High frequency of bovine viral diarrhea virus type 2 in Southern Brazil

Weber, Matheus Nunes; Silveira, Simone; Machado, Gustavo; Groff, F.H.S.; Mósena, Ana Cristina Sbaraini; Budaszewski, R.F.; Dupont, Priscilla Medeiros; Corbellini, Luís Gustavo; Canal, Cláudio Wageck

doi:10.1016/j.virusres.2014.07.035

Cited by 43 publications

(67 citation statements)

References 29 publications

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“…An investigation on a Brazilian dairy herd consisting of 692 animals identified 4 PI animals which were subsequently typed as BVDV-1a (n=1), 1b (n=2) and 1d (n=1) (Otonel et al 2014). The circulation of these three BVDV-1 subgenotypes was consistent with the findings of Weber et al (2014). The screening of a Brazilian herd experiencing reproductive problems identified four PI animals infected with BVDV-1b (n=2), 1d (n=1) and 1a (n=1).…”

Section: Prevalence Of Bvdv Genotypes and Sub-genotypesmentioning

confidence: 66%

“…BVDV-1 (n=19) was marginally dominant over BVDV-2 (14) with BVDV-1a the most frequently observed sub-genotype. BVDV-1b and 1d were also documented (Weber et al 2014). An investigation on a Brazilian dairy herd consisting of 692 animals identified 4 PI animals which were subsequently typed as BVDV-1a (n=1), 1b (n=2) and 1d (n=1) (Otonel et al 2014).…”

Section: Prevalence Of Bvdv Genotypes and Sub-genotypesmentioning

confidence: 98%

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Genetic typing of bovine viral diarrhoea virus in cattle on Irish farms

O’Brien

Garvey

Walsh

et al. 2017

Research in Veterinary Science

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The genotype and subgenotype was established for 325 field viruses and one commercial vaccine viral strain based on the analysis of at least one genomic region. BVDV-1a was the prominent subgenotype (n=317) with BVDV-1b (n=6), BVDV-1d (n=1) and BVDV-1e (n=1) also observed. A number of nucleotide sequence alignments and phylogenetic trees were constructed to represent the relationship between Irish field viruses and reference strains representative of all known BVDV genotypes and subgenotypes. Evidence of both herd specific clustering and circulation of multiple strains within herds was observed; however no evidence of county specific clustering was observed. A number of completely conserved nucleotide regions were observed and the 5'UTR was found to be more conserved than the N pro fragment analysed. The results of this study provide a detailed and comprehensive insight into the genetic diversity of BVDV in circulation within Irish cattle herds and will act as a baseline reference for future BVDV genotyping studies.

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Section: Prevalence Of Bvdv Genotypes and Sub-genotypesmentioning

confidence: 66%

Section: Prevalence Of Bvdv Genotypes and Sub-genotypesmentioning

confidence: 98%

Genetic typing of bovine viral diarrhoea virus in cattle on Irish farms

O’Brien

Garvey

Walsh

et al. 2017

Research in Veterinary Science

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“…The virus used for experimentally infecting the animals was originally isolated from the blood of an animal belonging to a herd with reproductive problems (BIANCHI et al, 2011). After isolation in cell culture, the 5′ UTR region was sequenced according to WEBER et al (2014) and the viral isolate was classified as BVDV-1a.…”

Section: Cell Culture and Virusmentioning

confidence: 99%

“…Genetic and antigenic characterization of virus samples from these animals indicates a wide variability and a predominant presence of BVDV-1 (54%), apart from BVDV-2 (34%) and the Hobi-like virus (12%) (BOTTON et al, 1998;CORTEZ et al, 2006;BIANCHI et al, 2011;WEBER et al, 2014). The most prevalent subgenotypes in circulation are BVDV-1a (36%), BVDV-2b (31%), and BVDV-1d (10%) (WEBER et al, 2014;SILVEIRA et al, 2015). The BVDV-2 strain is more prevalent among Brazilian cattle compared with cattle from other regions of the world (WEBER et al, 2014;SILVEIRA et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…The most prevalent subgenotypes in circulation are BVDV-1a (36%), BVDV-2b (31%), and BVDV-1d (10%) (WEBER et al, 2014;SILVEIRA et al, 2015). The BVDV-2 strain is more prevalent among Brazilian cattle compared with cattle from other regions of the world (WEBER et al, 2014;SILVEIRA et al, 2015). In the early 2000s, the presence of the Hobilike virus was detected in Brazilian herds; however, its prevalence was much less compared with that of BVDV genotypes 1 and 2 (CORTEZ et al, 2006;BIANCHI et al, 2011;WEBER et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Clinical and virological characteristics of calves experimentally infected with a Brazilian isolate of bovine viral diarrhea virus type 1a

