Determination of the plasma global antioxidant capacity: a critical review

Pincemaïl, Joël; Cillard, Josiane; Nève, Jean; Defraigne, Jean-Olivier

doi:10.1684/abc.2014.0971

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several spectrophotometric methods have been developed to measure plasma or serum TAC, such as Oxygen Radical Absorbance Capacity (ORAC), Total Radical-trapping Antioxidant Parameter (TRAP), and 2,2 -Azinobis-(3-Ethylbenzothiazolin-6-Sulfonic Acid (ABTS) [27], which should be more precisely renamed plasma or serum non-enzymatic antioxidant capacity (NEAC) [28]. A major drawback of these assays is that uric acid, the major antioxidant in plasma, reacts strongly with the free radicals used, thus masking the effects of other antioxidants such as vitamins C and E [29,30]. Recently, Carrion-Garcia et al [28] also reported a strong and significant correlation (r = 0.67, p value 2.02 × 10 −19 ) between uric acid and TAC, as assessed by the reduction of ferric iron (Fe 3+ ) to ferrous iron (Fe 2+ ) by antioxidants present in plasma samples (Ferric Reducing Antioxidant Power (FRAP)).…”

Section: Introductionmentioning

confidence: 99%

A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT® Technology for Assessing Total Anti-Oxidative Capacity

Pincemaïl

Rousseau

Kaux³

et al. 2023

Biomedicines

Self Cite

View full text Add to dashboard Cite

Background: Oxidative stress (OS) could cause various COVID-19 complications. Recently, we have developed the Pouvoir AntiOxydant Total (PAOT®) technology for reflecting the total antioxidant capacity (TAC) of biological samples. We aimed to investigate systemic oxidative stress status (OSS) and to evaluate the utility of PAOT® for assessing TAC during the recovery phase in critical COVID-19 patients in a rehabilitation facility. Materials and Methods: In a total of 12 critical COVID-19 patients in rehabilitation, 19 plasma OSS biomarkers were measured: antioxidants, TAC, trace elements, oxidative damage to lipids, and inflammatory biomarkers. TAC level was measured in plasma, saliva, skin, and urine, using PAOT and expressed as PAOT-Plasma, -Saliva, -Skin, and -Urine scores, respectively. Plasma OSS biomarker levels were compared with levels from previous studies on hospitalized COVID-19 patients and with the reference population. Correlations between four PAOT scores and plasma OSS biomarker levels were analyzed. Results: During the recovery phase, plasma levels in antioxidants (γ-tocopherol, β-carotene, total glutathione, vitamin C and thiol proteins) were significantly lower than reference intervals, whereas total hydroperoxides and myeloperoxidase (a marker of inflammation) were significantly higher. Copper negatively correlated with total hydroperoxides (r = 0.95, p = 0.001). A similar, deeply modified OSS was already observed in COVID-19 patients hospitalized in an intensive care unit. TAC evaluated in saliva, urine, and skin correlated negatively with copper and with plasma total hydroperoxides. To conclude, the systemic OSS, determined using a large number of biomarkers, was always significantly increased in cured COVID-19 patients during their recovery phase. The less costly evaluation of TAC using an electrochemical method could potentially represent a good alternative to the individual analysis of biomarkers linked to pro-oxidants.

show abstract

Section: Introductionmentioning

confidence: 99%

A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT® Technology for Assessing Total Anti-Oxidative Capacity

Pincemaïl

Rousseau

Kaux³

et al. 2023

Biomedicines

Self Cite

View full text Add to dashboard Cite

show abstract

“…Using a rat model, Gerardi et al [ 22 ] evidenced that the antioxidant capacity of plasma as measured by DPPH and FRAP assays was increased after intake of pomace products in a dose-response effect. Nevertheless, the use of both assays to evidence in vivo antioxidant activity largely remains a matter of debate [ 23 ]. In a more accurate way, Curti et al [ 24 ] showed in a mouse model that oral administration of whole brown propolis extract containing galangin is followed by rapid absorption and metabolization of the latter, resulting in adaptations of the antioxidant enzymatic defense system.…”

