Short communication: Antimicrobial susceptibility profiling and genotyping of Staphylococcus aureus isolates from bovine mastitis in Poland

Jagielski, Tomasz; Puacz, Elżbieta; Lisowski, A.; Siedlecki, Paweł; Dudziak, W.; Międzobrodzki, Jacek; Krukowski, Henryk

doi:10.3168/jds.2014-8321

Cited by 27 publications

(23 citation statements)

References 28 publications

(53 reference statements)

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“…S. aureus isolated from animals with clinical and subclinical mastitis exhibit high rates of resistance to beta-lactam antibiotics (Kalmus et al, 2011;El Behiry et al, 2012;Silva et al, 2012;Jagielski et al, 2014;Schmidt et al, 2015). A considerable rate of simultaneous resistance to beta-lactamases amoxicillin, ampicillin and penicillin was found in 69.03% (18/26) of S. aureus isolates in this study (Table 1).…”

Section: Resultsmentioning

confidence: 56%

Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis

Xavier¹,

Almeida²,

Souza³

et al. 2017

Genet. Mol. Res.

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The Staphylococcus aureus is the most common isolated microorganism in ruminant animal species diagnostic with clinical or subclinical mastitis. Dairy herds with these diseases can transfer S. aureus into the milk supply, which can lead to food poisoning in humans. The objective of this study was to evaluate the profile of antimicrobial susceptibility, the presence of femA gene, the genetic relationships among isolates of S. aureus obtained from milk originating from flocks diagnosed with subclinical mastitis in nine rural properties in the northern of Minas Gerais State. To this end, 498 samples of bovine milk tested positive for the California mastitis test (CMT) were subjected to morphological methods and biochemical patterns for microbiological presumptive identification of S. aureus. The PCR test with the genetic marker femA was used to confirm the species S. aureus. All the 26 isolates presumptively identified as S. aureus amplified a fragment of 132 bp corresponding to the femA gene. The profile of antimicrobial susceptibility was performed according to the disk-diffusion methodology and two isolates were susceptible to all the antibiotics tested. The drug multiresistence was found in 80.76% of the isolates. The determination of the genetic profile and the clonal relationship among the isolates was performed by the method of DNA RAPD-PCR polymorphism. The S. aureus isolates were divided into two groups with 26 distinct subgroups. The analysis of RAPD-PCR showed no genetic diversity among them, heterogeneous profile and absence of clonality.

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Section: Resultsmentioning

confidence: 56%

Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis

Xavier¹,

Almeida²,

Souza³

et al. 2017

Genet. Mol. Res.

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“…To date, the issue of resistant or multiresistant MCP such as methicillin‐resistant S. aureus (MRSA) seems to play a subordinate role in dairy industry (Erskine et al., ; Kreausukon et al., ; Oliver, Murinda, & Jayarao, ; Pol & Ruegg, ; Schröder, Hoedemaker, & Klein, ; Tenhagen et al., ). However, the number of studies reporting of enhanced emergence of AMR increases (da Costa Krewer et al., ; Ding et al., ; Fairbrother et al., ; Jagielski et al., ; Petrovski et al., ; Ruegg, Oliveira, Jin, & Okwumabua, ; Saini, McClure, Scholl, DeVries, & Barkema, ; Spohr et al., ).…”

Section: Mastitis Management—therapeutic Measuresmentioning

confidence: 99%

Mastitis treatment—Reduction in antibiotic usage in dairy cows

Krömker¹,

Leimbach²

2017

Reprod Domestic Animals

200

161

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Contents Animal‐friendly, economical, resource‐saving milk production provides the basis for sustained consumer acceptance. Bovine mastitis plays a decisive role in the dairy industry—disturbing animal health and welfare and causing considerable economic losses on the other hand. Currently, antimicrobial treatment is indispensable to keep bovine udder health, animal welfare and economic aspects in balance. On the contrary, emergence and spread of antimicrobial resistance (AMR) is an urgent matter of particular public interest, and as a consequence, antimicrobial usage (AMU) in production livestock is a critically discussed subject. In urgent need of future reduction in AMU in the dairy industry, this review article describes and discusses possible approaches promising prompt implementation, including therapeutical alternatives as well as pro‐ and metaphylactic concepts such as the implementation of evidence‐based mastitis therapy concepts and selective dry cow treatment (sDCT), in search of the most effective and contemporary methods for decreasing AMU and AMR in dairy production.

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“…Resistance to β-lactam antibiotics has been also reported as one of the main problems related to the persistence of mastitis [6,7]. Multidrug resistance is also reported to be increasing.…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of methicillin-susceptible Staphylococcus aureus (MSSA) isolated from milk of cows with subclinical mastitis

Bonsaglia

Silva

Rossi

et al. 2018

Microbial Pathogenesis

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Bovine mastitis has been a concern for dairy herd for decades. The adaptation capacity of one of the main species responsible for this disease, Staphylococcus aureus (S. aureus), plays a pivotal role in this issue. The aim of this study was to establish a molecular and phenotypic profile of 285 S. aureus strains isolated from milk of subclinical mastitis cows from 18 different farms in São Paulo State using spa typing, multilocus sequence typing (MLST), pulsed field gel electrophoresis (PFGE), agr cluster (I, II, III and IV) typing, PCR for genes including enterotoxins (sea, seb, sec, sed, see, seg, seh, sei), toxic shock syndrome toxin (tsst-1), and Panton-Valentine leucocidin (pvl), as well as in vitro resistance assays for 12 antibiotics. The results showed a wide variety of strains with a high toxigenic potential; concomitantly, sec, seg and seh were prevalent. In addition, we observed a predominance of the spa types t605 (ST 126, CC126) and t127 (ST1, CC1) and the unusual presence of t321 causing bovine mastitis, which has been previously reported only in swine. The most frequent ST were ST126 (70.5%) and ST1 (10.5%). Regarding PFGE, we observed four major groups and six profile patterns. The highest resistance was observed for streptomycin (9.5%), followed by tetracycline (3.5%), clindamycin (9.3%), and erythromycin (2.8%). The tsst-1 gene was detected in 36.8% of isolates and pvl was not observed. One hundred and thirty-six (47.7%) isolates possessed agr type II, followed by types III (20%) and I (8.1%), with type IV not being detected. We observed that the same spa type could result in different PFGE profiles, so the exclusive use of spa type sequences can lead to incorrect interpretations regarding the spread of clones in an epidemiological context.

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Short communication: Antimicrobial susceptibility profiling and genotyping of Staphylococcus aureus isolates from bovine mastitis in Poland

Cited by 27 publications

References 28 publications

Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis

Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis

Mastitis treatment—Reduction in antibiotic usage in dairy cows

Molecular epidemiology of methicillin-susceptible Staphylococcus aureus (MSSA) isolated from milk of cows with subclinical mastitis

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