THP-1 cell line: An in vitro cell model for immune modulation approach

Chanput, Wasaporn; Mes, Jurriaan J.; Wichers, Harry J.

doi:10.1016/j.intimp.2014.08.002

Cited by 879 publications

(691 citation statements)

References 108 publications

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“…It is a human leukemia monocytic cell line considered as a suitable, reliable model to study the functions and responses of monocytes and macrophages (Chanput et al, 2014). Herbal drugs have been used since traditional times in the treatment of various diseases.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Potential of Terminalia chebula by Hydrogen Peroxide Induced Oxidative Stress in THP-1 Cell Line

Minakshi¹,

Vasanth²,

Tanupriya³

et al. 2014

International J. of Botany

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A B S T R A C T Diseases caused due to 'oxidative stress' initiated by Reactive Oxygen Species (ROS) is growing at an alarming rate. Several medicinal herbs explored till date were reported to possess numerous pharmacological properties used for combating different diseases. The present study was undertaken to investigate the inhibitory potential of Terminalia chebula by H 2 O 2 induced oxidative stress in THP-1 cell line and also the presence of the marker compounds in T. chebula was confirmed by High Performance Liquid Chromatography (HPLC). Intracellular quantification of ROS was done using a flow cytometer. The percentage of gallic acid and ellagic acid in the extract was performed by HPLC were found to be 4.84% w/w and 2.56% w/w, respectively. Pretreatment of cells with T. chebula extract significantly reduced the intracellular ROS production in a dose-dependent manner. The present study illustrates the effective role of T. chebula in reducing the ROS production which is a causative factor for several disorders. The results describe the possibility and potentiality of natural sources to inhibit the pathogenesis of different diseases caused due to oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Potential of Terminalia chebula by Hydrogen Peroxide Induced Oxidative Stress in THP-1 Cell Line

Minakshi¹,

Vasanth²,

Tanupriya³

et al. 2014

International J. of Botany

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“…THP-1 cells are a monocytic cell line derived from the peripheral blood of a 1-year old male patient who had acute monocytic leukemia [216]. The difference between THP-1 cells and U-937 cells is the origin and maturation stage of both cell lines.…”

Section: U-937 and Thp-1mentioning

confidence: 99%

“…THP-1 cells are derived from a blood leukemia which represents a less mature stage. There is extensive literature describing the use of vitamin D3 or PMA to differentiate THP-1 cells into macrophages [216]. Recent literature has determined a protocol for PMA that seems to be the most effective to allow differentiation of THP-1 monocytes into macrophages [217].…”

Section: U-937 and Thp-1mentioning

confidence: 99%

Myelomonocytic Cell Lines in Modeling HIV-1 Infection of the Bone Marrow

Nonnemacher¹,

Quiterio²,

Allen³

et al. 2017

Biology of Myelomonocytic Cells

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Human immunodeficiency virus type 1 (HIV-1), the etiologic agent of acquired immunodeficiency syndrome (AIDS), primarily infects T cells and cells of the monocyte-macrophage lineage. This is due to the presence of the cell surface receptor CD4 and the coreceptors, CXCR4, and CCR5. While the T-cell has classically been the cell type associated with HIV-1 disease progression, cells of the monocyte-macrophage lineage have also been shown to play a major role in this viral pathologic process. Classically, this has involved monocytic cells in the peripheral blood and tissue macrophages, however, over the course of HIV disease, the promyelomonocytic cells of the bone marrow (BM) have also been shown to play a role in pathogenesis retroviral disease in that they play an integral role in the reseeding of the periphery and end-organ tissues. This has involved an initial infection of the bone marrow hematopoietic progenitor cells. Given this observation, over the years there have been a number of cell lines that have been developed and provided valuable insights into research questions surrounding HIV-1 infection of the monocyte-macrophage cell lineage. In this regard, we will examine the biological and immunological properties of these BM-derived cell lines with respect to their utility in exploring the pathogenesis of HIV-1 in humans.

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“…Louis, MO, USA), and 50 mg/ml Gentamycin (Daropakhsh, Tehran, IRAN), Penicillin 100 U/ml, Streptomycin 100 µ/ml (BIO IDEA, Tehran, IRAN), Amphotericin B 0.25 µg/ml (Cipla, Mumbai, INDIA) Cells were pelleted via centrifugation and assessed for viability using the Trypan-blue exclusion method. Viable cells were plated in six-well plates at a density of 5×105 cells/well and treated the same day [29] .…”

Section: Human Thp-1 Monocyte/macrophage Like Cells (Pasteur Institutmentioning

confidence: 99%

“…The synovial membrane is normally a thin membrane consisting of a lining and a sublining layer of cells. The lining consists of 1-3 layers of fibroblast-like synoviocytes (FLS) and macrophage-like synoviocytes (MLS) that overlay the sublining [32] . In the healthy synovium, the FLS express Cadherin-11 (Cad-11) as the only cell type, whereas MLS express CD14 and CD68 [33,34] .…”

Section: Characterization Of Bovine Fibroblast-like Synoviocytementioning

confidence: 99%

Down regulation of COX-2, IL-1β, TNF-α in cynoviocyte by essential oil of Fraxinus excelsior

Maghsoudi¹,

Haj-allahyari²

2018

Int J Cont Res Rev

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Abstract:Osteoarthritis (arthritis) is biomechanical, biochemical and cellular phenomenon, and is not known as a degenerative disease. Arthritis is one of the common chronic diseases and the most important reason of physical disability in the world. According to its side effect such as peptic ulcers, gastrointestinal bleeding, liver toxicity and renal complications dueofprescribing current treatment contain corticosteroid and nonsteroidal, we decided to evaluate possible effect of anti-inflammatory Esential oil of Fraxinus excelsior (EOFE) on biomarkers involved in disease. EOFE were prepared of genetic resources center. Bovine articular cartilage derived from the metacarpophalangeal joints of 14-18-month-old animals (without any sign of inflammation and bleeding) sent to laboratory in sterile bags at 4ºC. Cells were cultured in appropriate condition and counted by hemocytometer, viability assessed by trypan blue. After LPS treatment, cytokine levels were assayed. Cells cultures again and were kept in 37C, 90% humidity in CO 2 incubator and after RNA extraction, RT-PCR and PCR done. Also by Real-time PCR, gene expression was evaluated. E.E.F.E level cause down regulation of COX-2, IL-1β, TNF-α in LPS-stimulated cells.

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THP-1 cell line: An in vitro cell model for immune modulation approach

Cited by 879 publications

References 108 publications

Inhibitory Potential of Terminalia chebula by Hydrogen Peroxide Induced Oxidative Stress in THP-1 Cell Line

Inhibitory Potential of Terminalia chebula by Hydrogen Peroxide Induced Oxidative Stress in THP-1 Cell Line

Myelomonocytic Cell Lines in Modeling HIV-1 Infection of the Bone Marrow

Down regulation of COX-2, IL-1β, TNF-α in cynoviocyte by essential oil of Fraxinus excelsior

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