propagation. Another cell line isolated from the head kidney of common carp (Cyprinus carpio), KoK, showed a KHV induced CPE earlier than the standard cell line used for diagnostics. A third cell line, named Fin‐4, established from the fin epithelium of rainbow trout did not promote efficient replication of tested viruses, but showed antigen sampling properties and might be useful as an in vitro model for virus uptake or phagocytosis.

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“…Many viruses are highly dependent on host lipid metabolism for replication [27]. Lipid metabolism is therefore considered as a prime target for BSAA.…”

Section: Modulators Of Lipid Metabolismmentioning

confidence: 99%