Natural populations of Spodoptera exigua are infected by multiple viruses that are transmitted to their offspring

Virto, Cristina; Navarro, David; Tellez, María del Mar; Herrero, Salvador; Williams, Trevor; Murillo, Rosa; Caballero, Primitivo

doi:10.1016/j.jip.2014.07.007

Cited by 53 publications

(52 citation statements)

References 33 publications

(11 reference statements)

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“…In the honey bee, several dicistrovirids and iflaviruses frequently infect the host simultaneously [13]. In the beet armyworm, iflaviruses can co-infect with baculovirids or other small RNA viruses [14,15]. In the fire ant, the aparavirus Solenopsis invicta virus-1 (SeIV1) can infect simultaneously with other picorna-like viruses [16]; and recently, 3 iflaviruses have been found in the brown planthopper [12 ,17].…”

Section: Introductionmentioning

confidence: 99%

Challenges associated with research on RNA viruses of insects

Carrillo-Tripp

Bonning

Miller

2015

Current Opinion in Insect Science

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Section: Introductionmentioning

confidence: 99%

Challenges associated with research on RNA viruses of insects

Carrillo-Tripp

Bonning

Miller

2015

Current Opinion in Insect Science

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“…The effectiveness of baculovirus-based insecticides relies on a diversity of virus-related and environmental factors [14], but also on the susceptibility of the pest population to baculovirus applications, which can be altered by cryptic infections involving other types of viruses [23, 41]. Here we demonstrated that the insecticidal properties of SeMNPV OBs were altered when the baculovirus replicated in hosts that were also infected by the iflaviruses SeIV-1 or SeIV-2.…”

Section: Discussionmentioning

confidence: 85%

“…Specific primers were used for the detection of SeMNPV DNA polymerase [23] and iflavirus RNA-dependent RNA polymerase (RdRp) [24] sequences (Table 1). Total DNA or cDNA was used as template for amplification in order to detect SeMNPV and iflaviruses, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Field-caught adults of S . exigua were found to be infected with both SeMNPV and two iflaviruses, and their laboratory-reared offspring also showed a high prevalence of both viruses [23, 24]. Recent studies on the association between SeMNPV and iflaviruses revealed that genomes of both types of viruses were present in the OBs of SeMNPV produced in insects that were infected by both viruses.…”

Section: Introductionmentioning

confidence: 99%

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Co-infection with iflaviruses influences the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus occlusion bodies: Implications for the production and biosecurity of baculovirus insecticides

et al. 2017

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Biological insecticides based on Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) can efficiently control S. exigua larvae on field and greenhouse crops in many parts of the world. Spanish wild populations and laboratory colonies of S. exigua are infected by two iflaviruses (SeIV-1 and SeIV-2). Here we evaluated the effect of iflavirus co-infection on the insecticidal characteristics of SeMNPV occlusion bodies (OBs). Overall, iflavirus co-inoculation consistently reduced median lethal concentrations (LC50) for SeMNPV OBs compared to larvae infected with SeMNPV alone. However, the speed of kill of SeMNPV was similar in the presence or absence of the iflaviruses. A reduction of the weight gain (27%) associated with iflavirus infection resulted in a 30% reduction in total OB production per larva. Adult survivors of SeMNPV OB inoculation were examined for covert infection. SeMNPV DNA was found to be present at a high prevalence in all SeIV-1 and SeIV-2 co-infection treatments. Interestingly, co-inoculation of SeMNPV with SeIV-2 alone or in mixtures with SeIV-1 resulted in a significant increase in the SeMNPV load of sublethally infected adults, suggesting a role for SeIV-2 in vertical transmission or reactivation of sublethal SeMNPV infections. In conclusion, iflaviruses are not desirable in insect colonies used for large scale baculovirus production, as they may result in diminished larval growth, reduced OB production and, depending on their host-range, potential risks to non-target Lepidoptera.

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“…This notwithstanding, deletions and/or insertions are known to result in several virus strains, e.g. for baculoviruses (Barrera et al, 2013;Ló pez-Ferber et al, 2003;Virto et al, 2014). Furthermore, such variation may play important roles in virus pathogenesis (Bernal et al, 2013;Clavijo et al, 2010;Simó n et al, 2004Simó n et al, , 2005.…”

Section: Genetic Heterogeneity Of the Gpsghv-eth Genomementioning

confidence: 99%

Comprehensive annotation of Glossina pallidipes salivary gland hypertrophy virus from Ethiopian tsetse flies: a proteogenomics approach

Abd-Alla

Kariithi

François

et al. 2016

Journal of General Virology

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Glossina pallidipes salivary gland hypertrophy virus (GpSGHV; family Hytrosaviridae) can establish asymptomatic and symptomatic infection in its tsetse fly host. Here, we present a comprehensive annotation of the genome of an Ethiopian GpSGHV isolate (GpSGHV-Eth) compared with the reference Ugandan GpSGHV isolate (GpSGHV-Uga; GenBank accession number EF568108). GpSGHV-Eth has higher salivary gland hypertrophy syndrome prevalence than GpSGHV-Uga. We show that the GpSGHV-Eth genome has 190 291 nt, a low G+C content (27.9 %) and encodes 174 putative ORFs. Using proteogenomic and transcriptome mapping, 141 and 86 ORFs were mapped by transcripts and peptides, respectively. Furthermore, of the 174 ORFs, 132 had putative transcriptional signals [TATA-like box and poly(A) signals]. Sixty ORFs had both TATA-like box promoter and poly(A) signals, and mapped by both transcripts and peptides, implying that these ORFs encode functional proteins. Of the 60 ORFs, 10 ORFs are homologues to baculovirus and nudivirus core genes, including three per os infectivity factors and four RNA polymerase subunits (LEF4, 5, 8 and 9). Whereas GpSGHV-Eth and GpSGHV-Uga are 98.1 % similar at the nucleotide level, 37 ORFs in the GpSGHV-Eth genome had nucleotide insertions (n517) and deletions (n520) compared with their homologues in GpSGHV-Uga. Furthermore, compared with the GpSGHV-Uga genome, 11 and 24 GpSGHV ORFs were deleted and novel, respectively. Further, 13 GpSGHV-Eth ORFs were non-canonical; they had either CTG or TTG start codons instead of ATG. Taken together, these data suggest that GpSGHV-Eth and GpSGHV-Uga represent two different lineages of the same virus. Genetic differences combined with host and environmental factors possibly explain the differential GpSGHV pathogenesis observed in different G. pallidipes colonies.

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Natural populations of Spodoptera exigua are infected by multiple viruses that are transmitted to their offspring

Cited by 53 publications

References 33 publications

Challenges associated with research on RNA viruses of insects

Challenges associated with research on RNA viruses of insects

Co-infection with iflaviruses influences the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus occlusion bodies: Implications for the production and biosecurity of baculovirus insecticides

Comprehensive annotation of Glossina pallidipes salivary gland hypertrophy virus from Ethiopian tsetse flies: a proteogenomics approach

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