251: Clinical relevance of crossmatch results, HLA and non-HLA Ab antibodies for cardiac allograft recipients

Immunosuppression in the sensitized heart transplant recipient

“…An antibody testing exercise was recently reported in which eight laboratories all determined the same specificity, but the distribution of MFI varied over four-fold [15 ], highlighting the challenge. An MFI of more than 10 000 has been found by one group to highly correlate with cytoxicity [16]; good outcomes have also been reported despite low-titer DSA [17]. Unfortunately, even with SPAs, antigen density of single-antigen beads is not sufficiently consistent to permit reproducible quantification, thereby precluding a standard threshold.…”