Section: Introductionmentioning

confidence: 99%

Ex Vivo Antioxidant Capacities of Fruit and Vegetable Juices. Potential In Vivo Extrapolation

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background: In support of claims that their products have antioxidant properties, the food industry and dietary supplement manufacturers rely solely on the in vitro determination of the ORAC (oxygen radical antioxidant capacity) value, despite its acknowledged lack of any in vivo relevance. It thus appears necessary to use tests exploiting biological materials (blood, white blood cells) capable of producing physiological free radicals, in order to evaluate more adequately the antioxidant capacities of foods such as fruit and vegetable juices. Materials: Two approaches to assessing the antioxidant capacities of 21 commercial fruit and vegetable juices were compared: the ORAC assay and the “PMA–whole blood assay,” which uses whole blood stimulated by phorbol myristate acetate to produce the superoxide anion. We described in another paper the total polyphenol contents (TPCs) and individual phenolic compound contents of all the juices investigated here (Matute et al. Antioxidants 2020, 9, 1–18). Results: Ranking of the juices from highest to lowest antioxidant capacity differed considerably according to the test used, so there was no correlation (r = 0.33, p = 0.13) between the two assays when considering all juices. Although the results of the ORAC assay correlated positively with TPC (r = 0.50, p = 0.02), a much stronger correlation (r = 0.70, p = 0.004) emerged between TPC and % superoxide anion inhibition. In the PMA–whole blood assay, peonidin-3-O-glucoside, epigallocatechin gallate, catechin, and quercetin present in juices were found to inhibit superoxide anion production at concentrations below 1 µM, with a strong positive correlation. Conclusions: Associated with the determination of total and individual phenolic compounds contained in fruit and vegetable juices, the PMA–whole blood assay appears better than the ORAC assay for evaluating juice antioxidant capacity.

show abstract

“…The most popular test is the ORAC (oxygen radical antioxidant capacity) assay which evaluates how antioxidants present in biological samples can inhibit, or not, physiological free radical species produced in a test tube. A very strong limitation of such an approach is that the plasma antioxidant capacity is dependent on uric acid and total protein concentrations of more than 70% [8,9].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Methodology for Evaluating Skin Oxidative Stress Status (SOSS)

Pincemaïl

Kaci²,

Cheramy-Bien

et al. 2019

Diseases

View full text Add to dashboard Cite

For the purpose of human disease prevention, several methods have been developed, and are still developing, to assess the oxidative stress status (OSS) of individuals. In the present paper, we describe an approach based on electrochemical detection able to evaluate skin oxidative stress status (SOSS) as a PAOT (Pouvoir AntiOxydant Total)-Skin Score®. Normal reference values for the PAOT-Skin Score® were: 0–62.94 (n = 263). Intra- and inter-assay coefficients of variation were, respectively, 12.47 ± 4.29% and 7.0 ± 2.5%. Our technology showed increased skin antioxidant activity following topical application of reduced coeznyme Q10 cream or vitamin C intake as orange juice or supplements. Moreover, we found significant correlations between some blood oxidative stress biomarkers and the PAOT-Skin Score® (γ-tocopherol/α-tocopherol ratio (r = 0.43, p = 0.020); copper (r = −0.42, p = 0.022); copper/zinc ratio (r = −0.49, p = 0.006), and lipid peroxides (r = −0.43, p = 0.002)). In addition to being non–invasive, the present electrochemical methodology is also not expensive, fast, and easy to use.

show abstract

Determination of the plasma global antioxidant capacity: a critical review

Cited by 3 publications

References 0 publications

A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT® Technology for Assessing Total Anti-Oxidative Capacity

A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT® Technology for Assessing Total Anti-Oxidative Capacity

Ex Vivo Antioxidant Capacities of Fruit and Vegetable Juices. Potential In Vivo Extrapolation

Electrochemical Methodology for Evaluating Skin Oxidative Stress Status (SOSS)

Contact Info

Product

Resources

About