et al. 2016

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To study the pathogenicity of the Brazilian bovine viral diarrhea virus (BVDV) type 1a 241.10 isolate, four calves were intranasally inoculated with a viral suspension containing 107.2 TCID50 mL-1. One calf was left uninoculated and kept in contact with the other calves to investigate viral transmissibility. After inoculation, the animals were monitored daily for clinical signs of infection. The presence of the virus in the blood and nasal secretions was confirmed by virus isolation in cell culture. White blood cells were quantified prior to and every 3 days after infection, and the presence of antibodies was checked every 7 days, starting at day 0 until day 42 post-inoculation (pi). After infection, nasal and ocular serous secretions were observed between days 1 and 5 pi, along with a mild cough from days 2 to 4 pi; however, no severe clinical signs were present. Body temperature was slightly elevated between days 4 and 6 pi. The control calf did not develop any of the signs observed in the infected animals. Cell culture-mediated virus isolation confirmed viremia between days 4 and 8 pi and the presence of the virus in the nasal secretions between days 1 and 10 pi. All infected animals showed a decrease in white blood cell count. Antibodies could be detected from day 14 pi, and these levels remained high until day 35 pi. The control calf had no viremia, viral presence in nasal secretions, or positive serology, indicating the absence of viral transmission. Thus, isolate BVDV 1a 241.10 has low pathogenicity and transmissibility but retains immunosuppressive capacity.

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Molecular diversity of bovine viral diarrhea virus in uruguay

Maya

Puentes

Reolón³

et al. 2015

Arch Virol

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Bovine viral diarrhea (BVD) affects bovine production and reproduction causing significant economic losses all over the world. Two viral species has been recognized: BVDV-1 and BVDV-2, both distributed worldwide. Recently, novel specie of BVDV named HoBi-like pestivirus was discovered. The presence of BVDV was confirmed in 1996 in Uruguay, however, does not exist until today a schedule of compulsory vaccination along the country. Serological studies with samples from all Uruguayan herds were performed during 2000 and 2001 demonstrating that all of them were seropositive to BVDV with a mean prevalence of 69%. In addition, there have been no new studies done since those previously described and it is important to mention that the genetic diversity of BVD has never been described in Uruguay. Nowadays, there is strongly suspect that BVDV is one of the most important causes of reproductive failures in our herds. The aim of this study was to describe for the first time in Uruguay the genetic diversity of BVDV with samples collected from different regions along the country. Serological status of 390 non-vaccinated animals against BVDV with reproductive problems from farms of Rivera, Tacuarembó and Florida departments of Uruguay were studied. All herds were seropositive to BVDV and high proportion of animals were positive (298/390), while 4.1% (16/390) of the animals were positive to Antigen Capture ELISA test and Real Time PCR. Phylogenetic analysis performed with concatenated sequences from the 5'UTR and Npro genomic regions revealed that BVDV-1 and BVDV-2 are infecting our herds, being BVDV-1 the most frequently found. The major subtype was BVDV-1a, followed by BVDV-1i and BVDV-2b. This is the first study that describes the genetic diversity of BVDV in Uruguay and it will contribute to the elaboration of sanitization programs.

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High frequency of bovine viral diarrhea virus type 2 in Southern Brazil

Cited by 43 publications

References 29 publications

Genetic typing of bovine viral diarrhoea virus in cattle on Irish farms

Genetic typing of bovine viral diarrhoea virus in cattle on Irish farms

Clinical and virological characteristics of calves experimentally infected with a Brazilian isolate of bovine viral diarrhea virus type 1a

Molecular diversity of bovine viral diarrhea virus in uruguay

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